We narrowed to 5,328 results for: pcdna3.1

1. pNIR-Fbactin

   Plasmid

   #184677

   Purpose

   NIR-Fb to actin

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   NIR-Fbactin
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. ER2-mhYFP

   Plasmid

   #186528

   Purpose

   Localization and retention of monomeric hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   Monomeric hyperfolder YFP
   

   Tags

   Calreticulin and KDEL

   Expression

   Mammalian

   Mutation

   hfYFP-S147P/L195M/V206K

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-Mirk (pHX9F)

   Plasmid

   #17846

   Depositor

   Eileen Friedman

   Article

   Lee et al Cancer Res. 2000 Jul 1. 60(13):3631-7.

   Insert

   Mirk (DYRK1B Human)
   

   Tags

   6xHis and Anti-XPress

   Expression

   Mammalian

   Available Since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

4. Mammalian_SpCas9_Target-AID3S

   Plasmid

   #188649

   Purpose

   Expresses SpCas9_Target-AID3S in mammalian cells

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Insert

   nCas9-AID3S-UGI
   

   Tags

   UGI

   Expression

   Mammalian

   Mutation

   D10A for SpCas9

   Promoter

   pCMV

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. rArc

   Plasmid

   #178206

   Purpose

   Ratiometric version of the genetically encoded voltage indicator ArcLight-Q239.

   Depositor

   Stefan Heinemann

   Article

   Ruhl et al Commun Biol. 2021 Oct 7;4(1):1164. doi:

   Insert

   rArc
   

   Expression

   Mammalian

   Available Since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-Tubulin.K40Q

   Plasmid

   #32912

   Depositor

   Tso-Pang Yao

   Article

   Gao et al J Biol Chem. 2010 Apr 9. 285(15):11219-2

   Insert

   Tubulin (TUBA1B Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K40Q

   Promoter

   CMV

   Available Since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pZ148-BhCas12b-sgRNA-scaffold

   Plasmid

   #122448

   Purpose

   Expresses sgRNA for BhCas12b in mammalian cells

   Depositor

   Feng Zhang

   Article

   Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

   Insert

   U6-BhCas12b-sgRNA-scaffold, CMV-mCherry
   

   Expression

   Mammalian

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. mito-mGarnet

   Plasmid

   #104309

   Purpose

   codes for a fusion of fluorescent protein mGarnet and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mito

   Depositor

   Ulrich Nienhaus

   Article

   Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

   Insert

   mito-mGarnet
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-LBR-EGFP-polyA

   Plasmid

   #59748

   Purpose

   Express LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   LBR-EGFP
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTS1017-Tier1-OTetO7-PCMVmin-SEAP

    Plasmid

    #169586

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Expression

    Mammalian

    Promoter

    TetO7-PCMVmin

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. YFP Gamma 9

    Plasmid

    #36107

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein subunit Gamma 9
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    HIs tag between YFP and insert

    Promoter

    CMV

    Available Since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

12. GFPxm163

    Plasmid

    #193480

    Purpose

    Turn-off fluorescent sensor for chloride

    Depositor

    Sheel Dodani

    Article

    Peng et al Chem Sci. 2022 Oct 6;13(43):12659-12672.

    Insert

    GFPxm163
    

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMM310-Myc

    Plasmid

    #80053

    Purpose

    Expression of a Myc tagged version of the BlaM-Vpr fusion protein

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    Myc-BlaM-Vpr
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    N-terminal Myc tag added to construct pMM310

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 Plk4(Sak) D154A (Nigg HR26)

    Plasmid

    #41164

    Depositor

    Erich Nigg

    Article

    Habedanck et al Nat Cell Biol. 2005 Nov;7(11):1140-6.

    Insert

    Plk4 D154A (PLK4 Human)
    

    Tags

    3xMyc and PreScission Site

    Expression

    Mammalian

    Mutation

    D154A

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. mNeonGreen-IETD-NanoLuc

    Plasmid

    #98288

    Purpose

    caspase-8 BRET Sensor

    Depositor

    Maarten Merkx

    Article

    den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

    Insert

    mNeonGreen-LEHD-NanoLuc
    

    Tags

    His-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-Golgi-ZapCY1

    Plasmid

    #36322

    Depositor

    Amy Palmer

    Article

    Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

    Insert

    Golgi-ZapCY1 high affinity Zn(II) sensor
    

    Tags

    GalT (first 60 amino acids)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCD/NL-BH*deltavpu/RT-

    Plasmid

    #136985

    Purpose

    Second-generation lentiviral packaging plasmid lacking reverse transcriptase activity

    Depositor

    Jakob Reiser

    Article

    Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

    Insert

    HIV-1 Gag, Pol (Integrase), Tat, Rev, Vpr, Vif
    

    Expression

    Mammalian

    Mutation

    carries D110E mutation in the reverse transcripta…

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-LBR-mRFP1-polyA

    Plasmid

    #59749

    Purpose

    Express LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    LBR-mRFP1
    

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    aa 1-249 of LBR

    Promoter

    CMV and T7

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. FRB-myc

    Plasmid

    #20228

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    FRB (MTOR Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    T2098L point mutation

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pCMV_mCherry_2A_HA_2xMS2-VP64

    Plasmid

    #68419

    Purpose

    Transient expression of humanized MS2 (V75E/A81G), fused to the VP64 transcrption activator, in mammalian cells, under a CMV promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    MS2
    

    Use

    Synthetic Biology

    Tags

    HA and VP64

    Expression

    Mammalian

    Mutation

    MS2: V75E/A81G

    Promoter

    CMV

    Available Since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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