We narrowed to 18,311 results for: puro

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pSLQ7501 pHR (hU6-crNT-EFS-PuroR-WPRE)

Plasmid

#214876

Purpose

Lentiviral vector encoding RfxCas13d nontargeting control guide

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crNT-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_HA-tNGFR_PuroR

Plasmid

#212652

Purpose

piggyBac vector to express HA-tNGFR with puromycin resistance

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

tNGFR

Use

Piggybac

Tags

HA tag

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBabepuro-IL8

Plasmid

#189608

Purpose

Retroviral expression vector for IL8

Depositor

Thierry Tchenio

Insert

IL8 (CXCL8 Human)

Use

Retroviral

Promoter

SV40 early promoter

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-4493NES

Plasmid

#175811

Purpose

The lentiviral vector for Booster-PKA, a Red-Shifted genetically encoded FRET biosensor for monitoring PKA activity.

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

Booster-PKA

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEX-A-U6-MaSgRNA_PuroR

Plasmid

#84780

Purpose

Expresses major satellite-specific sgRNA.

Depositor

Heinrich Leonhardt

Article

Schmidtmann et al Nucleus. 2016 Sep 27:0.

Insert

major satellite-specific sgRNA

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

July 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-mCerulean3

Plasmid

#96936

Purpose

Retroviral plasmid containing mCerulean3 for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Monomeric Cerulean 3

Expression

Mammalian

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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ST-SBP-mCherry_puromycin

Plasmid

#65271

Purpose

ST reporter only ( no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated sialyltransferase (ST6GAL1 Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ARAF_S214C

Plasmid

#51077

Purpose

mammalian expression of ARAF S214C

Depositor

Matthew Meyerson

Article

Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

Insert

ARAF (ARAF Human)

Use

Retroviral

Expression

Mammalian

Mutation

S214C

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pDESTFLAGHApuro3-Ago2K570A

Plasmid

#14102

Depositor

Thomas Tuschl

Article

Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

Insert

Ago2 (AGO2 Human)

Tags

FLAG/HA

Expression

Mammalian

Mutation

changed Lys570 to Ala

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
LncRNA overexpression EF1α-BbsI-SV40polyA_puroR

Plasmid

#219826

Purpose

Overexpression vector for non-coding RNAs driven by EF1α promoter with puromycin resistance. Cloning using BbsI.

Depositor

Philipp Maass

Type

Empty backbone

Expression

Mammalian

Promoter

EF1a

Available Since

July 29, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-EF1a-Ngn3ER-PuroR-mCherry

Plasmid

#197385

Purpose

To establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)

Depositor

Qiao Zhou

Article

Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

Insert

Ngn3ER-2A-PuroR-2A-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin

Plasmid

#115519

Purpose

To construct multiple sgRNA expression vector for CRISPR

Depositor

Xingxu Huang

Insert

ccdB-sgRNA scaffold

Use

CRISPR

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-AREG-ScNeo

Plasmid

#209897

Purpose

To monitor the status of Amphiregulin, the plasmid encodes a recombinant AREG fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

AREG-ScNeo (AREG Human)

Use

Lentiviral

Tags

mNeonGreen and mScarlet

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-NRG1-ScNeo

Plasmid

#209900

Purpose

To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

NRG1-ScNeo (NRG1 Human)

Use

Lentiviral

Tags

mNeonGreen and mScarlet

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH

Plasmid

#225475

Purpose

Express mEGFP-fusion protein of GAPDH

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH (GAPDH Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-OgNLuc_SV40:PuroR

Plasmid

#183038

Purpose

Expresses the FLAG-tagged OgNLuc (Orange LumiFluor) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

OgNLuc LumiFluor

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR-U6gRNA-PGKpuro2ABFP

Plasmid

#136405

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Mutation

BbsI restriction site at position 437 was removed…

Promoter

U6, PDK

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR-U6gRNA-PGKpuro2APDGFB

Plasmid

#136409

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Mutation

BbsI restriction site in the PDGFB sequence (Glu1…

Promoter

U6, PDK

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pT2Apuro-TRE-synPCB2.0

Plasmid

#139481

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7

Use

Tol2 transposon donor vector

Expression

Mammalian

Promoter

Tet responsive element

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ERBB2 C311S

Plasmid

#40985

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

C311S

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only