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pCFJ2441 - [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159870

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2443 - [1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159867

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2453 - [1-2] ENTR - Fluor - ce-mCardinal(900bp PATCs, NLS, no_atg, no_stop)

Plasmid

#159863

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mCardinal(900bp PATCs, NLS, no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AMPKsub-YPet-Kras

Plasmid

#84638

Purpose

Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

AMPKsub-YPet-Kras

Tags

6xHis, C-terminal targeting sequence from Kras, T…

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-MBP-GGSG-DmBam_13-36_AG

Plasmid

#148841

Purpose

Mammalian Expression of DmBam_13-36

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_13-36 (bam Fly)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-HistoneH2B-L2

Plasmid

#186357

Purpose

Entry clone with ORF encoding histone H2B flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

Histone H2B (H2BC11 Human)

Use

Expression of a tagged nuclear marker

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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KHBD00602

Plasmid

#39681

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG31258 (Cenp-C Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
pT7-EGFP-C1-HsDCP2

Plasmid

#25031

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DCP2 (DCP2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-CUbo-VSV

Plasmid

#212733

Purpose

Consitutive expression of CUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

CUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-ADH-CUbo-VSV

Plasmid

#212732

Purpose

Consitutive expression of CUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

CUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-CUP1-NUbo-VSV

Plasmid

#212730

Purpose

Copper-inducible expression of NUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Promoter

CUP1

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-NUbo-VSV

Plasmid

#212729

Purpose

Consitutive expression of NUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-ADH-NUbo-VSV

Plasmid

#212728

Purpose

Consitutive expression of NUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b-His6-CUbo(K63R)-GFP

Plasmid

#212722

Purpose

Bacterial expression of His6-CUbo(K63R)-GFP

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

GFP

Tags

6xHis-CUbo(K63R)

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b-His6-CUbo(K48R)-GFP

Plasmid

#212721

Purpose

Bacterial expression of His6-CUbo(K48R)-GFP

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

GFP

Tags

6xHis-CUbo(K48R)

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-TEV2-Ubc7-His6

Plasmid

#212713

Purpose

Bacterial expression of MBP-Ubc7-His6, MBP-tag cleavable with TEV-protease

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Ubc7

Tags

6xHis and MBP-TEV2

Expression

Bacterial

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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TRIPZ-CTID195-GSQ-SUMO1nc-PURO

Plasmid

#208035

Purpose

Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

SUMO1nc (SUMO1 Human)

Use

Lentiviral

Tags

Myc, C-terminal Split-TurboID

Mutation

Q94P Mutation near C-terminus suppresses cleavage…

Promoter

tetO/UBC

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-E3(48)-P2A-3xFLAG-Ubc7

Plasmid

#212804

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(48)-P2A-3xFLAG-Ubc7 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo-E3(48)-P2A-3xFLAG-Ubc7

Expression

Mammalian

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a

Plasmid

#25030

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DCP1a (DCP1A Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 23, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFPC1 MOSPD1

Plasmid

#226404

Purpose

Expression of human MOSPD1 fused to EGFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

Insert

MOSPD1 (MOSPD1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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