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Showing: 7801 - 7850 of 24790 results

  1. PLJR965_Rv2464c

    Plasmid
    #241232
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv2464c) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv2464c sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241232-plasmid-map-sequence-id-483381

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  2. PLJR965_Rv1638

    Plasmid
    #241231
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv1638) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv1638 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241231-plasmid-map-sequence-id-483389

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  3. PLJR965_Rv1259

    Plasmid
    #241228
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv1259) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv1259 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241228-plasmid-map-sequence-id-483385

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  4. PLJR965_Rv1020

    Plasmid
    #241227
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv1020) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv1020 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241227-plasmid-map-sequence-id-483388

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  5. PLJR965_Rv0949

    Plasmid
    #241226
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv0949) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv0949 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241226-plasmid-map-sequence-id-483386

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  6. PLJR965_Rv1633

    Plasmid
    #241230
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv1633) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv1633 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    241230-plasmid-map-sequence-id-483390

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  7. PLJR965_Rv3589

    Plasmid
    #241236
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv3589) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv3589 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    241236-plasmid-map-sequence-id-483387

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  8. PLJR965_Rv2924c

    Plasmid
    #241233
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv2924c) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv2924c sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    241233-plasmid-map-sequence-id-483382

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  9. PLJR965_Rv1420

    Plasmid
    #241229
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv1420) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv1420 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    241229-plasmid-map-sequence-id-483380

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  10. 1203AB-CMV-Cas7.1-H43A-mutant

    Plasmid
    #221541
    Purpose
    Effector expresses Cas7.1 (truncated from DiCas7-11), H to A mutation at amino acid 43
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221541-plasmid-map-sequence-id-437821

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  11. 1203AC-CMV-Cas7.1-Y55A-mutant

    Plasmid
    #221542
    Purpose
    Effector expresses Cas7.1 domain (truncated from DiCas7-11), Y to A mutation at amino acid 55
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221542-plasmid-map-sequence-id-437820

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  13. pFETCh_TGIF2

    Plasmid
    #72373
    Purpose
    Donor vector for 3' FLAG tag of human TGIF2
    Depositor
    Eric Mendenhall, Richard M. Myers
    Insert
    TGIF2 Homology Arms (TGIF2 Human)
    Use
    CRISPR
    Tags
    3XFLAG-P2A-NeoR
    Available Since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72373-plasmid-map-sequence-id-132123

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  15. 1203N-CMV-dDiCas7-11-NLS-bGHpolyA

    Plasmid
    #221537
    Purpose
    Deactivated DiCas7-11 effector with CMV promoter/enhancer and nuclear localization signal sequence at 5' and 3' end of the coding domain
    Depositor
    Omar Akbari
    Insert
    DiCas7-11
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221537-plasmid-map-sequence-id-437448

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  16. 1203AD-CMV-Cas7.1-N152A-mutant

    Plasmid
    #221543
    Purpose
    ffector expresses Cas7.1 domain (truncated from DiCas7-11), N to A mutation at amino acid 152
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221543-plasmid-map-sequence-id-437816

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  17. pTargetAmp

    Plasmid
    #228552
    Purpose
    Template for PCR amplification for gRNA cloning plasmid by in vivo cloning.
    Depositor
    Jeferson Gross
    Insert
    Ampicillin resistance
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228552-plasmid-map-sequence-id-454861

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  18. pR6K-GFP-CASTIB

    Plasmid
    #199655
    Purpose
    R6K plasmid with a I-B CAST but no CRISPR array
    Depositor
    IIya Finkelstein
    Insert
    CAST I-B systems
    Expression
    Bacterial
    Promoter
    J23119 promoter
    Available Since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199655-plasmid-map-sequence-id-412259

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  20. pX330-Flag-HFSpCas9-H840A

    Plasmid
    #80455
    Purpose
    high fidelity SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    HFSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80455-plasmid-map-sequence-id-151476

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  21. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid
    #195316
    Purpose
    Dox inducible lentiviral dead Cas9
    Depositor
    Raj Chari
    Insert
    dead Cas9
    Use
    CRISPR and Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    D10A; H840A
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    195316-plasmid-map-sequence-id-385750

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  22. pR6K-GFP-CASTIF

    Plasmid
    #199653
    Purpose
    R6K plasmid encoding a type I-F CAST but no CRISPR array
    Depositor
    IIya Finkelstein
    Insert
    CAST I-F systems
    Expression
    Bacterial
    Promoter
    J23119 promoter
    Available Since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199653-plasmid-map-sequence-id-412253

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  23. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid
    #106477
    Purpose
    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106477-plasmid-map-sequence-id-207983

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  24. pFETCh_SSRP1

    Plasmid
    #72370
    Purpose
    Donor vector for 3' FLAG tag of human SSRP1
    Depositor
    Eric Mendenhall, Richard M. Myers
    Insert
    SSRP1 Homology Arms (SSRP1 Human)
    Use
    CRISPR
    Tags
    3XFLAG-P2A-NeoR
    Available Since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72370-plasmid-map-sequence-id-132120

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  25. GuideVector_BbsI

    Plasmid
    #110692
    Purpose
    For rapid cloning and expression of sgRNAs in mammalian cells. Contains copGFP for easy FACS sorting.
    Depositor
    Douglas Mahoney
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    May 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    110692-plasmid-map-sequence-id-211319

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  26. pJJR81

    Plasmid
    #75029
    Purpose
    tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Mike Boxem
    Insert
    tagBFP2^SEC^3xFlag
    Use
    CRISPR
    Tags
    3xFLAG and C. elegans codon optimized tagBFP2
    Expression
    Worm
    Available Since
    June 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    75029-plasmid-map-sequence-id-141917

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  28. pFETCh_ZNF3

    Plasmid
    #72362
    Purpose
    Donor vector for 3' FLAG tag of human ZNF3
    Depositor
    Eric Mendenhall, Richard M. Myers
    Insert
    ZNF3 Homology Arms (ZNF3 Human)
    Use
    CRISPR
    Tags
    3XFLAG-P2A-NeoR
    Available Since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    72362-plasmid-map-sequence-id-132112

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  29. pX330-Flag-HeFSpCas9-H840A

    Plasmid
    #80456
    Purpose
    high fidelity enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    HeFSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Aug. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80456-plasmid-map-sequence-id-151478

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  30. pYJ15-PB-U6-Nkx6.1-acti-gRNA1

    Plasmid
    #131059
    Purpose
    Activation of NKX6.1 via SAM system
    Depositor
    Xiaojun Lian
    Insert
    NKX6.1 activation gRNA1 (NKX6-1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131059-plasmid-map-sequence-id-256775

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  31. pYJ16-PB-U6-Nkx6.1-acti-gRNA2

    Plasmid
    #131060
    Purpose
    Activation of NKX6.1 via SAM system
    Depositor
    Xiaojun Lian
    Insert
    NKX6.1 activation gRNA2 (NKX6-1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131060-plasmid-map-sequence-id-256750

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  32. pYJ17-PB-U6-Nkx6.1-acti-gRNA3

    Plasmid
    #131061
    Purpose
    Activation of Mafa via SAM system
    Depositor
    Xiaojun Lian
    Insert
    NKX6.1 activation gRNA3 (NKX6-1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131061-plasmid-map-sequence-id-256770

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  33. pYJ27-AAVS1-XLone-NKX6.1

    Plasmid
    #131070
    Purpose
    Donor plasmid of targeted inducible-NKX6.1 knockin
    Depositor
    Xiaojun Lian
    Insert
    NKX6.1 (NKX6-1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131070-plasmid-map-sequence-id-256822

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  34. 1203T-CMV-Cas7.1-NLS-bGHpolyA

    Plasmid
    #221540
    Purpose
    Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector), nuclear localization signal sequence on 5' and 3' end of the coding domain
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221540-plasmid-map-sequence-id-437823

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  35. 1203W-CMV-Cas7.1-7.2-bGHpolyA

    Plasmid
    #220961
    Purpose
    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and Cas7.2 domains from DiCas7-11, deactivated (truncated from DiCas7-11 effector)
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    220961-plasmid-map-sequence-id-437427

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  36. pR6K-GFP-CASTV

    Plasmid
    #199657
    Purpose
    R6K plasmid encoding a type V CAST but no CRISPR array
    Depositor
    IIya Finkelstein
    Insert
    CAST V systems
    Expression
    Bacterial
    Promoter
    J23119 promoter
    Available Since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199657-plasmid-map-sequence-id-412267

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  37. pTD56

    Plasmid
    #172895
    Purpose
    mKate2^SEC^AID* vector with ccdB markers for cloning homology arms
    Depositor
    David Reiner
    Insert
    mKate2
    Use
    CRISPR
    Available Since
    Feb. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    172895-plasmid-map-sequence-id-353627

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  38. pTD72

    Plasmid
    #172896
    Purpose
    mKate2^SEC^2xHA vector with ccdB markers for cloning homology arms
    Depositor
    David Reiner
    Insert
    mKate2
    Use
    CRISPR
    Available Since
    Feb. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    172896-plasmid-map-sequence-id-353630

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  39. pML104-KanMx4

    Plasmid
    #83476
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    KanMX4
    Expression
    Yeast
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    83476-plasmid-map-sequence-id-189195

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  40. pMV_AA098

    Plasmid
    #245320
    Purpose
    Cas9 CRISPRko all-in-one positive control; targets CD47
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD47 (CD47 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245320-plasmid-map-sequence-id-488052

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  41. pPB-US-ECasE

    Plasmid
    #83961
    Purpose
    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.
    Depositor
    Eleanor Chen
    Insert
    ERT2-spCas9-ERT2
    Expression
    Mammalian
    Mutation
    please see depositor comments below
    Promoter
    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83961-plasmid-map-sequence-id-214594

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  42. pML104-HygMx4

    Plasmid
    #83475
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    HphMX4
    Expression
    Yeast
    Available Since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    83475-plasmid-map-sequence-id-358352

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  43. pGW_RUBY_EggCas9

    Plasmid
    #225990
    Purpose
    To clone entry vector with CRISPR guide RNA via Gateway cloning; it has an Egg cell-specific expression of Cas9 and the plant is selected by the red color due to constitutive expression of RUBY
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    225990-plasmid-map-sequence-id-448019

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  44. lenti-dCas9-VP64-BFP

    Plasmid
    #196711
    Purpose
    CRISPR-activation. dCas9-VP64 with TagBFP for sorting
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-VP64-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available Since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    196711-plasmid-map-sequence-id-392561

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  45. lenti-UCOE-dCas9-BFP-VP64

    Plasmid
    #196717
    Purpose
    CRISPR-activation. dCas9-VP64 with TagBFP for sorting.
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-VP64
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196717-plasmid-map-sequence-id-388038

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  46. pML104-NatMx3

    Plasmid
    #83477
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    NatMx3
    Expression
    Yeast
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only

  48. pAAV-dSa-VPR

    Plasmid
    #99651
    Purpose
    Expresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9
    Depositor
    George Church
    Insert
    dCas9
    Use
    AAV
    Tags
    VP64-p65-RTA activator
    Mutation
    dead Cas9
    Available Since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    99651-plasmid-map-sequence-id-191797

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Showing: 7801 - 7850 of 24790 results