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CAG::PSAML141F,Y115F:5HT3HC-IRES-GFP

Plasmid

#32476

Purpose

Expresses PSAM-5HT3 HC (L141F,Y115F) neuronal activator, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAM Y115F,L141F-5HT3 HC

Use

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-EGFP

Plasmid

#122954

Purpose

Lentiviral expression of NOTCH2NLB full length under CAG promoter with EGFP expression

Depositor

Pierre Vanderhaeghen

Article

Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

Insert

NOTCH2NLB (NOTCH2NLB Human)

Use

Lentiviral

Tags

6xhis tag

Expression

Mutation

Promoter

CAG promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLX304 3xFlag-eRF1 G183/184A_IRES-GFP

Plasmid

#198394

Purpose

eRF1 (AAQ mutant) expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

eRF1 (ETF1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

mutated GGQ motif (G183/184A)

Promoter

CMV

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse Ezh2 Y637S-IRES-GFP

Plasmid

#91887

Purpose

Retroviral expression of mouse Ezh2 with Y637S

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Ezh2 (Ezh2 Mouse)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Y637S

Promoter

MMLV gag

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FKBP-C-mSOG-J-alpha-IRES-mCherry

Plasmid

#136603

Purpose

Encodes FKBP tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

Depositor

John Ngo

Article

Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

Insert

FKBP-C-mSOG-J-alpha

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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CaM1234(D20A/D56AD93A/D129A)/pIRES2-eGFP

Plasmid

#111518

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

calmodulin [Xenopus laevis] (calm2 Frog)

Use

Tags

Expression

Mammalian

Mutation

D20A/D56AD93A/D129A

Promoter

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-human CSFI-R-L301S-IRES-GFP

Plasmid

#133744

Purpose

retroviral expression of CSF1-R mutant

Depositor

Martine Roussel

Article

Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

Insert

CSF1-R (CSF1R Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

L301S

Promoter

MSCV

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6_IRES-mTurquoise2

Plasmid

#113890

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mTurquoise2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-BRD9_dBD-PGK-Puro-IRES-GFP

Plasmid

#75115

Purpose

Retroviral expression plasmid encoding a human BRD9 mutant lacking the bromodomain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Tags

Expression

Mutation

deleted bromodomain

Promoter

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pLenti NLuc398-hLGALS8 IRES hCALCOCO2-CLuc394

Plasmid

#128387

Purpose

Expresses a split firefly luciferase reporter based on the full length, human Gal8 and CALCOCO2, which interact following endosomal disruption

Depositor

Craig Duvall

Article

Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss

Inserts

NLuc398-3xGGGGS-LGALS8 (LGALS8 Human, Synthetic)
CALCOCO2-3xGGGGS-CLuc394 (CALCOCO2 Human)

Use

Lentiviral, Luciferase, and Synthetic Biology

Tags

CLuc394 and NLuc398

Expression

Mammalian

Mutation

Promoter

EFS and IRES

Available since

Jan. 17, 2020

Availability

Academic Institutions and Nonprofits only

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