Addgene

Showing: 3021 - 3040 of 11705 results

Results per page:

Sort results by:

pcDNA HA-Gnat1 WT-3UTR

Plasmid

#184026

Purpose

Expresses mouse Gnat1 protein with N-terminal HA tag from cDNA that contains the full length 3'-UTR

Depositor

Peter Stoilov

Article

Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

Insert

Gnat1 (Gnat1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-hMcl-1(S159A)

Plasmid

#25374

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

hMCL1(S159A) (MCL1 Human)

Use

Tags

V5 and His tag

Expression

Mammalian

Mutation

changed serine 159 to alanine

Promoter

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA4-FKBP-LAP1

Plasmid

#34841

Depositor

Alice Ting

Article

Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

Insert

FKBP-LAP1

Use

Tags

His Tag, T7 tag, and myc

Expression

Mutation

Promoter

CMV

Available since

Feb. 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5/FRT/TO_CEP41_HaloTag9

Plasmid

#169331

Purpose

Expression of HaloTag9 located at the microtubules of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CEP41-HaloTag9 (CEP41 Human)

Use

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.4_7C11-1 light chain

Plasmid

#198252

Purpose

recombinant expression of light chain of monoclonal anti-AMP antibody 7C11-1

Depositor

Aymelt Itzen

Article

Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

Insert

light chain of monoclonal anti-AMP antibody 7C11-1 (mouse kappa)

Use

Tags

IL10 signal peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.4_7C11-1 heavy chain

Plasmid

#198251

Purpose

recombinant expression of heavy chain of monoclonal anti-AMP antibody 7C11-1

Depositor

Aymelt Itzen

Article

Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

Insert

heavy chain of monoclonal anti-AMP antibody 7C11-1 (mouse IgG2a)

Use

Tags

IL10 signal peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LHP334-pcDNA3-STE2

Plasmid

#32158

Depositor

Linda Hicke

Insert

STE2 (STE2 Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
ITGB2_pcDNA6.2/EmGFP-Bsd

Plasmid

#176983

Purpose

Mammalian expression vector encoding ITGB2 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

ITGB2 (ITGB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-VenusFlag-hTICAM1

Plasmid

#197631

Purpose

Human TRIF protein expression in mammalian cells

Depositor

Haian Fu

Insert

TICAM1 (TICAM1 Human)

Use

Tags

Venus-Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pY126 (pcDNA3.1-huBoCpf1)

Plasmid

#92302

Purpose

Expression of humanized BoCpf1

Depositor

Feng Zhang

Insert

huBoCpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pY113 (pcDNA3.1-huPb2Cpf1)

Plasmid

#92272

Purpose

Expression of humanized Pb2Cpf1

Depositor

Feng Zhang

Insert

huPb2Cpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-UL87-2xStrep

Plasmid

#138434

Purpose

Expresses full-length UL87 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

UL87 (UL87 HCMV (HHV-5))

Use

Tags

Strep

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 FLIPE-10u

Plasmid

#13542

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207R

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207R in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)_Granulin 4+linker5

Plasmid

#176924

Purpose

express Granulin 4 with linker 5 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 4+linker5 (GRN Human)

Use

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-sACE2v2-Fc(IgA1)

Plasmid

#145157

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 2

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Fc region of human IgA1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNAI Tat C30G HA (P#38)

Plasmid

#14656

Depositor

Matija Peterlin

Article

Cujec et al Mol Cell Biol. 1997 Apr . 17(4):1817-23.

Insert

Tat (tat HIV)

Use

Tags

HA

Expression

Mammalian

Mutation

C30G C34G

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)mGAT1-0-YFP

Plasmid

#41663

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Use

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; C-terminal hyd…

Promoter

CMV

Available since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-Nsp1-HF

Plasmid

#157681

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

HF-Nsp1 (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCDNA3.0_LynSNAP IRES EGFP

Plasmid

#136625

Purpose

Plasmid encoding outer inner membrane localization of SNAP-Tag and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag (IRES EGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-mRFP-Syx1

Plasmid

#35707

Depositor

Arie Horowitz

Article

Liu et al Mol Biol Cell. 2006 Apr;17(4):1880-7. Ep

Insert

Syx1 (Plekhg5 Mouse)

Use

Tags

mRFP

Expression

Mammalian

Mutation

R205K

Promoter

CMV

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only