We narrowed to 5,828 results for: pcas

1. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-gs-UGI-NLS(SV40)(AsBE1.4) (RTW1008)

   Plasmid

   #114080

   Purpose

   CAG promoter expression plasmid for human codon optimized AsBE1.4

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.4)
   

   Tags

   2x SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(AsBE1.1) (RTW1351)

   Plasmid

   #114079

   Purpose

   CAG promoter expression plasmid for human codon optimized AsBE1.1

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1)
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.3) (RTW1296)

   Plasmid

   #114083

   Purpose

   CAG promoter expression plasmid for human codon optimized enAsBE1.3

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.3)
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548R and D908A, human codon optimi…

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFS_1113_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader-ARRAY2-DR2-FaqI_Term_NotI

   Plasmid

   #184741

   Purpose

   encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23103

   Depositor

   Randall Platt

   Article

   Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

   Insert

   FsRT-Cas1(opt)-Cas2(opt)
   

   Expression

   Bacterial

   Mutation

   sequence codon optimized for expression in E. coli

   Promoter

   pTetA

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFS_1142_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader1-ARRAY1-DR1-FaqI_Term_NotI

   Plasmid

   #184742

   Purpose

   encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 1 transcribed by BBa_J23103

   Depositor

   Randall Platt

   Article

   Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

   Insert

   FsRT-Cas1(opt)-Cas2(opt)
   

   Expression

   Bacterial

   Mutation

   sequence codon optimized for expression in E. coli

   Promoter

   pTetA

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFS_1064_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23106-Leader-ARRAY2-DR2-FaqI_Term_NotI

   Plasmid

   #184739

   Purpose

   encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23106

   Depositor

   Randall Platt

   Article

   Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

   Insert

   FsRT-Cas1(opt)-Cas2(opt)
   

   Expression

   Bacterial

   Mutation

   sequence codon optimized for expression in E. coli

   Promoter

   pTetA

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFS_1112_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23109-Leader-ARRAY2-DR2-FaqI_Term_NotI

   Plasmid

   #184740

   Purpose

   encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23109

   Depositor

   Randall Platt

   Article

   Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

   Insert

   FsRT-Cas1(opt)-Cas2(opt)
   

   Expression

   Bacterial

   Mutation

   sequence codon optimized for expression in E. coli

   Promoter

   pTetA

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting

   Plasmid

   #92139

   Purpose

   Targeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selection

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   chromatin licensing and DNA replication factor 1 (GMNN, CDT1 Human)
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFS_0414_pET30_MCS-BBaJ23100-LuxR-B0015term-pLux rR-Fluc-rrnBterm-pCat-tetR-Term-ptetA-Rluc-rrnBterm_FsRT-Cas1-Cas2_ARRAY2_FaqI

   Plasmid

   #117005

   Purpose

   Expresses FsRT-Cas1-Cas2 under pT7lac, Fluc under pLuxR and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Fusicatenibacter saccharivorans RT-Cas1-Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)

    Plasmid

    #114084

    Purpose

    CAG promoter expression plasmid for human codon optimized enAsBE1.4

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4)
    

    Tags

    2x SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A, human codon optimi…

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352)

    Plasmid

    #114081

    Purpose

    CAG promoter expression plasmid for human codon optimized enAsBE1.1

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1)
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A, human codon optimi…

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFS_1061_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_GGC(BbsI)-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid

    #184738

    Purpose

    encodes aTc inducible FsRT-Cas1–Cas2 and transcriptionally insulated FsCRISPR Array 2

    Depositor

    Randall Platt

    Article

    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

    Insert

    FsRT-Cas1(opt)-Cas2(opt)
    

    Expression

    Bacterial

    Mutation

    sequence codon optimized for expression in E. coli

    Promoter

    pTetA

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)

    Plasmid

    #114082

    Purpose

    CAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2)
    

    Tags

    2x SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A, human codon optimi…

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST-DHFR F[3]-C (LEU2)

    Plasmid

    #177796

    Purpose

    Gateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

    Type

    Empty backbone

    Tags

    DHFR F[3]

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST-DHFR F[1,2]-C (TRP1)

    Plasmid

    #177795

    Purpose

    Gateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

    Type

    Empty backbone

    Tags

    DHFR F[1,2]

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST-DHFR F[3]-N (LEU2)

    Plasmid

    #177798

    Purpose

    Gateway destination vector to insert genes of interest having a N-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST-DHFR F[1,2]-N (TRP1)

    Plasmid

    #177797

    Purpose

    Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

    Type

    Empty backbone

    Tags

    DHFR F[1,2]

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJBL6931

    Plasmid

    #217369

    Purpose

    sfGFP reporter for WT Pca rhtB ZTP riboswitch

    Depositor

    Julius Lucks

    Article

    Bushhouse et al bioRxiv 2024.03.29.587317

    Insert

    Pca rhtB riboswitch sfGFP reporter
    

    Available Since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJBL7346

    Plasmid

    #217370

    Purpose

    sfGFP reporter for synthetic transcriptional variant of Pca rhtB ZTP riboswitch

    Depositor

    Julius Lucks

    Article

    Bushhouse et al bioRxiv 2024.03.29.587317

    Insert

    Pca rhtB synthetic transcriptional riboswitch sfGFP reporter
    

    Available Since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJBL7316

    Plasmid

    #217371

    Purpose

    sfGFP reporter for chimeric riboswitch with Cbe pfl ZTP riboswitch aptamer and transcriptional variant of Pca rhtB ZTP riboswitch expression platform

    Depositor

    Julius Lucks

    Article

    Bushhouse et al bioRxiv 2024.03.29.587317

    Insert

    Chimeric ZTP riboswitch with Cbe pfl aptamer and transcriptional variant of Pca rhtB expression platform sfGFP reporter
    

    Available Since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge