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Plasmid#108244PurposeMembrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expressionDepositorInsertMembrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
UseSynthetic BiologyTagsExpressionMutationPromoterpLacO1Available sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
T7 promoter + terminators reporter plasmid
Plasmid#156456PurposeA mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.DepositorInsertsNeoR/KanR
TcR
UseTagsExpressionBacterialMutationATG start codon mutated to ACGPromoterEm7 and tetAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
mOtop3_pGEMHE
Plasmid#114676PurposeThis construct was used to express mouse Otop3 in Xenopus laevis oocytesDepositorInsertOtopetrin 3 (Otop3 Mouse)
UseFor generating the mrna, which can highly express…TagsExpressionMutationPromoterT7Available sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
shh10
Plasmid#64867PurposeThis plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.DepositorInsertshh10 cap
UseAAVTagsExpressionMutationI319V, N451D, D532NPromoterAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
piggyBAC-5xTAPE-1-BlastR
Plasmid#175808PurposeEncodes 5xTAPE-1 construct that can be integrated using piggyBAC systemDepositorInsert5xTAPE-1 construct
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-C1
Plasmid#60560PurposeExpresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteinsDepositorInsertmTurquoise2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-N1
Plasmid#60561PurposeExpresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteinsDepositorInsertmTurquoise2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
SiC-V1
Plasmid#133041PurposeSiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.DepositorInsertSpCas9
UseCRISPR and LentiviralTagsdTomatoExpressionMutationPromoterAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLC133
Plasmid#198639PurposeExpression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.DepositorInsertdCas9
UseTags3x-FLAGExpressionBacterialMutationPromoterpTetAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCD/NL-BH∆1
Plasmid#41791PurposePackaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding RevDepositorInsertHIV-1 Gag/Pol and RRE sequences
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only