We narrowed to 17,089 results for: EMB

1. pcDNA3.1/CIP2A(1-905) L533E V5 His

   Plasmid

   #119288

   Purpose

   CIP2A L533E V5 overexpression, CIP2A dimer defective mutant

   Depositor

   Jukka Westermarck

   Article

   Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

   Insert

   KIAA1524 (CIP2A Human)
   

   Tags

   V5 His

   Expression

   Mammalian

   Mutation

   L533E

   Promoter

   T7

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKK-TEV-mVenus

   Plasmid

   #105791

   Purpose

   Expression of your protein of interest in fusion with yellow fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-mVenus

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. MBP-FUS_LC_12E

   Plasmid

   #98654

   Purpose

   Expresses MBP-tagged phosphomimetic FUS AA 1-163

   Depositor

   Nicolas Fawzi

   Article

   Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

   Insert

   FUS (FUS Human)
   

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   12 S/T to E

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-myc-Miro1 Δ593-618

   Plasmid

   #47895

   Purpose

   Expresses myc tagged Miro1 lacking transmembrane domain

   Depositor

   Pontus Aspenström

   Article

   Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

   Insert

   Miro1 Δ593-618 (RHOT1 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Δ593-618, lacks transmembrane domain

   Promoter

   CMV

   Available Since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pTXB1-EXO1b D173A

   Plasmid

   #68269

   Purpose

   Bacterial expression of human EXO1 D173A (catalytically-dead) mutant

   Depositor

   Stefano Ferrari

   Article

   Eid et al EMBO Rep. 2010 Dec;11(12):962-8. doi: 10

   Insert

   EXO1 (EXO1 Human)
   

   Tags

   Mxe intein/chitin binding domain

   Expression

   Bacterial

   Mutation

   catalytically-dead D173A. Please see depositor co…

   Promoter

   T7

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

6. mCherry-CRY2-P14P5K

   Plasmid

   #79570

   Purpose

   expression of mCherry-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1c

   Depositor

   Olof Idevall-Hagren

   Article

   Xie et al Cell Chem Biol. 2016 Jul 21;23(7):816-26

   Insert

   Pip5k1c (Pip5k1c Mouse)
   

   Tags

   CRY2 (photolyase homology region domain of Arabid…

   Expression

   Mammalian

   Mutation

   R445E and R446E; truncation of amino acids 636-66…

   Promoter

   CMV

   Available Since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pHub10-His-YFP-PIP2;1

   Plasmid

   #99386

   Purpose

   overexpression and isolation of recombinant At-PIP2;1 protein

   Depositor

   Ning Li

   Article

   Qing et al Mol Plant. 2016 Jan 4;9(1):158-74. doi:

   Insert

   PIP2;1 (PIP2A Mustard Weed)
   

   Tags

   His and YFP

   Expression

   Plant

   Promoter

   2xCaMV35S

   Available Since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKK-mEGFP-TEV

   Plasmid

   #105777

   Purpose

   Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   mEGFP-TEV

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCVpuro-Gcn5(D608A)

   Plasmid

   #63707

   Purpose

   retroviral expression of enzyme-dead mouse Gcn5 in mammalian cells

   Depositor

   Kai Ge

   Article

   Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

   Insert

   Gcn5 (Kat2a Mouse)
   

   Use

   Retroviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   D608A mutation

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pRS423-mitoT

    Plasmid

    #174525

    Purpose

    Expresses yeast variant of mitoT synthetic intermembrane tether bridging the inner and outer mitochondrial membranes

    Depositor

    Oleh Khalimonchuk

    Article

    Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

    Insert

    Yeast mitochondrial intermembrane tether
    

    Use

    Synthetic Biology

    Tags

    eGFP

    Expression

    Yeast

    Promoter

    MET25

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTH728-CEN-RLuc/maxCFLuc

    Plasmid

    #38212

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-USPL1-C236A-pcDNA3.1

    Plasmid

    #85764

    Purpose

    mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236A

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, full length, catalytic inactive variant variant C236A (USPL1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    catalytic inactive mutant C236A

    Promoter

    CMV

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-USPL1-C236S-pcDNA3.1

    Plasmid

    #85763

    Purpose

    mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236S

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    catalytic inactive mutant C236S

    Promoter

    CMV

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1/CIP2A(1-905) R522D V5 His

    Plasmid

    #119289

    Purpose

    CIP2A R522D V5 overexpression, CIP2A dimer defective mutant

    Depositor

    Jukka Westermarck

    Article

    Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

    Insert

    KIAA1524 (CIP2A Human)
    

    Tags

    V5 His

    Expression

    Mammalian

    Mutation

    R522D

    Promoter

    T7

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKK-NoTag

    Plasmid

    #105767

    Purpose

    Control vector for pKK series (encodes only SLIC arms (TEV-L and TEV-R)); expression without tag. Useful to design other vectors; any tag can be added.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Expression

    Mammalian

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTH726-CEN-RLuc/minCFLuc

    Plasmid

    #38210

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-nAChr-Alpha6

    Plasmid

    #48812

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. FB009

    Plasmid

    #119707

    Purpose

    Transcriptional Unit (TU) for geneticin (G418) resistance in fungi obtained by combining FB001+FB005+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    PtrpC::nptII::Ttub
    

    Use

    Synthetic Biology

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKK-FLAG-TEV

    Plasmid

    #105768

    Purpose

    Expression of your protein of interest in fusion with FLAG at the N-terminus. The tag is cleavable by TEV protease or enterokinase.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-hPMCA2xb

    Plasmid

    #47751

    Purpose

    mammalian expression of mCherry tagged hPMCA2, xb splice variant

    Depositor

    Emanuel Strehler

    Article

    Paszty et al Sci Signal. 2015 Feb 17;8(364):ra19. doi

    Insert

    PMCA2x/b (ATP2B2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    xb splice variant

    Promoter

    CMV

    Available Since

    Nov. 23, 2018

    Availability

    Academic Institutions and Nonprofits only