Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 17,384 results for: puromycin

Showing: 3001 - 3020 of 17384 results

Results per page:

Sort results by:

sgOPTI:U6-gRNA-CS1-puroR

Plasmid

#256351

Purpose

lenti virus vector expressing a Cas9 guide RNA under a human U6 promoter. Contains capture sequence 1 (CS1) in the Cas9 scaffold. Contains puromycin resistance cassette.

Depositor

Jesse Engreitz

Article

Ray et al bioRxiv [Preprint]. 2025 Sep 17:2025.09.

Insert

sgRNA

Use

Lentiviral

Promoter

U6 promoter

Available Since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-Puro-EF1A-HA-PARP1-3xGS-TurboC-T2A-XRCC1-myc-3xGS-TurboN

Plasmid

#249504

Purpose

PARP1 and XRCC1 Split TurboID with a puromycin resistance cassette

Depositor

Robert Sobol

Article

Ibrahim et al NAR Cancer. 2025 Dec 31;7(4):zcaf057. do

Inserts

PARP1 (PARP1 Human)
XRCC1 (XRCC1 Human)

Use

Lentiviral

Tags

HA, Myc, Split TurboID: C, and Split TurboID: N

Expression

Mammalian

Available Since

Jan. 22, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-puro-EF1A-3XFLAG-hASB8_G198R

Plasmid

#242465

Purpose

Expresses human ASB8 protein with an N-terminal 3xFLAG tag along with a puromycin selection marker. The G198R mutation disrupts XPO1 binding.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

ASB8 (ASB8 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

G198R

Promoter

EF1A

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-puro-EF1A-3XFLAG-hASB8_L199R

Plasmid

#242466

Purpose

Expresses human ASB8 protein with an N-terminal 3xFLAG tag along with a puromycin selection marker. The L199R mutation disrupts XPO1 binding.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

ASB8 (ASB8 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

L199R

Promoter

EF1A

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-puro-EF1A-3XFLAG-hASB8_L199G

Plasmid

#242467

Purpose

Expresses human ASB8 protein with an N-terminal 3xFLAG tag along with a puromycin selection marker. The L199G mutation disrupts XPO1 binding.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

ASB8 (ASB8 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

L199G

Promoter

EF1A

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-puro-EF1A-3XFLAG-hASB8_ANK3-4

Plasmid

#242468

Purpose

Expresses human ASB8 protein with an N-terminal 3xFLAG tag along with a puromycin selection marker. The ANK3-4 mutations disrupt XPO1 binding.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

ASB8 (ASB8 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

ANK3-4 = R87A - E95A - K96A - W124G - K128G

Promoter

EF1A

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-puro-EF1A-Tag100-hASB8_G198R

Plasmid

#242461

Purpose

Expresses human ASB8 protein with an N-terminal Tag100 along with a puromycin selection marker. The G198R mutation disrupts XPO1 binding.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

ASB8 (ASB8 Human)

Use

Lentiviral

Tags

Tag100

Expression

Mammalian

Mutation

G198R

Promoter

EF1A

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pvPE-V3-Puro

Plasmid

#240289

Purpose

Encodes third generation porcine endogenous retrovirus-derived prime editing (pvPE) system with puromycin resistance gene in to mammalian cells for gene editing.

Depositor

Lusheng Huang

Article

Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779

Insert

pvPE-V3-Puro

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pB-Puro-DEST (JDW 925)

Plasmid

#229808

Purpose

A puromycin selectable, PiggyBac destination vector compatible with 4-way multi-site gateway cloning system

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLCHKOv3-mClover3

Plasmid

#209034

Purpose

Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites, mClover3 and puromycin selection marker

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FLAG-2xSTREP-CCND1(P287A)-Puro

Plasmid

#172641

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1(P287A) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Pro287Ala

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FLAG-2xSTREP-CCND1(T286A)-Puro

Plasmid

#172642

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1(T286A) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Thr286Ala

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-2xFLAG-2xSTREP-CCND1(T286A)-Puro

Plasmid

#172633

Purpose

Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286A) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Thr286Ala

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-2xFLAG-2xSTREP-CCND1-Puro

Plasmid

#172634

Purpose

Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FLAG-2xSTREP-CCND3(P284L)-Puro

Plasmid

#172617

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(P284L) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Pro284Leu

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FLAG-2xSTREP-CCND2(P281L)-Puro

Plasmid

#172624

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2(P281L) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Pro281Leu

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-PGK-flag-RAP1B-N17-puro

Plasmid

#156169

Purpose

Lentiviral vector for expression of flag-RAP1B-N17, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1B (RAP1B Bovine)

Use

Lentiviral

Tags

2xFLAG

Expression

Mammalian

Mutation

changed Serine 17 to Asparagine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-PGK-RAP2A-WT-puro

Plasmid

#156170

Purpose

Lentiviral vector for expression of RAP2A-WT, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Expression

Mammalian

Mutation

wild type form

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-PGK-RAP2A-V12-puro

Plasmid

#156171

Purpose

Lentiviral vector for expression of RAP2A-V12, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Expression

Mammalian

Mutation

changed Glycine 12 to Valine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-PGK-RAP2A-N17-puro

Plasmid

#156172

Purpose

Lentiviral vector for expression of RAP2A-N17, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

changed Serine 17 to Asparagine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 3001 - 3020 of 17384 results

Results per page:

Sort results by: