We narrowed to 5,828 results for: pcas

1. pJBL7345

   Plasmid

   #217372

   Purpose

   sfGFP reporter for chimeric riboswitch with Cbe pfl ZTP riboswitch aptamer and WT Pca rhtB ZTP riboswitch expression platform

   Depositor

   Julius Lucks

   Article

   Bushhouse et al bioRxiv 2024.03.29.587317

   Insert

   Chimeric ZTP riboswitch with Cbe pfl aptamer and WT Pca rhtB expression platform sfGFP reporter
   

   Available Since

   Nov. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEcgRNA

   Plasmid

   #166581

   Purpose

   CRISPR-Cas9-assisted genome editing in Escherichia coli

   Depositor

   Sheng Yang

   Article

   Li et al Acta Biochim Biophys Sin (Shanghai). 202

   Insert

   ccdB
   

   Expression

   Bacterial

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. p131C-B10

   Plasmid

   #241908

   Purpose

   Optimised PCA biosensor produced sfGFP in response to hydroxyl-substituted benzoic acids, including protocatechuic acid (PCA)

   Depositor

   Neil Dixon

   Article

   Berepiki et al ACS Synth Biol. 2020 Mar 20;9(3):576-589

   Insert

   pcaV, sfGFP
   

   Use

   Synthetic Biology

   Mutation

   none

   Available Since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEcCas

   Plasmid

   #73227

   Purpose

   Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA

   Depositor

   Sheng Yang

   Article

   Li et al Acta Biochim Biophys Sin (Shanghai). 202

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR; Red recombinase

   Expression

   Bacterial

   Promoter

   native promoter

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJBL6951

   Plasmid

   #217373

   Purpose

   sfGFP reporter for Bsu pbuE* 2AP riboswitch

   Depositor

   Julius Lucks

   Article

   Bushhouse et al bioRxiv 2024.03.29.587317

   Insert

   Pca rhtB riboswitch sfGFP reporter
   

   Available Since

   Nov. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221_FLVCR1

   Plasmid

   #131892

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   FLVCR1 (FLVCR1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pDONR221-FLVCR1_STOP

   Plasmid

   #161058

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   FLVCR1 (FLVCR1 Human)
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLCNICK

   Plasmid

   #84653

   Purpose

   Genome editing for Lactobacillus casei Lc2W

   Depositor

   Sheng Yang

   Article

   Song et al Appl Environ Microbiol. 2017 Sep 1. pii:

   Inserts

   Cas9 nickase
   sgRNA
   homology arms of LC2W_2179
   

   Use

   CRISPR

   Mutation

   D10A

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. CRISPRmTmG2

   Plasmid

   #69992

   Purpose

   The CRISPR construct targets near the LoxP sites in Rosa-pCA-loxP-mTdtomato-loxP-mEGFP mice.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   gRNA that targets near LoxP sites
   

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. p44PPV-eGFP

    Plasmid

    #241907

    Purpose

    Part 2 of a two-plasmid PCA biosensor composed of the PcaV repressible PPV promoter upstream from a reporter gene (eGFP) on a second plasmid

    Depositor

    Neil Dixon

    Article

    F M Machado et al J Biol Eng. 2019 Nov 27;13:91. doi: 10.1

    Insert

    His6-eGFP
    

    Use

    Synthetic Biology

    Mutation

    none

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHSP02

    Plasmid

    #117259

    Purpose

    Genome editing for Lactobacillus plantarum WCSF1

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    Cas9, promotor P11-guide-RNA, homologous arms of Lp_0537
    

    Use

    Other

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. p131CB10-tphKAB

    Plasmid

    #241911

    Purpose

    TPA catabolic operon under the control of a PCA inducible biosensor

    Depositor

    Neil Dixon

    Article

    Chacon et al Trends Biotechnol. 2025 Mar;43(3):647-67

    Insert

    tphK, tphA2, tphA3, tphB, tphA1
    

    Use

    Synthetic Biology

    Mutation

    none

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHSB04X

    Plasmid

    #117258

    Purpose

    Genome editing for Lactobacillus brevis ATCC367

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    Cas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
    

    Use

    Other

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pISFba1

    Plasmid

    #226820

    Purpose

    Expressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISFba1 guide RNA targeting pMB1 ori

    Depositor

    Sheng Yang

    Article

    Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

    Insert

    ISFba1
    

    Expression

    Bacterial

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pISEre1

    Plasmid

    #226819

    Purpose

    Expressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISEre1 guide RNA targeting pMB1 ori

    Depositor

    Sheng Yang

    Article

    Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

    Insert

    ISEre1
    

    Expression

    Bacterial

    Available Since

    Oct. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCY5h

    Plasmid

    #160295

    Purpose

    Yeast CRISPR plasmid targeting the hphMX cassette

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCY5n

    Plasmid

    #160296

    Purpose

    Yeast CRISPR plasmid targeting the natMX cassette

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCY5k

    Plasmid

    #160294

    Purpose

    Yeast CRISPR plasmid targeting the kanMX cassette

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKB12 (pDEST-DHFR F[3] C-term, HygR)

    Plasmid

    #210486

    Purpose

    pDEST vector to tag gene of interest with DHFR F[3] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCY5.1kh

    Plasmid

    #160297

    Purpose

    Yeast CRISPR plasmid targeting the kanMX and hphMX cassettes

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
    URA3MX selection cassette
    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Available Since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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