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pPOTv7-blast-BFP

Plasmid

#190258

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Sue Vaughan

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

BFP

Use

For endogenous gene tagging

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-BFP

Plasmid

#190260

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Sue Vaughan

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

BFP

Use

For endogenous gene tagging

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6.1-phleo-3Myc:mNG:3Myc

Plasmid

#181937

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-blast-3Myc:HaloTag:3Myc

Plasmid

#181943

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

HaloTag

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-g418-3Ty:mNG:3Ty

Plasmid

#179800

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-blast-3Myc:mNG:3Myc

Plasmid

#179801

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDO068

Plasmid

#198788

Purpose

Drives specific expression in the BAG neurons

Depositor

Paul Sternberg

Publication

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pgcy-33-GAL4-SK(DBD)-VP64

Expression

Worm

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pJL065

Plasmid

#198784

Purpose

Drives specific expression in the AWA neurons

Depositor

Paul Sternberg

Publication

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Podr-10-GAL4-SK(DBD)-VP64

Expression

Worm

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP061

Plasmid

#198775

Purpose

Drives specific expression in the ASE neurons

Depositor

Paul Sternberg

Publication

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pgcy-5-GAL4-SK(DBD)-VP64

Expression

Worm

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP018

Plasmid

#198766

Purpose

Drives specific expression in the ADE, CEP, and PDE neurons

Depositor

Paul Sternberg

Publication

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pdat-1-GAL4-SK(DBD)-VP64

Expression

Worm

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_VPR_mcherry

Plasmid

#167886

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-VPR

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only
NAX_CAGGS_CAS9_m4_KRAB_mcherry

Plasmid

#167878

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only
pAAV-VTKD4-ChR2-YFP

Plasmid

#178721

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of ChR2-YFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Publication

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

ChR2-YFP

Use

AAV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS5-ChR2-YFP

Plasmid

#178725

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of ChR2-YFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Publication

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

ChR2-YFP

Use

AAV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_VPR_Puro

Plasmid

#167887

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-VPR

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_VPR_Hygro

Plasmid

#167883

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-VPR

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_VPR_EYFP

Plasmid

#167882

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-VPR

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB_EBFP2

Plasmid

#167873

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTD75

Plasmid

#172899

Purpose

Hypodermal promoter col-10P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi4348 site on LGI

Depositor

David Reiner

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

TIR-1 (TIR1 Mustard Weed)

Use

CRISPR

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pUHD-SB-mAID/Zeo

Plasmid

#171681

Purpose

TRE-controlled mAID-fusion sleeping beauty transposon vector with selection marker (zeocin)

Depositor

Randy Poon

Publication

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Type

Empty backbone

Tags

mAID

Expression

Mammalian

Promoter

TRE

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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