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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 70,693 results for: LAS

Showing: 4531 - 4560 of 70693 results

  6. pMflT-o4-mNG

    Plasmid
    #201855
    Purpose
    Mesoplasma florum oriC plasmid expressing mNeonGreen fluorescent protein
    Depositor
    Sébastien Rodrigue
    Insert
    mNeonGreen
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    201855-plasmid-map-sequence-id-409945

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  7. pMflT-o4-YFP

    Plasmid
    #201856
    Purpose
    Mesoplasma florum oriC plasmid expressing LanYFP fluorescent protein
    Depositor
    Sébastien Rodrigue
    Insert
    LanYFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    201856-plasmid-map-sequence-id-396316

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  9. pSc1

    Plasmid
    #80436
    Purpose
    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell
    Depositor
    Ervin Welker
    Article
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Inserts
    sp Cas9 gRNA
    eGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6
    Available since
    Aug. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80436-plasmid-map-sequence-id-151445

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  10. pET His6 MBP TEV HDAC8_374

    Plasmid
    #122174
    Purpose
    Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag
    Depositor
    David Christianson
    Article
    Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22
    Insert
    His6 MBP TEV HDAC8_374 (HDAC8 Human)
    Use
    Tags
    hexahistidine tag and tobacco etch virus protease…
    Expression
    Bacterial
    Mutation
    Residues Met1–Pro7 and H375-V377 have been delete…
    Promoter
    T7
    Available since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    122174-plasmid-map-sequence-id-237469

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  11. pSc1-puro

    Plasmid
    #80438
    Purpose
    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycin
    Depositor
    Ervin Welker
    Article
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Inserts
    sp Cas9 gRNA
    eGFP
    Puromycin resistance
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, U6, and hPGK
    Available since
    Sept. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    80438-plasmid-map-sequence-id-151453

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  12. MIGR1-PREP1

    Plasmid
    #78147
    Purpose
    retroviral expression of PREP1 and GFP
    Depositor
    Francesco Blasi
    Insert
    PREP1 (PKNOX1 Human)
    Use
    Retroviral
    Tags
    IRES-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. MIGR1-Meis1a

    Plasmid
    #78148
    Purpose
    retroviral expression of Meis1a and GFP
    Depositor
    Francesco Blasi
    Insert
    Meis1a (Meis1 Mouse)
    Use
    Retroviral
    Tags
    IRES-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  16. pGU08

    Plasmid
    #212149
    Purpose
    Plasmid expressing catalase-peroxidase and D-amino acid dehydrogenase under constitutive promoter PgyrA control. Best E. coli glycine utilization plasmid.
    Depositor
    Kang Zhou
    Insert
    catalase-peroxidase, D-amino acid dehydrogenase (katG katG: E. coli, dadA6: Cupriavidus necator)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PgyrA
    Available since
    March 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    212149-plasmid-map-sequence-id-424775

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  17. INT_Cargo_PgyrA_katG_dadA6

    Plasmid
    #212142
    Purpose
    Cargo plasmid for integrating PgyrA expression cassette of catalase-peroxidase and D-amino acid dehydrogenase into the genome. Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Insert
    catalase-peroxidase, D-amino acid dehydrogenase (katG katG: E. coli, dadA6: Cupriavidus necator)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PgyrA
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212142-plasmid-map-sequence-id-420769

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  18. mCerulean3-Bik-s2193

    Plasmid
    #177425
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bik, in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    mCerulean3
    Expression
    Mammalian
    Mutation
    Promoter
    Human ferritin
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177425-plasmid-map-sequence-id-347947

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  19. dSaCas9-KRAB

    Plasmid
    #205250
    Purpose
    In vitro transcription plasmid for production of dSaCas9-KOX1 KRAB mRNA for transcriptional activation (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #106219.
    Depositor
    Rasmus Bak
    Insert
    dSaCas9-KRAB
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    205250-plasmid-map-sequence-id-409107

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  23. pHelper_O

    Plasmid
    #173783
    Purpose
    The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.
    Depositor
    Keith Shearwin
    Article
    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102
    Insert
    186 Integrase
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    E59G - please see depositor comments
    Promoter
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    173783-plasmid-map-sequence-id-342367

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  30. pTrEno

    Plasmid
    #115474
    Purpose
    Eno-promoter driven expression vector for protein production in Trichoderma reesei
    Depositor
    Steve Decker
    Article
    Linger et al Biotechnol Biofuels. 2015 Mar 18;8:45. d
    Insert
    Cel7A
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Introns have been removed
    Promoter
    Trichoderma reesei Enolase (Eno) promoter
    Available since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    115474-plasmid-map-sequence-id-225594

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Showing: 4531 - 4560 of 70693 results