We narrowed to 3,388 results for: guide rna expression plasmid

1. pDECKO-mCherry MALAT1_Enhancer.3

   Plasmid

   #78538

   Purpose

   Experimental plasmid

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and U6 (for expressing sgRNA)

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDECKO-mCherry MALAT1_Exon.3

   Plasmid

   #78542

   Purpose

   Experimental plasmid

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and U6 (for expressing sgRNA)

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDECKO-mCherry MALAT1_Enhancer.2

   Plasmid

   #78537

   Purpose

   Experimental plasmid

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and U6 (for expressing sgRNA)

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Tol2_B-act_dCas9-Vp64-T2A-Citrine

   Plasmid

   #200248

   Purpose

   Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   dCas9-Vp64-T2A-Citrine
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAN057

   Plasmid

   #220053

   Purpose

   NMD-reporter plasmid that expresses a a firefly luciferase gene fused to exons 22-27 of the CFTR gene and a synthetic intron (c.3846G>A mutation; W1282X).

   Depositor

   Blake Wiedenheft

   Article

   Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

   Insert

   fLuc-CFTR(W1282X, exons 22-27) (CFTR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   c.3846G>A mutation

   Promoter

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKL2315

   Plasmid

   #187876

   Purpose

   Expresses Cre recombinase.

   Depositor

   Kan Wang

   Article

   Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

   Insert

   Cre recombinase
   

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23107

   Available since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-EGxxFP

   Plasmid

   #50716

   Purpose

   5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.

   Depositor

   Masahito Ikawa

   Article

   Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

   Insert

   split EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC19-EAMP

   Plasmid

   #138265

   Purpose

   mCherry acceptor plasmid with iCas9 target site upstream of mCherry to be used in plasmid to plasmid integration reporter assay.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   iCas9 target-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-HTLV

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CMV-TO-g0 (FLP-IN)

   Plasmid

   #236120

   Purpose

   Plasmid encoding g0 guide RNA under control of CMV promoter with two TetR binding sites, used to equalize plasmid masses for transfection

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Insert

   g0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter with two TetR binding sites

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-RSV-GFP-U6-Rosa26 gRNA (SpyCas9 scaffold)

    Plasmid

    #120296

    Purpose

    AAV vector; encodes GFP as well as a U6-driven Rosa26-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    Rosa26 gRNA (SpCas9 scaffold)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AiP942-pX330-Sncg

    Plasmid

    #181774

    Purpose

    CRISPR/Cas9 guide vector

    Depositor

    Tanya Daigle

    Insert

    Sncg guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AiP926-pX330-Batf3

    Plasmid

    #181772

    Purpose

    CRISPR/Cas9 guide vector

    Depositor

    Tanya Daigle

    Insert

    Batf3 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AiP928-pX330-Gpr139

    Plasmid

    #181767

    Purpose

    CRISPR/Cas9 guide vector

    Depositor

    Tanya Daigle

    Insert

    Gpr139 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AiP938-pX330-Slc17a7

    Plasmid

    #181775

    Purpose

    CRISPR/Cas9 guide vector

    Depositor

    Tanya Daigle

    Insert

    Slc17a7 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AiP939-pX330-Ctxn3

    Plasmid

    #181769

    Purpose

    CRISPR/Cas9 guide vector

    Depositor

    Tanya Daigle

    Insert

    Ctxn3 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS402-GPD-GFP(S65T)

    Plasmid

    #85190

    Purpose

    Integrating plasmid, GFP(S65T) under GPD promoter

    Depositor

    David Bartel

    Article

    Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

    Insert

    EGFP
    

    Use

    Integrating, shuttle vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Dec. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pH1v1

    Plasmid

    #60244

    Purpose

    gRNA expression by the H1 promoter

    Depositor

    Donald Zack

    Article

    Ranganathan et al Nat Commun. 2014 Aug 8;5:4516. doi: 10.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 promoter

    Available since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMB70

    Plasmid

    #47943

    Purpose

    Can be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro

    Plasmid

    #83306

    Purpose

    Lentivirus vector to express guideRNA and dCas9 with puro resistant gene

    Depositor

    Tohru Kimura

    Article

    Murakami et al Nat Immunol. 2021 Mar;22(3):301-311. doi

    Insert

    sgRNA and dCas9 from pX330
    

    Use

    Lentiviral

    Tags

    FLAG and PA tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for sgRNA and CBh for dCas9

    Available since

    Dec. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT040

    Plasmid

    #67640

    Purpose

    URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Insert

    single guide RNA expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pSNR52

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge