We narrowed to 9,256 results for: SUB;

1. pCMV_PML1 or 2-FLAG-YFast-TEV-6xHis-CylLL S15T-P2A-Myc-CylM

   Plasmid

   #225061

   Purpose

   Expression and Plasma membrane-localization of CylM-modified CylLL-S15T in human cells

   Depositor

   Wilfred van der Donk

   Article

   Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

   Insert

   CylLL-S15T
   

   Use

   Tags

   Multiple tags due to polycistronic expression

   Expression

   Mammalian

   Mutation

   S15T

   Promoter

   CMV

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV_Myc-CylM-P2A-FLAG-YFast-TEV-His-CylLL-S15T

   Plasmid

   #225063

   Purpose

   Expression of CylM-modified CylLL-S15T in human cells

   Depositor

   Wilfred van der Donk

   Article

   Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

   Insert

   CylLL-S15T
   

   Use

   Tags

   Multiple tags due to polycistronic expression

   Expression

   Mammalian

   Mutation

   S15T

   Promoter

   CMV

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-AP-SpyCatcher

   Plasmid

   #223636

   Purpose

   Protein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coli

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   SpyCatcher003 S49C
   

   Use

   Tags

   His10, AP fragment of split APEX2 tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-SpyCatcher -AVI

   Plasmid

   #223635

   Purpose

   Protein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coli

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   SpyCatcher003 S49C
   

   Use

   Tags

   AVI tag and His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGM258

   Plasmid

   #220176

   Purpose

   Expresses exogenous C-terminal mutant TTC1 (I271D)

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   TTC1 (TTC1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   I271D

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGM133

   Plasmid

   #220173

   Purpose

   CRISRPi guide RNA against TTC1

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   TTC1 (sgRNA) (TTC1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Xlone Puro SNAP-eGFP-BASU

   Plasmid

   #218452

   Purpose

   expresses the SNAP-eGFP-BASU fusion protein via stable PiggyBac integration

   Depositor

   Thorsten Stafforst

   Article

   Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

   Insert

   SNAP-eGFP-BASU
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5_NLS-Halo-eGFP-BASU

   Plasmid

   #218449

   Purpose

   expressed the NLS-Halo-eGFP-BASU fusion protein via stable Flp-In integration

   Depositor

   Thorsten Stafforst

   Article

   Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

   Insert

   NLS-Halo-eGFP-BASU
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPRISM-QF2-he1.1:YFP

   Plasmid

   #184819

   Purpose

   Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.

   Depositor

   Marnie Halpern

   Article

   Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

   Insert

   QF2-pA-he1.1:YFP
   

   Use

   CRISPR; Backbone for cloning of homology arms (fo…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPRISM-QF2-he1.1:YFP-rln3a-HA

    Plasmid

    #184820

    Purpose

    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    rln3a-HA
    

    Use

    CRISPR; For integration of qf2 by homology-direct…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TOPO-nmba

    Plasmid

    #184821

    Purpose

    Used to synthesize RNA probe for in situ hybridization for nmba

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    nmba (nmba Zebrafish)
    

    Use

    Template for synthesis of nmba probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGM268

    Plasmid

    #220181

    Purpose

    Expresses exogenous C-terminal mutant TTC1 (I271D) with an N-terminal sfGFP tag

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    TTC1 (TTC1 Human)
    

    Use

    Lentiviral

    Tags

    sfGFP

    Expression

    Mutation

    I271D

    Promoter

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP-C1-IFT22

    Plasmid

    #218720

    Purpose

    Expresses N-terminally EGFP-tagged IFT22 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

    Insert

    IFT22 (RABL5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGM265

    Plasmid

    #220179

    Purpose

    Expresses exogenous wild-type TTC1 with an N-terminal sfGFP tag

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    TTC1 (TTC1 Human)
    

    Use

    Lentiviral

    Tags

    sfGFP

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGM216

    Plasmid

    #220175

    Purpose

    Expresses exogenous TPR mutant TTC1 (R190A_R194A)

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    TTC1 (TTC1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    R190A + R194A

    Promoter

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SUMO CRYGS 16-177 Q16C

    Plasmid

    #217673

    Purpose

    N-terminally truncated human gamma S crystallin residues 16-177 with Q16C mutation for native chemical ligation

    Depositor

    Larry David

    Insert

    CRYGS (CRYGS Human)
    

    Use

    Tags

    6XHis and SUMO

    Expression

    Bacterial

    Mutation

    Glutamine 16 changed to Cysteine, and amino acids…

    Promoter

    T7

    Available since

    May 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMx/ATP8B4-HA

    Plasmid

    #209229

    Purpose

    Mammalian expression of ATP8B4

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

    Insert

    ATP8B4 (ATP8B4 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMx/ATP8B3-HA

    Plasmid

    #209228

    Purpose

    Mammalian expression of ATP8B3

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

    Insert

    ATP8B3 (ATP8B3 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQE60 G121A wgMDH

    Plasmid

    #204252

    Purpose

    Bacterial expression of G121A wgMDH with IPTG induction - Active site loop mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    G121A

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQE60 R196A wgMDH

    Plasmid

    #204271

    Purpose

    Bacterial expression of R196A wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R196A

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge