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We narrowed to 63,680 results for: cloning

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Showing: 3021 - 3040 of 63680 results

  1. SIVu

    Plasmid
    #81089
    Purpose
    Single Integration Vector URA - Cloning vector for single integration into the URA3 locus of S.cerevisiae containing two MCS.
    Depositor
    Serge Pelet
    Publication
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Type
    Empty backbone
    Use
    Cre/Lox
    Expression
    Yeast
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    81089-plasmid-map-sequence-id-164362

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  3. Cas9/pTREX-n

    Plasmid
    #68708
    Purpose
    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.
    Depositor
    Roberto Docampo
    Publication
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    Use
    CRISPR
    Tags
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …
    Mutation
    C4239T mutation that eliminates a BamHI restricti…
    Available since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68708-plasmid-map-sequence-id-123989

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  6. pLSB-HYG

    Plasmid
    #166699
    Purpose
    Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Publication
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166699-plasmid-map-sequence-id-474294

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  7. pFB-LIC-Bse

    Plasmid
    #26108
    Purpose
    SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection
    Depositor
    Opher Gileadi
    Publication
    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    His6 - TEV
    Expression
    Insect
    Promoter
    polyhedrin
    Available since
    Oct. 4, 2010
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    26108-plasmid-map-sequence-id-12260

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  12. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid
    #104909
    Purpose
    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    June 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    104909-plasmid-map-sequence-id-206071

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  18. DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)

    Plasmid
    #90086
    Purpose
    This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Publication
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90086-plasmid-map-sequence-id-182687

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Showing: 3021 - 3040 of 63680 results