We narrowed to 14,145 results for: TIM

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PIP5K1A gRNA (BRDN0001147420)

Plasmid

#77964

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIP5K1A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIP5K1A (PIP5K1A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIP5K1B gRNA (BRDN0001147624)

Plasmid

#77083

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIP5K1B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIP5K1B (PIP5K1B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PTK6 gRNA (BRDN0001149364)

Plasmid

#76490

Purpose

3rd generation lentiviral gRNA plasmid targeting human PTK6

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PTK6 (PTK6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ADCK4 gRNA (BRDN0001145019)

Plasmid

#76560

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADCK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADCK4

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCNL-C1_fibrillarin

Plasmid

#65709

Purpose

Expresses CNL-fibrillarin in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

fibrillarin (FBL Human)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
pET-dHeFSpCas9-VP64-6xHis

Plasmid

#92119

Purpose

Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive HeFSpCas9-NLS-3xFLAG-VP64

Use

CRISPR

Tags

3xFLAG, 6xHis, NLS, and VP64

Expression

Bacterial

Mutation

D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

Promoter

T7

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001145269)

Plasmid

#80249

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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ROCK2 gRNA (BRDN0001146481)

Plasmid

#76350

Purpose

3rd generation lentiviral gRNA plasmid targeting human ROCK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ROCK2 (ROCK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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sTR056

Plasmid

#177369

Purpose

Toehold switch, with an unpaired toehold region at the 5′-end that interacts with the trigger RNA (sTR060, Addgene plasmid # 177370) to unfold the switch.

Depositor

Jerome Bonnet, Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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sTR060

Plasmid

#177370

Purpose

Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

Depositor

Jerome Bonnet, Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Insert

Trigger RNA

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001148372)

Plasmid

#80258

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pET-45b-NFYC-IDR-mEGFP

Plasmid

#185015

Purpose

Bacterial Expression of 6xHis NFYC IDR Sirius-GFP Fusion Protein

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

NFYC IDR (Nfyc Mouse)

Expression

Bacterial

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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NUAK1 gRNA (BRDN0001145557)

Plasmid

#75575

Purpose

3rd generation lentiviral gRNA plasmid targeting human NUAK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NUAK1 (NUAK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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T7T3-PacI-elfn1_459to1199

Plasmid

#87671

Purpose

In situ probe for mouse Elfn1

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Elfn1 (Elfn1 Mouse)

Expression

Bacterial

Mutation

partial sequence, (459 to 1199bp off NM_175522.3)

Promoter

T7/T3

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426)

Plasmid

#133788

Purpose

CAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized St3Cas9

Tags

NLS(SV40)-3xFLAG

Expression

Mammalian

Mutation

n/a

Promoter

CAG

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001145885)

Plasmid

#80196

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pMCSF-R(m40)-luc

Plasmid

#12425

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1994 Jan . 14(1):373-81.

Insert

M-CSF receptor promoter fragment (CSF1R Human)

Use

Luciferase

Mutation

PU.1 binding site mutation (created a KpnI site i…

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)

Plasmid

#174642

Purpose

Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)

Tags

SSPB(micro)-Venus

Expression

Mammalian

Mutation

SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

Promoter

Chicken beta-actin

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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PIP5K1A gRNA (BRDN0001487080)

Plasmid

#77962

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIP5K1A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIP5K1A (PIP5K1A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIP5K1B gRNA (BRDN0001146307)

Plasmid

#77085

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIP5K1B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIP5K1B (PIP5K1B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PIP5K1B gRNA (BRDN0001148929)

Plasmid

#77086

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIP5K1B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIP5K1B (PIP5K1B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRPS2 gRNA (BRDN0001147559)

Plasmid

#78005

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRPS2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRPS2 (PRPS2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PRPS2 gRNA (BRDN0001487102)

Plasmid

#78006

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRPS2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRPS2 (PRPS2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pONL-C1_Rab11a

Plasmid

#65707

Purpose

Expresses ONL-Rab11a in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Rab11a (Rab11a Mouse)

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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ADCK3 gRNA (BRDN0001147832)

Plasmid

#77321

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADCK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADCK3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
ADCK3 gRNA (BRDN0001146491)

Plasmid

#77323

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADCK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADCK3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2-7xTcf-NLS-ONL-CP

Plasmid

#65716

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, ONL, hCL1-PEST

Use

Luciferase

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-NHASt-codoptUL50

Plasmid

#135101

Purpose

Codon-optimized HSV-1 UL50 with N-terminal HAStrep tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL50 codon optimized

Tags

HA-Strep-Strep

Expression

Mammalian

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-A0LWB3

Plasmid

#103070

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

A0LWB3(Cas9 coding gene from Acidothermus cellulolyticus (strain ATCC 43068 / 11B))

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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ADRBK2 gRNA (BRDN0001487158)

Plasmid

#77943

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADRBK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADRBK2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge