We narrowed to 11,414 results for: Vars;

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pCMV-UGI-nCas9(H840A)-GSVG

Plasmid

#187411

Purpose

DddA GSVG variant

Depositor

Jin-Soo Kim

Article

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Insert

DddA GSVG variant

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)

Plasmid

#115698

Purpose

ER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiae

Depositor

Eckhard Boles

Article

Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

Insert

SSKar2-sfpHluorin-HDEL

Tags

ER retention signal containing HDEL and ER signal…

Expression

Yeast

Mutation

for introduced mutations see publication

Promoter

MET25

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-mIFNa2

Plasmid

#135098

Purpose

Protein expression of mouse IFNa2 in mammalian cells

Depositor

Pablo Penaloza-MacMaster

Article

Palacio et al J Exp Med. 2020 Dec 7;217(12). pii: 1520

Insert

mIFN-α2 (Ifna2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 mSmoothened WT

Plasmid

#126408

Purpose

Full length mouse Smoothened cDNA. Expression of Smoothened in mammalian cell lines.

Depositor

Stacey Ogden

Article

Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

Insert

m-Smoothened (Smo Mouse)

Expression

Mammalian

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-TadCDd-BlastR

Plasmid

#202558

Purpose

Mammalian expression of evoCjCas9 TadCDd

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-TadCDd

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAB078d5

Plasmid

#79204

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ppro1 434cI

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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p416 72Q GPD

Plasmid

#1179

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 72Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
PKAcs-GGSD5X-GFP

Plasmid

#108584

Purpose

Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

Depositor

Robert Hughes

Article

Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

Insert

protein kinase A, catalytic subunit

Tags

GFP

Expression

Mammalian

Mutation

H87Q and W196R

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDJSVT86

Plasmid

#108965

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tag

Depositor

Daniel Slade

Article

Casasanta et al J Biol Chem. 2017 Dec 8;292(49):20240-20

Type

Empty backbone

Tags

OmpA 1-27, 6His

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT-PonA-BI-Gl-WT-24xMS2-WT-18xPP7 (WW)

Plasmid

#189791

Purpose

Express wild-type beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cells

Depositor

Robert Singer

Article

Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

Insert

beta-globin (HBB Mouse, Human)

Expression

Mammalian

Promoter

Ponasterone A inducible

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCFJ2092 - [1-2] ENTR - Fluor - mNeon(PATC)

Plasmid

#159853

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mNeon(PATC)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-R4-uAb

Plasmid

#101800

Purpose

Mammalian cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

Depositor

Matthew DeLisa

Article

Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

Insert

R4-uAb

Tags

6xHis and Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET-DUET-1-mRhubarb713-HO

Plasmid

#141199

Purpose

Expresses mRhubarb713 fluorescent protein and heme oxygenase

Depositor

Elad Firnberg

Article

Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

Inserts

mRhubarb713
heme oxygenase

Tags

6x-His

Expression

Bacterial

Mutation

R134H, W309R, Q310A compared to iRFP

Promoter

T7

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a(+)- PC-MjMAT

Plasmid

#179372

Purpose

PC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.

Depositor

Andrea Rentmeister

Article

Peters et al Chembiochem. 2021 Oct 4. doi: 10.1002/cb

Insert

PC-MjMAT

Tags

6xHis

Expression

Bacterial

Mutation

L147A, I351A

Promoter

T7

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-BE4max-BlastR

Plasmid

#194061

Purpose

Mammalian expression of evoCjCas9 CBE4max

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-BE4max

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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p416 103Q GAL

Plasmid

#1186

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 103Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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epB_UbC_tdMS2cp-tagRFP-Tx2

Plasmid

#119908

Purpose

ePiggyBac mammalian expression plasmid for tandem dimer MS2cp-tagRFP-T fusion

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

HA-tdMS2cp-tagRFP-Tx2-nls

Expression

Mammalian

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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CP1028-CBEmax

Plasmid

#119802

Purpose

C-to-T base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

rApobec1-nSpCas9 (CP1028) -UGI-UGI

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#68

Plasmid

#153527

Purpose

Expression of the anti SARS-CoV-2_RBD sybody Sb#68

Depositor

Markus Seeger

Article

Walter et al bioRxiv 2020.04.16.045419

Insert

Sb#68

Expression

Bacterial

Promoter

pBAD

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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p416 25Q GAL

Plasmid

#1185

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 25Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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