We narrowed to 10,632 results for: t7

1. pPSU150

   Plasmid

   #173836

   Purpose

   Expresses 150 kD Penn State ladder protein

   Depositor

   Song Tan

   Article

   Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

   Insert

   STRHSTPABMBPpepN
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPSU250

   Plasmid

   #173837

   Purpose

   Expresses 250 kD Penn State ladder protein

   Depositor

   Song Tan

   Article

   Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

   Insert

   STRHSTPABPACIL1bQRSSTRHSTPABMBPpepN
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET23a-mUbc9

   Plasmid

   #53137

   Purpose

   E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

   Depositor

   Frauke Melchior

   Article

   Pichler et al Cell. 2002 Jan 11;108(1):109-20.

   Insert

   Ubc9 (Ube2i Mouse)
   

   Tags

   untagged

   Expression

   Bacterial

   Mutation

   protein sequence is identical to human

   Promoter

   T7

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28 MBP_PrA/G-H6

   Plasmid

   #176077

   Purpose

   Express His6 tagged MBP_PrA/G in Escherichia coli

   Depositor

   Tom Rapoport

   Article

   Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   MBP PrA/G-H6
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET_RTX

   Plasmid

   #102787

   Purpose

   Expresses the Reverse Transcriptase gene

   Depositor

   Andrew Ellington

   Article

   Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

   Insert

   reverse transcription xenopolymerase
   

   Expression

   Bacterial

   Promoter

   T7 promoter + lac operator

   Available Since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Sfp pet29b C-terminal His Tag

   Plasmid

   #75015

   Purpose

   Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

   Depositor

   Michael Burkart

   Article

   Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

   Insert

   Sfp
   

   Tags

   His Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET N6his GFP1-9

   Plasmid

   #182240

   Purpose

   Tripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.

   Depositor

   Stéphanie Cabantous

   Article

   Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

   Insert

   GFP1-9
   

   Tags

   6HIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21-Streptavidin-Glutamate_Tag

   Plasmid

   #46367

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

   Insert

   Streptavidin-Glutamate_Tag
   

   Tags

   6 glutamate tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pET28a(+)/FbGH30

   Plasmid

   #86463

   Purpose

   Expression of the Beta-1,6-Exo-Laminarinase FbGH30 in E.coli

   Depositor

   Jan-Hendrik Hehemann

   Article

   Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

   Insert

   FbGH30
   

   Tags

   His-Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSETA_crGE

    Plasmid

    #172927

    Purpose

    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.

    Depositor

    Arnold Boersma

    Article

    Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

    Insert

    CrowdingSensorGE
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28c-F30-Broccoli

    Plasmid

    #66788

    Purpose

    Expresses Broccoli in the F30 scaffold in bacterial cells

    Depositor

    Samie Jaffrey

    Article

    Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

    Insert

    Broccoli in the F30 scaffold
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

    Plasmid

    #225755

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. 6xHis-MBP-huMb2Cas12a

    Plasmid

    #174676

    Purpose

    Expresses 6xHis-MBP tagged Mb2Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huMb2Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDJSVT87

    Plasmid

    #109302

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Tags

    OmpA 1-27, 6His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pND004

    Plasmid

    #200950

    Purpose

    MBP-tagged E. coli protein expression destination vector for Golden Gate cloning

    Depositor

    Timothy Whitehead

    Article

    Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

    Type

    Empty backbone

    Tags

    MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET11a-Z-NspGFP

    Plasmid

    #40729

    Depositor

    Brian McNaughton

    Article

    Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub

    Insert

    N super positive GFP - Leucine Zipper
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSFDuet1_CylLL_CylM

    Plasmid

    #208759

    Purpose

    Expresses modified cytolysin L (mCylLL) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

    Inserts

    CylLL
    CylLM
    

    Tags

    Hisx6 Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

    Plasmid

    #225756

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. 6xHis-MBP-huTsCas12a

    Plasmid

    #174687

    Purpose

    Expresses 6xHis-MBP tagged TsCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huTsCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-21a(+)-IPP1-His

    Plasmid

    #118978

    Purpose

    Bacterial expression plasmid for Inorganic pyrophosphatase

    Depositor

    Sebastian Maerkl

    Article

    Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

    Insert

    inorganic diphosphatase IPP1 (IPP1 Budding Yeast)
    

    Tags

    6x-His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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