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We narrowed to 18,247 results for: multi

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Showing: 3121 - 3140 of 18247 results

  5. pTUB1:OsTIR1-3FLAG, SAG1:CAT

    Plasmid
    #87258
    Purpose
    TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.
    Depositor
    David Sibley
    Publication
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    TIR1
    CAT
    Use
    Toxoplasma expression
    Tags
    3FLAG
    Mutation
    Codon optimized for Toxoplasma gondii expression
    Promoter
    TgSAG1 361 bp and TgTUB1 2717 bp
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87258-plasmid-map-sequence-id-176556

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  6. pHL-H1-ccdB-mEF1a-RiH

    Plasmid
    #60601
    Purpose
    Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.
    Depositor
    Akitsu Hotta
    Publication
    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Red Fluorescent Protein
    Hygromycin resistance gene
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60601-plasmid-map-sequence-id-97111

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  10. HSC1-HS4-GiP

    Plasmid
    #58540
    Purpose
    Derived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTR
    Depositor
    James Ellis
    Publication
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Core HS4 insulator sequence (dimer)
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    Human EF1-alpha
    Available since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58540-plasmid-map-sequence-id-90629

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  11. pAC149-pCR8-dCas9VP160

    Plasmid
    #48221
    Purpose
    dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain
    Use
    CRISPR and Gateway Cloning
    Tags
    HA Tag and VP160
    Mutation
    D10A;H840A nuclease-deficient
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48221-plasmid-map-sequence-id-69506

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  15. pNTI194

    Plasmid
    #84266
    Purpose
    Expresses Avi-tagged RPL1 and humanized birA
    Depositor
    Nicholas Ingolia
    Publication
    Ingolia et al Cell Rep. 2014 Sep 11;8(5):1365-79. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    RPL1 (RPL10A Human, (*see below))
    humanized birA
    Tags
    Avi tag, Flag, Gly/Ser linker, and TEV site
    Expression
    Mammalian
    Mutation
    R33A, A34E (*see below)
    Promoter
    CMV and P2A peptide
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84266-plasmid-map-sequence-id-160813

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  17. AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60225
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA targeting LacZ. AAV backbone.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available since
    Oct. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    60225-plasmid-map-sequence-id-96391

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  18. pHCAG-L2EOP

    Plasmid
    #51783
    Purpose
    The oriP/EBV nuclear antigen (EBNA)-1 elements of Epstein-Barr virus is fused in frame with the luciferase in the herpes simplex virus type-1 amplicon vector, provides prolonged transgene expression
    Depositor
    Paula Lam
    Publication
    Sia et al J Gene Med. 2010 Oct;12(10):848-58. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Luciferase-2A-EBNA-1
    OriP
    Use
    Herpes simplex virus type-1 amplicon vector
    Promoter
    CAG
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    51783-plasmid-map-sequence-id-79421

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  20. pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197061
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Publication
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197061-plasmid-map-sequence-id-402602

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Showing: 3121 - 3140 of 18247 results