We narrowed to 5,563 results for: org

1. pWZL-hygro-FAKS732A

   Plasmid

   #216541

   Purpose

   This retroviral plasmid expresses a mutated cDNA (dominant negative: S732A) of human PTK2

   Depositor

   Georgia Konstantinidou

   Article

   Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

   Insert

   PTK2 (S732A) (PTK2 Human)
   

   Use

   Retroviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   FAK dominant negative mutation S732A

   Promoter

   Available since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pWZL-hygro-FAKS910A

   Plasmid

   #216542

   Purpose

   This retroviral plasmid expresses a mutated cDNA (dominant negative: S910A) of human PTK2

   Depositor

   Georgia Konstantinidou

   Article

   Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

   Insert

   PTK2 (S910A) (PTK2 Human)
   

   Use

   Retroviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   FAK dominant negative mutation S910A

   Promoter

   Available since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1_V5-TWIST1_P139S

   Plasmid

   #215040

   Purpose

   Mammalian expression of human TWIST1 (with P139S mutation)

   Depositor

   Joanna Wysocka

   Article

   Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

   Insert

   twist family bHLH transcription factor 1 (TWIST1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   P139S

   Promoter

   CMV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1_V5-TWIST1_L138Y

   Plasmid

   #215041

   Purpose

   Mammalian expression of human TWIST1 (with L138Y mutation)

   Depositor

   Joanna Wysocka

   Article

   Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

   Insert

   twist family bHLH transcription factor 1 (TWIST1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   L138Y

   Promoter

   CMV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1_V5-TWIST1_L138F

   Plasmid

   #215042

   Purpose

   Mammalian expression of human TWIST1 (with L138F mutation)

   Depositor

   Joanna Wysocka

   Article

   Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

   Insert

   twist family bHLH transcription factor 1 (TWIST1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   L138F

   Promoter

   CMV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV_MSX1-FKBP-mNeonGreen-V5

   Plasmid

   #215025

   Purpose

   AAV vector for knocking in a C-terminal tag (FKBP12F36V, mNeonGreen, and V5) for human MSX1

   Depositor

   Joanna Wysocka

   Article

   Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

   Insert

   msh homeobox 1 (MSX1 Human)
   

   Use

   AAV

   Tags

   FKBP-mNeonGreen-V5

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCRISPR-cBEST-v2-SF14P

   Plasmid

   #209448

   Purpose

   improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoter

   Depositor

   Tilmann Weber

   Article

   Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

   Insert

   Codon optimized APOBEC1-nCas9-UGI fusion protein
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PtipA; sgRNA expression from SF14P promoter

   Available since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRISPR-cBEST-v2-kasOP*

   Plasmid

   #209447

   Purpose

   improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoter

   Depositor

   Tilmann Weber

   Article

   Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

   Insert

   Codon optimized APOBEC1-nCas9-UGI fusion protein
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PtipA; sgRNA expression from kasOP* promoter

   Available since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-DONSON-DEWS

   Plasmid

   #208799

   Purpose

   Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A, S437A)

   Depositor

   Aga Gambus

   Article

   Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

   Insert

   DONSON (donson.L Frog)
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial

   Mutation

   D374A; E377A; W381A; S437A

   Promoter

   T7

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-DONSON-3A

    Plasmid

    #208800

    Purpose

    Xenopus laevis His6-Myc-DONSON (V473A, R476A, Y481A)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    V473A; R476A; Y481A

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-DONSON-Δ24

    Plasmid

    #208801

    Purpose

    Xenopus laevis His6-Myc-DONSON (1-24aa removed)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Δ24, deletion of 1-24aa

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-DONSON-DEW

    Plasmid

    #208802

    Purpose

    Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    D374A; E377A; W381A

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FKBP12-CB2mutx2-mTagBFP2-HAx2

    Plasmid

    #207147

    Purpose

    Tandem mutant calcium-buffer with rapalog dimerizer

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    Calbindin-2 (CALB2 Human)
    

    Use

    Tags

    FKBP12, HA, and mTagBFP2

    Expression

    Mammalian

    Mutation

    E40Q, E87Q, E131Q, E175Q, E219Q

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST40-CES2C-ΔC

    Plasmid

    #200555

    Purpose

    Mammalian expression of secreted mouse CES2C

    Depositor

    Jonathan Long

    Article

    Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

    Insert

    Mouse Carboxylesterase 2C delta C-terminal (Ces2c Mouse)
    

    Use

    Tags

    6xHis, Flag, and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRK5 PSK (745-1235)

    Plasmid

    #197863

    Purpose

    Expression of C-terminal PSK1 / TAOK2 (aa745-1235) in mammalian cells (microtubule-binding domain)

    Depositor

    Jonathan Morris

    Article

    Mitsopoulos et al J Biol Chem. 2003 May 16;278(20):18085-9

    Insert

    PSK1 (745-1235) (TAOK2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    The N terminus of PSK1/TAOK2 has been deleted

    Promoter

    SV40

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pShuttle.CC9

    Plasmid

    #192931

    Purpose

    Gateway compatible vector with BsaI cloning site and Cre-2A-Cas9 expression

    Depositor

    Thorsten Stiewe

    Article

    Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

    Type

    Empty backbone

    Use

    Gateway-compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KTK_139

    Plasmid

    #180565

    Purpose

    Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KTK_113

    Plasmid

    #180540

    Purpose

    Entry vector containing Signal peptide region of CsgA including SecB-N22 sequenced

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    Signal peptide region of CsgA including SecB-N22 sequenced
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pvLEA22mer_shuffle_3_mCh-2xFKBP (pBS1119)

    Plasmid

    #185314

    Purpose

    For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    pvLEA22mer_shuffle_3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pST_Q19228_CAEEL_93-127 (pBS0735)

    Plasmid

    #185231

    Purpose

    For the mammalian expression of the C. elegans protein Q19228_CAEEL_93-127. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    Q19228_CAEEL_93-127
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge