We narrowed to 22,374 results for: ESC;

1. iOn-CAG∞RFP

   Plasmid

   #154014

   Purpose

   Vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

   Depositor

   Jean Livet

   Article

   Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

   Insert

   mRFP1
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDuEx2-NIR-GECO1

   Plasmid

   #113680

   Purpose

   Bacterial and mammalian expression plasmid for near-infrared fluorescent Ca2+ indicator NIR-GECO1

   Depositor

   Robert Campbell

   Article

   Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

   Insert

   NIR-GECO1
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. H2B-mGreenLantern

   Plasmid

   #164464

   Purpose

   Localization of mGreenLantern fluorescent protein to histone 2B

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Tags

   Human Histone 2B (D26G and V119I mutations)

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CMV

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHR-CMV_TetR-HA-NLS-P2A-BSD-Myc

   Plasmid

   #113899

   Purpose

   Mammalian constitutive expression of HA-tagged Tetracyclin repressor protein and Myc-tagged blasticidin deaminase

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Insert

   TetR-HA-NLS-P2A-BSD-Myc
   

   Use

   Lentiviral

   Tags

   HA-Myc

   Promoter

   CMV-MIE

   Available Since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. single polypeptide FPX biosensor for calcium ion

   Plasmid

   #60887

   Purpose

   FPX biosensor

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   single polypeptide FPX biosensor for calcium ion
   

   Tags

   HindIII site

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNCST-AausFP1

   Plasmid

   #129499

   Purpose

   Expresses AausFP1 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AausFP1
   

   Expression

   Bacterial

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP_sfCherry2(11)_Clathrin light chain

   Plasmid

   #83032

   Purpose

   Expresses sfCherry2(11) tagged clathrin light chain in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry2(11)_Clathrin light chain (CLTA Synthetic, Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Cx43-msfGFP

   Plasmid

   #69007

   Purpose

   Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.

   Depositor

   David Spray

   Article

   Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

   Insert

   Gja1 (Gja1 Rat)
   

   Tags

   monomerized superfolder GFP

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-hSyn-GRAB_NE1h

   Plasmid

   #123309

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neurons

   Depositor

   Yulong Li

   Article

   Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

   Has Service

   AAV Retrograde and AAV9

   Insert

   GPCR activation based NE sensor GRAB_NE1h
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LifeAct-mGreenLantern

    Plasmid

    #164459

    Purpose

    Localization of mGreenLantern fluorescent protein to actin

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Tags

    LifeAct

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Clathrin-mGreenLantern

    Plasmid

    #164462

    Purpose

    Localization of mGreenLantern fluorescent protein to clathrin

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Tags

    Clathrin

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LiOn-CAG∞IRFP

    Plasmid

    #154017

    Purpose

    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein IRFP670 from a CAG promoter upon action of the piggyBac transposase

    Depositor

    Jean Livet

    Article

    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

    Insert

    IRFP670
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Orai1-jGCaMP8f

    Plasmid

    #231630

    Purpose

    Green fluorescent reporter of Orai1-associated calcium influx

    Depositor

    Michael Cahalan

    Article

    Dynes et al Proc Natl Acad Sci U S A. 2023 Sep 26;12

    Insert

    Orai1-jGCaMP8f (ORAI1 Synthetic, Human)
    

    Tags

    jGCaMP8f

    Expression

    Mammalian

    Promoter

    CMV Immediate Early

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBAD-K-GECO1

    Plasmid

    #105864

    Purpose

    Bacterial expression plasmid for red fluorescent Ca2+ indicator K-GECO1

    Depositor

    Robert Campbell

    Article

    Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

    Insert

    K-GECO1
    

    Tags

    6-His Tag

    Expression

    Bacterial

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER

    Plasmid

    #113898

    Purpose

    Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligase

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Avi-His6

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-BLRR

    Plasmid

    #158958

    Purpose

    Bioluminescent repair reporter (BLRR) for DNA double strand break (DSB) repairs: homology-directed repair (HDR) and non-homologous end joining (NHEJ).

    Depositor

    Charles P. Lai

    Article

    Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

    Insert

    BLRR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMito-miRFP703

    Plasmid

    #80000

    Purpose

    Mitochondria labeling with near-infrared fluorescent protein miRFP703

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    cytochrome C oxidase subunit VIII (COX8A Human)
    

    Tags

    miRFP703

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Cx26-msfGFP

    Plasmid

    #69016

    Purpose

    Expresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx26 (Gjb2 Rat)
    

    Tags

    V206K monomerized super folder GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGP-AAV-syn-flex-iGABASnFR2(no bind)-WPRE

    Plasmid

    #218879

    Purpose

    AAV-mediated expression of improved GABA sensor control (no change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Has Service

    AAV1 and AAV5

    Insert

    iGABASnFR2(no bind)
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    S99A F102G R168P

    Promoter

    Synapsin

    Available Since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn-GRAB_NEmut

    Plasmid

    #123310

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neurons

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Has Service

    AAV Retrograde and AAV9

    Insert

    GPCR activation based NE control sensor GRAB_NEmut
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge