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pcDNA3-pmEKAR4

Plasmid

#174440

Purpose

Plasma membrane-targeted EKAR4.

Depositor

Jin Zhang

Publication

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

pmEKAR4

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pTH-MYCN

Plasmid

#35416

Depositor

William A. Weiss

Publication

Weiss et al EMBO J. 1997 Jun 2;16(11):2985-95.

Insert

v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (MYCN Human)

Expression

Mammalian

Promoter

Tryosine Hydroxlyase (TH)

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA-GRK3

Plasmid

#32689

Depositor

Robert Lefkowitz

Publication

Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-1

Insert

GRK3 (GRK3 Bovine)

Expression

Mammalian

Promoter

CMV

Available since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pDONR207 SARS-CoV-2 NSP3_nostop

Plasmid

#149306

Purpose

Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pMKO.1-Puro-shPP2A-Abeta

Plasmid

#15250

Depositor

William Hahn

Publication

Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

Insert

PP2A regulatory subunit A, beta isoform (PPP2R1B Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
DR5rev::erRFP

Plasmid

#61011

Purpose

Auxin signalling reporter (DR5rev) driving RFP (ER-targeted)

Depositor

Alexis Maizel

Publication

Marin et al Plant Cell. 2010 Apr;22(4):1104-17. doi:

Insert

DR5rev::erRFP:35S_term

Expression

Plant

Promoter

DR5rev::erRFP:35Sterm

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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7TF

Plasmid

#24306

Depositor

Roel Nusse

Publication

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

7xTcf-FFluc

Use

Lentiviral

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pKH3-TRIM28-C65A/C68A

Plasmid

#92199

Purpose

Mammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)

Depositor

Huda Zoghbi

Publication

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

Tif1b (TRIM28 Synthetic, Human)

Tags

HA

Expression

Mammalian

Mutation

Changed Cysteines 65 and 68 to Alanines.

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRK5-mFzd7

Plasmid

#42259

Depositor

Chris Garcia, Jeremy Nathans

Publication

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 7 (Fzd7 Mouse)

Expression

Mammalian

Available since

March 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
TLCV2-APOBEC1-YTH

Plasmid

#178949

Purpose

Lentiviral vector for dox-inducible expression of APOBEC1-YTH in mammalian cells

Depositor

Kate Meyer

Publication

Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

Insert

APOBEC1-YTH-T2A-GFP

Use

Lentiviral and Synthetic Biology; Inducible

Tags

HA

Expression

Mammalian

Promoter

tight TRE

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLVX-IRES-Hygro_GCaMP6s

Plasmid

#164591

Purpose

Stable integration and expression of GCaMP6s in mammalian cells

Depositor

Joseph Hyser

Publication

Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

Insert

GCaMP6s

Use

Lentiviral

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pmGFP-P2A-K(AAA)12-P2A-RFP

Plasmid

#105687

Purpose

Double fluorescent stalling reporter K(AAA)12

Depositor

Ramanujan Hegde

Publication

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K(AAA)12
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDF-His-mTET1CD

Plasmid

#81053

Purpose

bacterial expression of the catalytic domain of murine TET1

Depositor

Primo Schaer

Publication

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Tags

6HIS

Expression

Bacterial

Mutation

catalytic domain amino acid 1367-2057

Promoter

T7

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLNCX1 HA Akt1

Plasmid

#15990

Depositor

Morris Birnbaum

Publication

Tuttle et al Nat Med. 2001 Oct . 7(10):1133-7.

Insert

Akt1 (Akt1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
YFP-Rac2

Plasmid

#11393

Depositor

Joel Swanson

Publication

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 2 (RAC2 Human)

Tags

YFP

Expression

Mammalian

Available since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJW1310

Plasmid

#61253

Purpose

Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion

Depositor

Jordan Ward

Publication

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

U6 promoter

Use

CRISPR; Cloning template

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRK5-mWnt5a

Plasmid

#42279

Depositor

Chris Garcia, Jeremy Nathans

Publication

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt5a (Wnt5a Mouse)

Expression

Mammalian

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pcDNA3.1 - BirA* (AA141-321) - HA - NIPP1

Plasmid

#86885

Purpose

C-terminal part of the split-BioID

Depositor

Mathieu Bollen

Publication

Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

Insert

BirA* (AA141-321)- HA- NIPP1]

Tags

HA tag

Expression

Mammalian

Mutation

BirA Amino Acids 141-321

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Blimp1pGL3 (Human Blimp1 promoter in pGL3-basic)

Plasmid

#40340

Depositor

Alexander Dent

Publication

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Insert

Blimp1 promoter (PRDM1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

Blimp1

Available since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV6M-Pak1 H83L H86L

Plasmid

#12211

Depositor

Jonathan Chernoff

Publication

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

PBD (p21-binding domain) mutant: H83L, H86L (see …

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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