We narrowed to 11,432 results for: 112

1. mIFP-CytERM-N-17

   Plasmid

   #56217

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   CytERM
   

   Use

   Tags

   mIFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pCMV-HA-mIFITM3-K83,88,104A

   Plasmid

   #58405

   Purpose

   Expresses murine IFITM3-K83,88,104A with an N-terminal HA tag in mammalian cells

   Depositor

   Jacob Yount, Howard Hang

   Article

   Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

   Insert

   IFITM3 (Ifitm3 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Lysines 83, 88, and 104 to Alanine

   Promoter

   CMV IE

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pCMV-HA-mIFITM3-K83A

   Plasmid

   #58401

   Purpose

   Expresses murine IFITM3-K83A with an N-terminal HA tag in mammalian cells

   Depositor

   Jacob Yount, Howard Hang

   Article

   Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

   Insert

   IFITM3 (Ifitm3 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Lysine 83 to Alanine

   Promoter

   CMV IE

   Available since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCMV-HA-mIFITM3-K24A

   Plasmid

   #58400

   Purpose

   Expresses murine IFITM3-K24A with an N-terminal HA tag in mammalian cells

   Depositor

   Jacob Yount, Howard Hang

   Article

   Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

   Insert

   IFITM3 (Ifitm3 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Lysine 24 to Alanine

   Promoter

   CMV IE

   Available since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

5. GST-TEVcs-GFP-ATG101

   Plasmid

   #171415

   Purpose

   For the purifcation of GFP-ULK1 complex

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   ATG101 (ATG101 Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GST-GFP-ATG14

   Plasmid

   #171426

   Purpose

   For the purifcation of GFP- PI3KC3 complex

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   ATG14 (ATG14 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pACYC-OspCas12c-noncoding

   Plasmid

   #120878

   Purpose

   Expresses concatenated noncoding sequences surrounding the CRISPR-OspCas12c system in the native genomic loci; expresses the tracrRNA required for activity.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Noncoding sequences for CRISPR-OspCas12
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. UKNP4.3.1

   Plasmid

   #98269

   Purpose

   Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

   Depositor

   Jonathan Ball

   Article

   Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

   Insert

   HCV E1E2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. UKNP3.2.2

   Plasmid

   #98247

   Purpose

   Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

   Depositor

   Jonathan Ball

   Article

   Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

   Insert

   HCV E1E2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-myc-mCE(K294A)

    Plasmid

    #82461

    Purpose

    Expression of myc epitope and catalytic inactive form of murine mRNA Capping Enzyme (K294A) in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    mCE (K294A) (Rngtt Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Lysine 294 changed to Alanine

    Promoter

    CMV

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. UKNP1.6.1

    Plasmid

    #97370

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. UKNP1.5.3

    Plasmid

    #97369

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. UKNP1.18.2

    Plasmid

    #98191

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. UKNP1.11.4

    Plasmid

    #97380

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. UKNP1.11.3

    Plasmid

    #97379

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. UKNP1.11.2

    Plasmid

    #97378

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. UKNP1.11.1

    Plasmid

    #97377

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSUM-GroEL-gfp

    Plasmid

    #109286

    Purpose

    Expresses gfp in mycobacteria

    Depositor

    Nicolai van Oers

    Article

    Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

    Insert

    GroEL-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GFP modified for expression in prokaryotes; inclu…

    Promoter

    GroEL

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMU103

    Plasmid

    #73934

    Purpose

    This is hpRNA construct for gene silencing in soybean seeds. Soybean seed specific promoter, Glycinin gene promoter, is used.

    Depositor

    Zhanyuan Zhang

    Article

    Flores et al Transgenic Res. 2008 Oct;17(5):839-50. d

    Insert

    bar and GUS cassettes
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. UKNP1.1.1

    Plasmid

    #97355

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. UKNP2.1.2

    Plasmid

    #98208

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. UKNP2.1.1

    Plasmid

    #98207

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. UKNP1.22.1

    Plasmid

    #98205

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. UKNP1.21.6

    Plasmid

    #98204

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. UKNP1.21.4

    Plasmid

    #98202

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSum-LacZ-gfp

    Plasmid

    #109285

    Purpose

    Expresses gfp in mycobacteria

    Depositor

    Nicolai van Oers

    Article

    Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GFP modified for expression in prokaryotes; inclu…

    Promoter

    LacZalpha

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MiniCoopR mitfa:SPRED1

    Plasmid

    #118851

    Purpose

    Expresses human SPRED1 and zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    SPRED1 (SPRED1 Human, Zebrafish)
    

    Use

    Zebrafish expression, tol7

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET27b-ecDHFR-N23PP/S148A

    Plasmid

    #110829

    Purpose

    Bacterial expression of E.coli DHFR N23PP/S148A mutant

    Depositor

    Stephen Benkovic

    Article

    Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N23 mutated to P23; a proline residue inserted be…

    Promoter

    T7

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. JM3 [JBEI-6891]

    Plasmid

    #89788

    Purpose

    Broad host range vector pBBR1-MCS2 containing 'tesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli) under control of BAD promoter

    Depositor

    Harry Beller

    Article

    Muller et al Appl Environ Microbiol. 2013 Jul;79(14):

    Insert

    tesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTrc99S-ssDsbA-PD-4xDQNAT

    Plasmid

    #128399

    Purpose

    Periplasmic expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    H. influenzae protein D
    

    Use

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pTrc99S-ssDsbA-PorA-4xDQNAT

    Plasmid

    #128400

    Purpose

    Periplasmic expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    N. meningitidis PorA
    

    Use

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115214

    Purpose

    Expresses PauCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PauCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pKD011 CMV 3xFLAG-NLS-ZF2-ABI1 in TUPV2

    Plasmid

    #161548

    Purpose

    Constitutive expression of 3xFLAG-NLS-ZF2-ABI1 under the CMV promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-ZF2-ABI1
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBbdCas9S(RBSmut)_Psyn-sgRNArodA

    Plasmid

    #149656

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJM582 EF1α 3xFLAG-NLS-VP64-ZF2 in TUPV2

    Plasmid

    #161543

    Purpose

    Constitutive expression of 3xFLAG-NLS-VP64-ZF2 under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-VP64-ZF2
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJM465 EF1α 3xFLAG-NLS-VP64-ZF1 in TUPV2

    Plasmid

    #161533

    Purpose

    Constitutive expression of 3xFLAG-NLS-VP64-ZF1 under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-VP64-ZF1
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pQTEV-TCTEL1

    Plasmid

    #31337

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    t-complex-associated-testis-expressed 1-like 1 (DYNLT1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense

    Plasmid

    #46839

    Purpose

    Express cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translated

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pENTR_Hu_Plk5(WT)

    Plasmid

    #136329

    Purpose

    Human Plk5 WT in Gateway pENTR

    Depositor

    Marcos Malumbres, Guillermo de Cárcer

    Article

    de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

    Insert

    PLK5 (PLK5 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. OphA-delta C6

    Plasmid

    #127679

    Purpose

    Bacterial expression of His-tagged OphA-delta C6

    Depositor

    James Naismith

    Article

    Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

    Insert

    OphA-delta C6
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    delta C6

    Promoter

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA3.1(+)-TTF-1-Q50K-Y54M

    Plasmid

    #75264

    Purpose

    Expresses a double point mutant of human TTF-1

    Depositor

    David Mu

    Article

    Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

    Insert

    TTF-1 (NKX2-1 Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Q50K and Y54M in the homeodomain

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

42. pSPPac-hp

    Plasmid

    #48123

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Pac under control of the xylose inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

    Insert

    signal peptide of Pac
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAL950

    Plasmid

    #119748

    Purpose

    precursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    precusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Internal NdeI site removed and mutations Alanine …

    Promoter

    T7

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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44. pSPNprM-hp

    Plasmid

    #48120

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein NprM under control of the xylose inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

    Insert

    signal peptide of NprM
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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45. UKNP1.19.1

    Plasmid

    #98192

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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46. pSG5HA-MAGE-KA240,245

    Plasmid

    #89140

    Purpose

    mammalian expression of N-terminal HA-tagged full-length human MAGE-A11 amino acid residues 2-429 with KA240,KA245 ubiquitination sites mutated

    Depositor

    Elizabeth Wilson

    Article

    Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

    Insert

    MAGE-A11 (MAGEA11 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K240A, K245A

    Promoter

    SV40

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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47. ZJOM8

    Plasmid

    #133671

    Purpose

    Integration of SNF7-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on endosomes and vacuolar surface.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-SNF7-GFP (SNF7 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.14 Sense

    Plasmid

    #46838

    Purpose

    Express cGFP transcript ending in the minimal functional MALAT1 triple helix

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCEP4-SHIV327C-HuA10-gp160

    Plasmid

    #123270

    Purpose

    Mammalian expression plasmid for Env from the SHIV327C-Hu A10 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    SHIV327C-Hu A10 Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. pOGG113

    Plasmid

    #133124

    Purpose

    pL0M-PU-NoP, promotorless region used as negative control

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    NoP promotorless region from plasmid pIJJ11268
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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