We narrowed to 4,673 results for: pcr

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pcDNA3_attR1-ORF-attR2-NTEV-TCS-GV-2xHA_DEST

Plasmid

#194385

Purpose

Gateway Destination vector for split TEV assays

Depositor

Michael Wehr

Publication

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

attR1-ORF-attR2-NTEV-TCS-GV-2xHA

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MBP-rPKL WT

Plasmid

#107156

Purpose

MBP-rPKL WT for production and purification of rat PKL WT

Depositor

Jesse Rinehart

Publication

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Promoter

PBAD

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-rPKM2 WT

Plasmid

#107158

Purpose

MBP-rPKM2 WT for production and purification of rat PKM2 WT

Depositor

Jesse Rinehart

Publication

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Promoter

PBAD

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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Sox2-SE-RGM-eGFP targeting vector

Plasmid

#133045

Purpose

Targeting vector of RGM-eGFP with homologous arms around Sox2 super-enhancer DMR

Depositor

Rudolf Jaenisch

Publication

Song et al Mol Cell. 2019 Aug 15. pii: S1097-2765(1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Sox2-SE-5arm-RGM-Sox2-SE-3arm

Use

Mouse Targeting

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pInducer20 2xHA GPR153

Plasmid

#226812

Purpose

Tet-inducible lentiviral vector for 2xHA GPR153 expression

Depositor

Ramiro Iglesias-Bartolome

Publication

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

GPR153 (GPR153 Human)

Use

Lentiviral

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_997253.2

Available since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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Hygro-P2A-HA-FKBP-WAPL

Plasmid

#217666

Purpose

Repair template to endogenously tag WAPL with Hygro-P2A-HA-FKBP

Depositor

Daniel Gerlich

Publication

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

WapI (WAPL Human)

Tags

dTag (FKBP36V protein)

Expression

Bacterial and Mammalian

Promoter

Lac

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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LPAR4-DuET

Plasmid

#213331

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Publication

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LPAR4 (LPAR4 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3_HTR2A-NTEV-TCS-GV-2xHA

Plasmid

#194366

Purpose

Split TEV assays

Depositor

Michael Wehr

Publication

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

HTR2A-NTEV-TCS-GV-2xHA (HTR2A Human, Synthetic)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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miR290-SE-RGM-eGFP targeting vector

Plasmid

#133046

Purpose

Targeting vector of RGM-eGFP with homologous arms around Mir290 super-enhancer DMR

Depositor

Rudolf Jaenisch

Publication

Song et al Mol Cell. 2019 Aug 15. pii: S1097-2765(1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

miR290-SE-5arm-RGM-miR290-SE-3arm

Use

Mouse Targeting

Expression

Mammalian

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pDisplay-CMV-GRAB_LoX3-1.0

Plasmid

#234650

Purpose

This plasmid encodes a genetically encoded fluorescent biosensor based on human CXCR3 for chemokine CXCL9-11 detection.

Depositor

Miao Jing

Publication

Xi et al Immunity. 2025 Sep 9;58(9):2320-2335.e9.

Insert

GPCR activation based chemokine CXCL9-11 sensor GRAB_LoX3-1.0 (CXCR3 Human)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer20 2xHA GPR137

Plasmid

#226813

Purpose

Tet-inducible lentiviral vector for 2xHA GPR137 expression

Depositor

Ramiro Iglesias-Bartolome

Publication

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

GPR137 (GPR137 Human)

Use

Lentiviral

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_001164351

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRF2 CTFdeltaTA-FLAG

Plasmid

#217728

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Publication

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ADGRF2 (ADGRF2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-435

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-hSyn-Galpha-s-IRESWT-Gbetagamma ONE-GO

Plasmid

#204342

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Publication

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-s/Gbetagamma ONE-GO

Use

Lentiviral

Available since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-hSyn-Galpha-i3-IRESWT-Gbetagamma ONE-GO

Plasmid

#204343

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Publication

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-i3/Gbetagamma ONE-GO

Use

Lentiviral

Available since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3_attR1-ORF-attR2-V2R-NTEV-TCS-GV-2xHA_DEST

Plasmid

#194386

Purpose

Gateway Destination vector for GPCR split TEV assays with V2R tail

Depositor

Michael Wehr

Publication

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

attR1-ORF-attR2-V2R-NTEV-TCS-GV-2xHA

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2 ECD

Plasmid

#113954

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 extracellular domain with signal peptide of influenza hemagglutinin

Depositor

Erik Procko

Publication

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

residues 22-568

Promoter

cmv

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR61

Plasmid

#106027

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR61; Opto-GPR61; C5)

Depositor

Harald Janovjak

Publication

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Opto-GPR61 (GPR61 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 RL3m6L2

Plasmid

#109366

Purpose

Human rod opsin chimera with intracellular loop 3 replaced by intracellular loop 2 of mGluR6 with 1D4 tag

Depositor

Robert Lucas

Publication

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rhodopsin chimera with mGluR6 intracellular loop 2 at intracellular loop 3 position (GRM6, RHO Human)

Tags

1D4

Expression

Mammalian

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pInducer20 2xHA LTB4R

Plasmid

#226810

Purpose

Tet-inducible lentiviral vector for 2xHA LTB4R expression

Depositor

Ramiro Iglesias-Bartolome

Publication

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

LTB4R (LTB4R Human)

Use

Lentiviral

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_858043.1

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-PdCO-EGFP

Plasmid

#198512

Purpose

Expresses optimized PdCO in frame with EGFP under control of CMV promotor.

Depositor

Ofer Yizhar

Publication

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PdCO

Tags

EGFP and Rho1D4

Expression

Mammalian

Promoter

CMV

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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