We narrowed to 17,384 results for: puromycin

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pSB-CMV-MCS-Puro cPLA2-mK2-P2A-EGFP-LaminB1

Plasmid

#162566

Purpose

Used for the dual color labeling of cPLA2 and LaminB1 expression.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Inserts

cytosolic phospholipase a2 (pla2g4aa Zebrafish)
Lamin B1 (lmnb1 Zebrafish)

Tags

EGFP, P2A, and mKate2

Expression

Mammalian

Promoter

CMV and SV40

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-RT004-LaG30VB

Plasmid

#163384

Purpose

2nd generation lentiviral vector to express anti-GFP(LaG30) module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

LaG30-VEEGFRtm-BFP (LaG30VB)

Use

Lentiviral

Tags

BFP

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgSLC7A11/xCT-2

Plasmid

#161819

Purpose

knock out SLC7A11/xCT in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

Insert

solute carrier family 7 member 11 (SLC7A11 Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-BLRR

Plasmid

#158958

Purpose

Bioluminescent repair reporter (BLRR) for DNA double strand break (DSB) repairs: homology-directed repair (HDR) and non-homologous end joining (NHEJ).

Depositor

Charles P. Lai

Article

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

BLRR

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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NINJA TC

Plasmid

#159274

Purpose

Expresses the NINJA targeting construct (inducible neoantigen expression via cumulative Cre, rtTA, doxycycline and tamoxifen).

Depositor

Nikhil Joshi

Article

Damo et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

NINJA TC

Use

Mouse Targeting

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE

Plasmid

#139094

Purpose

Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

KRAB-dSpCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A, H840A

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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PLKO.1-G3BP1-CDS

Plasmid

#136039

Purpose

G3BP1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CGGGAATTTGTGAGACAGTAT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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TPC1-mCherry

Plasmid

#135182

Purpose

Expresses TPC1 with C-terminus mCherry tag in mammalian cells

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TPC1 (Tpcn1 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ-mgU2-47-Mango II

Plasmid

#127586

Purpose

Expresses mgU2-47-Mango II scaRNA from a mU6 promoter

Depositor

David Rueda

Article

Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

Insert

mgU2-47 scaRNA with Mango II tag

Expression

Mammalian

Promoter

modified U6

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-puro-2A-BLM

Plasmid

#127641

Purpose

Over-expression of human BLM

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

BLM (BLM Human)

Use

Lentiviral

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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Nme2sgRNA_pLKO

Plasmid

#119926

Purpose

Nme2Cas9 U6-driven sgRNA cassette

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

NmeCas9 sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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PB-EEA-5g-OSK2M2L1-PGK-Puro

Plasmid

#102909

Purpose

PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE EF1α dCas9-NED

Plasmid

#109369

Purpose

Lentiviral vector for expressing effector domains fused to dCas9.

Depositor

Antal Kiss

Article

Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

3xHA

Expression

Mammalian

Promoter

EF-1α

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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PresentER-MSIIFFLPL (GFP)

Plasmid

#102946

Purpose

PresentER minigene encoding H2-Kb ligand MSIIFFLPL (GFP)

Depositor

David Scheinberg

Article

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Insert

MSIIFFLPL (Serpinf1 Synthetic, Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIPZ Myc2-mouse TTP WT

Plasmid

#107000

Purpose

Expresses epitope-tagged wild-type murine TTP

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

tristetraprolin (Zfp36 Mouse)

Tags

Double Myc peptide epitope tag

Expression

Mammalian

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTRmCerulean3

Plasmid

#90232

Purpose

Lentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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HSPA8_pLX307

Plasmid

#98343

Purpose

Lentiviral expression of HSPA8

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

HSPA8 (HSPA8 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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MTCH2_pLX307

Plasmid

#98354

Purpose

Lentiviral expression of MTCH2

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

MTCH2 (MTCH2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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8027_GFP-KrasG12C_2B_puro

Plasmid

#64372

Purpose

This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

mouse Kras2B (Kras Mouse)

Use

Retroviral

Tags

GFP

Mutation

changed glycine 12 to cysteine

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLIX-REST

Plasmid

#91896

Purpose

Doxycycline inducible expression of mouse REST

Depositor

Julien Sage

Insert

REST (Rest Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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