We narrowed to 7,083 results for: mme

1. pRSET-TEV-12His

   Plasmid

   #137032

   Purpose

   empty vector; C-terminal TEV-protease-cleavable His12; T7 promoter; BseRI cloning sites (e.g., In-Fusion compatible)

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Type

   Empty backbone

   Use

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR_PGK_SNIPR_Notch1 del-NRR

   Plasmid

   #188353

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-del-NRR-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_Notch1-NRRdeletion-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. KTK_251

   Plasmid

   #180546

   Purpose

   Entry vector containing mScarlet

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Insert

   mScarlet
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGBT-R16

   Plasmid

   #31827

   Depositor

   Stephen Ekker

   Article

   Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

   Insert

   mini Tol2 ITR
   

   Use

   Cre/Lox; Gene trap

   Tags

   mRFP

   Expression

   Mutation

   mRFP lacks start codon

   Promoter

   beta Actin

   Available since

   Sept. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pExpreS2-1-C3-PA

   Plasmid

   #175446

   Purpose

   HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform

   Depositor

   Wyatt Yue

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Use

   Tags

   3C-Protein A and BiP Secretion Peptide

   Expression

   Insect

   Mutation

   Promoter

   Fused Actin-HSP70

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE2-EF1alpha-HA-TFAP4

   Plasmid

   #110158

   Purpose

   Lentiviral vector for expression of human TFAP4 in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

   Insert

   TFAP4 (transcription factor AP-4) (TFAP4 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSIN-Pdha1

   Plasmid

   #175936

   Purpose

   knockout mouse Pdha1

   Depositor

   Dan Littman

   Article

   Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

   Insert

   Pdha1 gRNA (Pdha1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. FFiBlast MSCV Puro

   Plasmid

   #68464

   Purpose

   Retroviral plasmid for establishment of blasticidin resistance Cre-reporter cell line

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Blasticidin Resistance
   

   Use

   Cre/Lox and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Inverted

   Promoter

   LTR

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. KA1396_pGL4-UBCmin-luc2/Klf4-E'

   Plasmid

   #124187

   Purpose

   Luciferase reporter driven by the Klf4 enhancer

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Insert

   Klf4 enhancer
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   UBCmin

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-HA-TFAP4(E135A/S139A)

    Plasmid

    #110157

    Purpose

    Expression of human TFAP4 (E135A/S139A) in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

    Insert

    TFAP4 (transcription factor AP-4) (TFAP4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E135A/S139A

    Promoter

    CMV

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. KTK_136

    Plasmid

    #180520

    Purpose

    Empty backbone for D1.1 cloning, contains sfGFP dropout

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJCC_058 FnCas12a D917A

    Plasmid

    #171667

    Purpose

    For bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    FnCas12a D917A
    

    Use

    Tags

    6X His and sortase A sorting motif

    Expression

    Bacterial

    Mutation

    changed aspartate 917 to alanine

    Promoter

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTK158_mCherry-4.1G-deltaCTD-hardened

    Plasmid

    #46360

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    4.1G (EPB41L2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Deleted amino acids 886-1005, and modified for si…

    Promoter

    CMV

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. KA2626_pGL4-UBCmin-luc2/Klf2-D'

    Plasmid

    #124199

    Purpose

    Luciferase reporter driven by the Klf2 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Klf2 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. KA1401_pGL4-UBCmin-luc2/Klf4-F'

    Plasmid

    #124188

    Purpose

    Luciferase reporter driven by the Klf4 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Klf4 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. KA1395_pGL4-UBCmin-luc2/Klf4-B'

    Plasmid

    #124186

    Purpose

    Luciferase reporter driven by the Klf4 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Klf4 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGK:HygroR-CMV:mKO2-C_UTR

    Plasmid

    #68481

    Purpose

    Lentivirus expressing mKO2 with UTR sensor cassette and hygromycin resistance

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    Hygromycin Resistance
    mKO2-C_UTR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    shRNA sensor cassette "B" added to 3&#x…

    Promoter

    CMV and pGK

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. NSSCP

    Plasmid

    #183190

    Purpose

    mRFP-PopZ expressed under J23116 promoter; split N terminal of TEV protease-SpmXΔC expressed under Tac promoter; SpmXΔC-split C terminal of TEV protease expressed under Tac promoter; DivIVA-sfGFP-TEV

    Depositor

    Hsiao-Chun Huang

    Article

    Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

    Inserts

    mRFP-PopZ
    DivIVA-GFP(tevR-50)
    SpmX-TEV(C)
    TEV(N)-SpmX
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAc5.1B-lambdaN-HA-DmDCP1_327-372-GST-V5His6_H

    Plasmid

    #146503

    Purpose

    Insect Expression of DmDCP1_327-372

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    DmDCP1_327-372 (Dcp1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLL7.0: Venus-oLID-Mito (From ActA)

    Plasmid

    #60414

    Purpose

    Mammalian Expression of Venus-oLID-Mito

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    Venus-oLID-Mito
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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