We narrowed to 4,135 results for: pCMV mammalian

1. pEJS2714-CMV-iNme2Smu-ABE8e-i1-L10

   Plasmid

   #228735

   Purpose

   Expresses domain inlaid iNme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid iNme2Smu nCas9 (D16A)-i1-L10
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEJS2713-CMV-iNme2-ABE8e-i1-L10

   Plasmid

   #228734

   Purpose

   Expresses domain inlaid iNme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid iNme2 nCas9 (D16A)-i1-L10
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. xCas9 3.6

   Plasmid

   #108384

   Purpose

   Mammalian expression vector for xCas9 3.6

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9 3.6
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Equalizer-L plasmid

   Plasmid

   #169732

   Purpose

   Equalizer variant plasmid with low eGFP output levels but the best gene dosage compensation capability.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   pCMV-tetO2

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF-ABEmax

   Plasmid

   #164415

   Purpose

   EF1alpha promoter + ABEmax (derived from #112095). Mammalian cell adenosine base editor expression vector.

   Depositor

   Xiao Wang

   Article

   Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

   Insert

   ABEmax
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Equalizer-H plasmid

   Plasmid

   #169367

   Purpose

   Equalizer variant plasmid with high eGFP output levels but reduced gene dosage compensation capability.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   tetR-P2A-eGFP-miR(FF3) target-miR(FF3)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   pCMV-tetO2

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Equalizer-M plasmid

   Plasmid

   #169731

   Purpose

   Equalizer variant plasmid with medium eGFP output levels and medium gene dosage compensation capability.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   pCMV-tetO2

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PE_CO

   Plasmid

   #190335

   Purpose

   Prime editor (PE2) with codon-optimized Reverse transcriptase for prime editing in mammalian cells

   Depositor

   Rasmus Bak

   Article

   Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

   Insert

   PE2 with codon-optimized reverse transcriptase
   

   Expression

   Mammalian

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. evoBxb1

   Plasmid

   #222338

   Purpose

   Plasmid for expressing evolved Bxb1 in the mammalian cells

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   Codon-optimized evolved Bxb1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA

    Plasmid

    #176270

    Purpose

    Expression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cells

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    TadA-TadA*(8e)-dCasMINI-V4
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64

    Plasmid

    #169597

    Purpose

    Tier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    dead S.pyogenes Cas9 - VP64 fusion
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST-FLAG

    Plasmid

    #183511

    Purpose

    Gateway converted, destination vector with N-terminal Flag tag. For mammalian expression.

    Depositor

    Emanuele Panza

    Article

    Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

    Type

    Empty backbone

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-CBLB

    Plasmid

    #192082

    Purpose

    Mammalian expression of human CBLB with FLAG tag

    Depositor

    Patrick Concannon

    Article

    Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

    Insert

    CBLB (CBLB Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. SaKKH-ABEmax

    Plasmid

    #119815

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSaCas9 (KKH)
    

    Expression

    Mammalian

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. VRQR-ABEmax

    Plasmid

    #119811

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (VRQR)
    

    Expression

    Mammalian

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Galt1-Grx1-roGFP1-iE

    Plasmid

    #230029

    Purpose

    Expressing Grx1-roGFP1-iE fluorescent fusion protein in mammalian cells with localization in the Golgi apparatus.

    Depositor

    Pablo Rivera-Fuentes

    Insert

    Galt1-Grx1-roGFP1-iE (B4GALT1 Synthetic, Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. ND4.2-Right DdCBE-G1397-C- T1413I-GFP

    Plasmid

    #179686

    Purpose

    Expression of base editor in mammalian cells and for cell sorting

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_P2A_GFP_ATP5B 3'UTR
    

    Expression

    Mammalian

    Mutation

    T1413I

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC0078 RESCUE

    Plasmid

    #130661

    Purpose

    pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

    Insert

    dRanCas13b-ADAR2dd (RESCUE)
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. TDP-43 mRuby

    Plasmid

    #216225

    Purpose

    TDP-43 with a C-terminal mRuby tag for expression in mammalian cells.

    Depositor

    Jennifer Lee

    Article

    Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    mRuby

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. TL1 DdCBE A1 (DddA6-C)

    Plasmid

    #221992

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: T1413I

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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