We narrowed to 13,043 results for: SHI

1. pBS-KS-attB2-SA(1)-T2A-p65AD-Hsp70

   Plasmid

   #62914

   Purpose

   Creating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-p65AD-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mCit-APPAPAPPAP-gs

   Plasmid

   #228091

   Purpose

   Mammalian expression of 10 coiled coil segments in APPAPAPPAP arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cells

   Depositor

   Roman Jerala

   Article

   Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

   Insert

   mCit, APPAPAPPAP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FLAG-ELL pBacPAK8

   Plasmid

   #15322

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

   Insert

   eleven nineteen lysine rich in leukemia (ELL Human)
   

   Use

   Baculovirus construction

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

4. PGK+ plasmid with onboard mCherry cassette

   Plasmid

   #169745

   Purpose

   PGK+ plasmid that also encodes separate mCherry cassette. mCherry is used to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK-tetO2

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. DYSF-Venus

   Plasmid

   #29768

   Depositor

   Steven Vogel

   Article

   Covian-Nares et al J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-

   Insert

   DYSF-Venus (DYSF Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 3, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pmiR-report-hVEGFA

   Plasmid

   #112553

   Purpose

   3'UTR reporter assay

   Depositor

   Shusheng Wang

   Article

   Zhou et al Mol Ther. 2016 Oct;24(10):1823-1835. doi

   Insert

   VEGFA (VEGFA Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS-HsProm1s1-StayGold

   Plasmid

   #211333

   Purpose

   Vector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (WT)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Prominin 1 (PROM1 Human)
   

   Use

   AAV

   Tags

   StayGold

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS MCK promoter

   Plasmid

   #12528

   Depositor

   Ronald Kahn

   Article

   Bruning et al Mol Cell. 1998 Nov . 2(5):559-69.

   Insert

   MCK promoter (Ckm Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPBpuro-miRFP670-HRasCT

   Plasmid

   #176030

   Purpose

   Encoding membrane-targeted miRFP670

   Depositor

   Michiyuki Matsuda

   Article

   Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

   Insert

   Membrane-targeted miRFP670
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLYS5-MCU-Flag

    Plasmid

    #50054

    Purpose

    Expression of human MCU in mammalian cells

    Depositor

    Vamsi Mootha

    Article

    Sancak et al Science. 2013 Nov 14.

    Insert

    MCU-Flag (MCU Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJFRC-nSyb-IVS-PhiC31

    Plasmid

    #133868

    Purpose

    Replace the Nsyb promoter to express PhiC31 under the control of any promoter of interest.

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    PhiC31 Recombinase (int )
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKLV2-EF1adCas9VP64T2ABsd-W

    Plasmid

    #112919

    Purpose

    Lentiviral vector expressing Lentiviral vector expressing Lentiviral vector expressing dCas9VP64 under the control of the long human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    dCas9VP64-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mTFP1-Vinculin-23

    Plasmid

    #55516

    Purpose

    Localization: Focal Adhesions, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

14. CMV-G-GECO1.0

    Plasmid

    #32447

    Purpose

    Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.0)

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    G-GECO1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3 K119I/L173Q/S404G/E430V

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRT498-OtUBD

    Plasmid

    #190089

    Purpose

    Expresses a single OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

    Depositor

    Mark Hochstrasser

    Article

    Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

    Insert

    OtUBD
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTRY-GFP

    Plasmid

    #15301

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    hrGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pcdna3 flag - ULK2 wt

    Plasmid

    #27637

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse ULK2 (Ulk2 Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Initial M removed from ULK2.

    Promoter

    Available since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

18. gh171

    Plasmid

    #106832

    Purpose

    expression of gRNA targeting UGCG

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    UGCG (UGCG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. lentiGuide-RRBP1-1

    Plasmid

    #92156

    Purpose

    CRISPR guide RNA targeting human RRBP1

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    RRBP1 sgRNA-1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NFAST(low)-mApple-6xHp

    Plasmid

    #214412

    Purpose

    Lipid droplet localization of low-affinity NFAST fused to mApple-6xHp

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspA-N
    

    Use

    Tags

    mApple-6xHp

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUC18T-mini-Tn7T-Gm-Pc-mTurquoise2

    Plasmid

    #111259

    Purpose

    mini-Tn7 vector for insertion of mTurquoise2 (codon optimized for P. fluorescens) at bacterial attTn7 site

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mTurquoise2 is codon optimized for expression in …

    Promoter

    Pc

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pENTR223-BORF1

    Plasmid

    #195804

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BORF1

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BORF1
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pX459V2.0-SpCas9-HF1

    Plasmid

    #108293

    Purpose

    pX459 V2.0 (Plasmid #62988) with the N497A, R661A, Q695A, and Q926A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCS2TAL3-DD

    Plasmid

    #37275

    Purpose

    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)

    Depositor

    David Grunwald

    Article

    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

    Type

    Empty backbone

    Use

    Tags

    Flag, Fok I DD, Tal-C, and Tal-N

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. HA-E2F-1 wt-pRcCMV

    Plasmid

    #21667

    Depositor

    William Kaelin

    Article

    Krek et al Cell. 1994 Jul 15. 78(1):161-72.

    Insert

    E2F-1 (E2F1 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wild type-

    Promoter

    Available since

    Sept. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti-NG-PE2-BSD

    Plasmid

    #176933

    Purpose

    Expresses PE2-NG variant in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

    Insert

    PE2-NG variant
    

    Use

    Lentiviral

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBS-KS-attB2-SA(1)-T2A-3X Gal80-Hsp70

    Plasmid

    #62952

    Purpose

    Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    3XGal80-FLAG/HA/EGFP-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLYS1-EMRE/RNAi resistant

    Plasmid

    #50056

    Purpose

    Expression of human EMRE in mammalian cells with silent mutation making it resistant to RNAi

    Depositor

    Vamsi Mootha

    Article

    Sancak et al Science. 2013 Nov 14.

    Insert

    EMRE-RNAi resistant (SMDT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    RNAi resistant, silent mutant

    Promoter

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CMV-G-GECO1.1

    Plasmid

    #32445

    Purpose

    Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.1)

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    G-GECO1.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBS-KS-attB2-SA(0)-T2A-LexA::QFAD-Hsp70

    Plasmid

    #62947

    Purpose

    Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-LexA::QFAD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pILGFP92

    Plasmid

    #83549

    Purpose

    Used to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TDH3 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1-hAsCpf1(TYCV) (pY210)

    Plasmid

    #89351

    Purpose

    Expresses humanized AsCpf1 variant that recognizes TYCV PAMs

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1, RR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    S542R/K607R

    Promoter

    CMV

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pETM6-G6-vioABECD

    Plasmid

    #66536

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with G6 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Mutant T7 Promoter - G6

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pENTR223-BNRF1

    Plasmid

    #198580

    Purpose

    Gateway Entry Clone

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BNRF1 (BNRF1 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    None (wildtype)

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pClneoMyc mouse LNX2

    Plasmid

    #37010

    Depositor

    Yutaka Hata

    Article

    Kansaku et al Oncogene. 2006 Aug 24;25(37):5071-84. Ep

    Insert

    LNX2 (Lnx2 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2012

    Availability

    Academic Institutions and Nonprofits only

36. pMXs-Scl

    Plasmid

    #131601

    Purpose

    expression of mouse ScI in mammalian cells

    Depositor

    Kateri Moore, Filipe Pereira

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    Scl (Tal1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHLmMBP-1

    Plasmid

    #72343

    Purpose

    Mammalian expression of secreted mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Use

    Tags

    6His-tag, HA-tag, Strep-tag II, and mMBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. lentiGuide-RRBP1-2

    Plasmid

    #92157

    Purpose

    CRISPR guide RNA targeting human RRBP1

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    RRBP1 sgRNA-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pT-GEM(0)

    Plasmid

    #62891

    Purpose

    Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pENTR223-BMRF1(EA-D)

    Plasmid

    #195807

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BMRF1

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BMRF1 (BMRF1 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. IMS-HyPer2

    Plasmid

    #60248

    Purpose

    Genetically-encoded fluorescent hydrogen peroxide indicator HyPer2 targeted to mitochondrial intermembrane spase.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    IMS-HyPer2
    

    Use

    Tags

    Mitochondrial intermembrane space targeting signa…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

    Plasmid

    #159803

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pJFRC-20XUAS-IVS-PhiC31

    Plasmid

    #133565

    Purpose

    Replace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest.

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    PhiC31 Recombinase (int )
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    20X UAS

    Available since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3

    Plasmid

    #62957

    Purpose

    Triplet donor for in vivo RMCE with MiMIC inserts to make Gal4 driver.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4 in three phases
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pJL1 Aquamarine

    Plasmid

    #106285

    Purpose

    Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    Aquamarine
    

    Use

    Tags

    6xHis and TEV cleavage (N terminal on insert)

    Expression

    Bacterial

    Mutation

    T65S and H148G

    Promoter

    T7

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCMV-CMP-PE-V1

    Plasmid

    #202769

    Purpose

    Expresses CMP-PE-V1 in mammalian cells for prime editing

    Depositor

    Kyoungmi Kim

    Article

    Park et al Genome Biol. 2021 Jun 3;22(1):170. doi:

    Insert

    CMP-PE-V1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pGEX-4T-2/hGGA3(GAT)

    Plasmid

    #79436

    Purpose

    Expresses GST-tagged GAT domain of GGA3 (107-286 aa) in E.coli

    Depositor

    Kazuhisa Nakayama

    Article

    Takatsu et al Biochem J. 2002 Jul 15;365(Pt 2):369-78.

    Insert

    GGA3 (GGA3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 107-286

    Promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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48. pEGFP-N2-RIPK3

    Plasmid

    #78822

    Purpose

    To detect localization of RIPK3 protein in live mammalian cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

49. pBS-KS-attB2-SA(0)-T2A-Gal4-Hsp70

    Plasmid

    #62896

    Purpose

    Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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50. pcDNA3.1hSIRT4_HA

    Plasmid

    #24482

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Sirtuin 4 (SIRT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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