-
-
pHR_UCOE-SFFV-dCas9-XTEN80-KRAB(Kox1)-IRES-mCherry
Plasmid#188770PurposedCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)DepositorInsertdCas9-XTEN80-KRAB(Kox1)
UseLentiviralTagsHA-2xNLS-XTEN80(linker)-KRAB(Kox1)ExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_intergenic-intergenic_1
Plasmid#155077PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectivelyDepositorInsert(hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_intergenic-intergenic_2
Plasmid#155078PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectivelyDepositorInsert(hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S87A
Plasmid#36058DepositorInsertalpha-synuclein S87A (SNCA Human)
UseTagsExpressionBacterialMutationS87APromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
-
pCS3-MT-BX-6MYC p21
Plasmid#78780PurposeTo overexpress p21 in Mammalian CellsDepositorInsertp21 (CDKN1A Human)
UseTags6xMYCExpressionMammalianMutationPromoterCMV IE94Available sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_Ptbp_ex8_3
Plasmid#155083PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)DepositorInsert(hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
LIN7A_PDZ_1
Plasmid#103918PurposeProtein expression and purification of LIN7 PDZ domainDepositorInsertLIN7A (LIN7A Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Flag-Avi-ires-CHD5
Plasmid#68878PurposeTransient mammalian expression of CHD5 for BirA taggingDepositorInsertCHD5 (CHD5 Human)
UseTagsFLAG/AVIExpressionMammalianMutationPromoterCAGAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMinDis.X513-iNicSnFR3b-(V7)-PM
Plasmid#125124PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotineDepositorInsertiNicSnFR3b-(V7)-PM
UseTagsIg-K-Chain and membrane anchoring protein-PDGFRExpressionMammalianMutationPromoterT7Available sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-mVenus-FLAG-LANS1-Set2
Plasmid#122003PurposemVenus-FLAG-LANS-Set2: mVenus- and FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeastDepositorInsertmVenus-FLAG-LANS1-Set2 (SET2 Budding Yeast)
UseSynthetic BiologyTagsmVenus-FLAGExpressionYeastMutationSet2 point mutations K538G, K539S, R549G, K550S, …PromoterADH1Available sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
DLG1_PDZ_3
Plasmid#103917PurposeProtein expression and purification of DLG1 PDZ3 domainDepositorInsertDLG1 (DLG1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV TIP60 212-364
Plasmid#78795PurposeTo overexpress TIP60 212-364 in Mammalian CellsDepositorInsertTIP60 (KAT5 Human)
UseTags3xFLAGExpressionMammalianMutation212-364, G214APromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
DLG3_PDZ_3
Plasmid#103948PurposeProtein expression and purification of NE-DLG PDZ3 domainDepositorInsertDLG3 (DLG3 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_TK1_HPRT1_Lb_4
Plasmid#155062PurposeLentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a (CHyMErA system)DepositorInsert(hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-CHD5(Chromos)-FRAG
Plasmid#68875PurposeIPTG inducible bacterial expression of CHD5 fragmentDepositorInsertCHD5 (CHD5 Human)
UseTagsGSTExpressionBacterialMutationonly the two chromodomains of CHD5PromotertacAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
DLG1_PDZ_2
Plasmid#103916PurposeProtein expression and purification of DLG1 PDZ2 domainDepositorInsertDLG1 (DLG1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL
Plasmid#172195PurposeAAV sequence-specific control vector for TALE-Tet1FL-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.DepositorInsertHA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL
UseAAV and Mouse Targeting; TaleTagsHA, NLS, and T2A-EGFPExpressionMammalianMutationtranscription regulatory domain removedPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
PTPN13_PDZ_3
Plasmid#103900PurposeProtein expression and purification of PTPN13 PDZ3 domainDepositorInsertMAGI1 (MAGI1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
DLG1_PDZ_1
Plasmid#103915PurposeProtein expression and purification of DLG1 PDZ1 domainDepositorInsertDLG1 (DLG1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAGI1_PDZ_2
Plasmid#103899PurposeProtein expression and purification of MAGI1 PDZ2 domainDepositorInsertMAGI1 (MAGI1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S831E
Plasmid#91997PurposeExpresses FLAG-HA-AGO2 (S831E)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS831EPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-DEST-p110 CUX1
Plasmid#100816PurposeGateway lentiviral vector expressing p100 CUX1 (amino acids 747-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tagDepositorInsertHs CUX1 amino acids 747-1505 of P39880 (CUX1 Human)
UseLentiviralTagsHA and mycExpressionMammalianMutationcontains amino acids 747-1505PromoterAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1α1.1-FLEX-rc [SomArchon-GFP]
Plasmid#153532PurposeAAV-mediated expression of SomArchon-GFP under the EF1α1.1 promoter, in floxed/reversed (Cre-dependent) manner.DepositorInsertSomArchon-GFP
UseAAVTagsER2, GFP, and KGCExpressionMammalianMutationPromoterEF1α1.1Available sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV TIP60 390-513
Plasmid#78797PurposeTo overexpress TIP60 390-513 in Mammalian CellsDepositorInsertTIP60 (KAT5 Human)
UseTags3xFLAGExpressionMammalianMutationaa390-513PromoterCMVAvailable sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
ABE8.13-d
Plasmid#136297Purposeexpresses ABE8.13-d in mammalian cellsDepositorInsertABE8.13-d
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FH-AGO2-S828A
Plasmid#91982PurposeExpresses FLAG-HA-AGO2 (S828A)DepositorInsertAGO2 (AGO2 Human)
UseLentiviralTagsFLAG-HAExpressionMammalianMutationS828APromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-YFP
Plasmid#50483Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
UseTagsYFP fusion in M3-M4 loop (after residue A405)ExpressionMammalianMutationGly-Ala-Gly flexible linker flanking the YFP open…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
USH1C_PDZ_1
Plasmid#103936PurposeProtein expression and purification of P73 PDZ1 domainDepositorInsertUSH1C (USH1C Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
HTRA2_PDZ_1
Plasmid#103913PurposeProtein expression and purification of HTRA2 PDZ domainDepositorInsertHTRA2 (HTRA2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-T830E
Plasmid#91996PurposeExpresses FLAG-HA-AGO2 (T830E)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationT830EPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S834E
Plasmid#91998PurposeExpresses FLAG-HA-AGO2 (S834E)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS834EPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S824A
Plasmid#91987PurposeExpresses FLAG-HA-AGO2 (S824A)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS824APromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-T830A
Plasmid#91988PurposeExpresses FLAG-HA-AGO2 (T830A)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationT830APromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S831A
Plasmid#91989PurposeExpresses FLAG-HA-AGO2 (S831A)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS831APromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S834A
Plasmid#91990PurposeExpresses FLAG-HA-AGO2 (S834A)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS834APromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S828A
Plasmid#91991PurposeExpresses FLAG-HA-AGO2 (S828A)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS828APromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S824E
Plasmid#91994PurposeExpresses FLAG-HA-AGO2 (S824E)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS824EPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S828E
Plasmid#91995PurposeExpresses FLAG-HA-AGO2 (S828E)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS828EPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only
