We narrowed to 11,993 results for: pcdna

1. pcDNA3.1(+)_Paragranulin-no linker

   Plasmid

   #176917

   Purpose

   express Paragranulin without linker 1 in mammalian cells

   Depositor

   Thomas Kukar

   Article

   Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

   Insert

   Paragranulin (GRN Human)
   

   Use

   Tags

   Twin-Strep and FLAG tags encoded after Signal Pep…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA6-N-3XFLAG-Fzd6

   Plasmid

   #123588

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   frizzled class receptor 6 (Fzd6 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-nAChr-Alpha6

   Plasmid

   #48812

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr-alpha6 (Chrna6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.3-hALDOA D34S

   Plasmid

   #210810

   Purpose

   To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

   Depositor

   Sheng-cai Lin

   Article

   Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

   Insert

   ALDOA (ALDOA Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   residues 34 of ALDOA mutated to serine

   Promoter

   CMV

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-Cx43-YA/VD-GFP

   Plasmid

   #60703

   Purpose

   mammalian expression of rat Cx43 YA/VD mutant fused to GFP

   Depositor

   Parmender Mehta

   Article

   Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

   Insert

   Cx43 (Gja1 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   YA/VD (Y286A/V289D)

   Promoter

   CMV

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCDNA3.1-hMcl-1(T163A)

   Plasmid

   #25376

   Depositor

   Roger Davis

   Article

   Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

   Insert

   hMCL1 (MCL1 Human)
   

   Use

   Tags

   V5 and His tag

   Expression

   Mammalian

   Mutation

   changed threonine 163 to alanine

   Promoter

   Available Since

   Aug. 23, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA-Cx43-Myc

   Plasmid

   #60693

   Purpose

   mammalian expression of Myc-tagged rat Cx43

   Depositor

   Parmender Mehta

   Article

   Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

   Insert

   Cx43 (Gja1 Rat)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1: TfR1-CoilE

   Plasmid

   #181988

   Purpose

   CoilE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with CoilR labeled with a reporter

   Depositor

   Kimberly Beatty

   Article

   Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

   Insert

   TfR1-CoilE
   

   Use

   Tags

   CoilE

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-LAP-KNL1-M3

   Plasmid

   #115896

   Purpose

   Used for expression of siRNA-resistant KNL1-M3 (modified codons 258 and 259) from the FRT site of FlpIn cells upon addition of doxycyclin.

   Depositor

   Geert Kops

   Article

   Vleugel et al J Cell Biol. 2013 Dec 23;203(6):943-55.

   Insert

   KNL1-M3 (KNL1 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Fragment of KNL1 containing three KNL1 repeat seq…

   Promoter

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. ciCas9(L22)_pcDNA5

    Plasmid

    #100551

    Purpose

    Expresses ciCas9(L22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

    Insert

    ciCas9(L22)
    

    Use

    CRISPR; Frt, flp-in

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA6-LRRC38-V5-His

    Plasmid

    #127820

    Purpose

    Expression of human LRRC38 in mammalian cells

    Depositor

    Jiusheng Yan

    Article

    Li et al J Gen Physiol. 2015 Jun;145(6):543-54. d

    Insert

    LRRC38 (LRRC38 Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO DNAJB6a

    Plasmid

    #19475

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB6a (DNAJB6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. N-TAP A2A in pcDNA3

    Plasmid

    #67850

    Purpose

    mammalian expression of A2A receptor with N terminal TAP tag

    Depositor

    Christian Gruber

    Article

    Bergmayr et al J Biol Chem. 2013 Oct 4;288(40):28831-44

    Insert

    adenosine A2a receptor (ADORA2A Human)
    

    Use

    Tags

    TAP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1/GS-DJ1-L166P-V5

    Plasmid

    #29343

    Depositor

    Mark Cookson

    Article

    Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Tags

    His6 and V5 epitope

    Expression

    Mammalian

    Mutation

    L166P

    Promoter

    Available Since

    March 7, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-Cerulean-WW-NES

    Plasmid

    #84629

    Purpose

    Encodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    Cerulean-WW-NES
    

    Use

    Tags

    6xHis, Cerulean, Nuclear export signal (NES), T7 …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Lyn-GR-AKAR3

    Plasmid

    #173016

    Purpose

    Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    Lyn-GR-AKAR3
    

    Use

    Tags

    EGFP, N-terminal targeting sequence from Lyn kina…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3 14 mut B-Myb

    Plasmid

    #25967

    Depositor

    Rob Lewis

    Article

    Johnson et al J Biol Chem. 2002 Feb 8. 277(6):4088-97.

    Insert

    B-Myb (Mybl2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Changed threonines 267, 408, 443, 447 490, 497, 5…

    Promoter

    Available Since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1-Beta2-PAmcherry1

    Plasmid

    #114190

    Purpose

    studying clustering of Beta2 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Insert

    Adrenergic receptor beta 2 (ADRB2 Human)
    

    Use

    Tags

    3HA, FLAG, and PAmcherry1

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pY015 (pcDNA3.1-hLiCpf1)

    Plasmid

    #69987

    Purpose

    Expresses humanized LiCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLiCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

20. Y921F EphA2 pcDNA3

    Plasmid

    #102736

    Purpose

    Expression of EphA2 in mammalian cells

    Depositor

    Jin Chen

    Article

    Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

    Insert

    EphA2 (Epha2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y921F (TAC > TTC)

    Promoter

    CMV

    Available Since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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