We narrowed to 88,166 results for: Mal

1. SpCas9-CBE6c-V106W

   Plasmid

   #215826

   Purpose

   Express CBE6c V106W (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6c V106W-SpCas9-2xUGI (cas9 )
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. peGFP C3-SPG20

   Plasmid

   #218578

   Purpose

   Expresses GFP-tagged human SPG20 in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

   Insert

   SPG20 (SPART Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5-HA-Ubiquitin-WT

   Plasmid

   #17608

   Purpose

   Mammalian expression of HA tagged ubiquitin

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRX-IZ-ANXA11-mRuby3

   Plasmid

   #184894

   Purpose

   Stable expression of ANXA11-mRuby3 (Annexin A11-mRuby3) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

   Insert

   ANXA11 (ANXA11 Human)
   

   Use

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   R230C relative to NP_001148.1

   Promoter

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. RAMA-bio-His

   Plasmid

   #50737

   Purpose

   Expresses enzymatically monobiotinylated full length RAMA ectodomain

   Depositor

   Gavin Wright

   Article

   Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

   Insert

   RAMA
   

   Use

   Tags

   ratCD4d3+4,biotinylation sequence, 6xHis

   Expression

   Mammalian

   Mutation

   all potential N-linked glycosylation sites (N-X-S…

   Promoter

   CMV

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. LV_EF1a_SV40_Large_T_Antigen-P2A-Hygro_Barcode

   Plasmid

   #170255

   Purpose

   Barcoded lentiviral vector to express SV40 Large T Antigen in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   SV40 Large T Antigen
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS

   Plasmid

   #199349

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP-SUMOEu

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6

   Plasmid

   #190162

   Purpose

   Expresses 24 Broccoli-tagged beta-actin mRNA in mammalian cells

   Depositor

   Samie Jaffrey

   Article

   Li et al Angew Chem Int Ed Engl. 2020 Mar 9;59(11

   Insert

   mcherry_(3'UTR, No Actin)_24Broccoli
   

   Use

   Tags

   24×Broccoli

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB_tre_Cas9

   Plasmid

   #126029

   Purpose

   PiggyBac cargo vector expressing doxycycline inducible Cas9

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Inserts

   humanized S. pyrogenes Cas9
   Hygromycin resistance
   

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1 alpha and TRE

   Available Since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EFS-SpCas9

    Plasmid

    #104588

    Purpose

    AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

    Depositor

    Ryohei Yasuda

    Article

    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

    Insert

    Streptococcus pyogenes Cas9
    

    Use

    AAV and CRISPR

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWN_U6-B2M-miniGag-Cas9

    Plasmid

    #228958

    Purpose

    miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-SpdCas9-VP64

    Plasmid

    #115794

    Purpose

    Expresses SpdCas9-Vp64 fusion protein when packaged into AAV

    Depositor

    Nadav Ahituv

    Article

    Matharu et al Science. 2018 Dec 13. pii: science.aau06

    Insert

    VP64
    

    Use

    AAV

    Tags

    3XFLAG and dCas9-VP64

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-FlipCherry-T2A-GFP

    Plasmid

    #124436

    Purpose

    Expresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipCherry-T2A-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-SARS-CoV-2-S (Wuhan)

    Plasmid

    #215812

    Purpose

    Expresses SARS-CoV-2 Spike protein in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    SARS-CoV-2 Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-EF1A-Puro-hTBK1-EGFP

    Plasmid

    #221553

    Purpose

    Expresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Piggybac

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AMPK(T>A)-SPARK

    Plasmid

    #226256

    Purpose

    phospho-null mutant of AMPK-SPARK

    Depositor

    Roland Malli

    Article

    Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

    Insert

    AMPK(T>A)-SPARK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    substituted Threonine 20 with Alanine

    Promoter

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA 3.1(Hygro) myc-hPolQ-Flag

    Plasmid

    #73132

    Purpose

    To express in mammalian cells human PolQ

    Depositor

    Agnel Sfeir

    Article

    Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

    Insert

    Polymerase theta (POLQ Human)
    

    Use

    Tags

    Flag and myc

    Expression

    Mammalian

    Mutation

    Promoter

    cmv promoter

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-P4:gs:nls:CIBN

    Plasmid

    #135986

    Purpose

    Expresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cells

    Depositor

    Roman Jerala

    Article

    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

    Insert

    P4 coiled-coil fused to the CIBN domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT3-EF1aH NICD1

    Plasmid

    #86500

    Purpose

    Mammalian expression of Myc tagged NICD1

    Depositor

    Xin Chen

    Article

    Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

    Insert

    Notch1 (C-term) (Notch1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Cytoplasmic domain only (AA 1753-2531)

    Promoter

    EF1a

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV_NFL(WT)-FLAG

    Plasmid

    #182654

    Purpose

    Expresses wild-type mouse neurofilament light chain with a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    wild type mouse neurofilament light chain (Nefl Mouse)
    

    Use

    Tags

    FLAG tag (DYKDDDDK)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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