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mCerulean3-Actin-7

Plasmid

#55399

Purpose

Localization: Actin, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Actin (ACTB Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
LB334 (Bcl-2 from -1287 to -1640)

Plasmid

#15241

Depositor

Linda Boxer

Article

Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from -1287 to -1640 with P1

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pLXSN-FLK1 TM

Plasmid

#65249

Purpose

Expression of dominant negative FLK1 in mammalian cells

Depositor

Axel Ullrich

Article

Millauer et al Nature. 1994 Feb 10;367(6463):576-9.

Insert

FLK1 TM (Kdr Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

deleted AA 786-1346

Promoter

LTR

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pM-ErbB4CTF

Plasmid

#17797

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment only, amino acids 676-1…

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Cav1 in situ probe

Plasmid

#45624

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Cav1 in situ probe (Cav1 Mouse)

Use

In situ

Mutation

fragment contains bp# 1472-2124 of AB029929.1

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
-111 hTF m2

Plasmid

#15449

Depositor

Nigel Mackman

Article

Cui et al J Biol Chem. 1996 Feb 2. 271(5):2731-9.

Insert

Tissue factor promoter (F3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

All 3 Sp1 sites mutated

Available Since

Aug. 31, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA1-GATA4-opposite orientation

Plasmid

#51928

Purpose

for T7 directed antisense production of mouse GATA4

Depositor

Jeffery Molkentin

Article

Liang et al J Biol Chem. 2001 Aug 10;276(32):30245-5

Insert

GATA4 reverse orientation (Gata4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2014

Availability

Academic Institutions and Nonprofits only
MAC_C_ERBB2

Plasmid

#187776

Purpose

MAC-tagged gene expression of human ERBB2

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

ERBB2 (ERBB2 Human)

Expression

Mammalian

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_PRKACA_C1+C2

Plasmid

#110027

Purpose

Protein expression and purification of PRKACA_C1+C2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PRKACA_C1+C2 (PRKACA Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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EGFR∆550-580-mEGFP

Plasmid

#203788

Purpose

Number and brightness analysis of EGFR oligomerization

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

EGFR (EGFR Human)

Tags

mEGFP

Expression

Mammalian

Mutation

aa 550-580 deleted

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ERBB4 (E317K)

Plasmid

#29528

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Expression

Mammalian

Mutation

changed glutamic acid 317 to lysine

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pJW1241

Plasmid

#74904

Purpose

Expresses 3XFLAG::GR LBD::eGFP from the hsp16.48 promoter

Depositor

Jordan Ward, Keith Yamamoto

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Insert

3X FLAG::GR LBD::eGFP (NR3C1 Nematode, Human, Synthetic)

Expression

Worm

Promoter

hsp16.48

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Hopx

Plasmid

#74347

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Hopx. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

HOP homeobox (Hopx Mouse)

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFETCh_MED13_iso1

Plasmid

#135733

Purpose

Donor vector for 3' FLAG tag of human MED13_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

MED13_iso1 Homology arms (MED13 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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GST-rhodopsin-CTS

Plasmid

#86071

Purpose

A bacterial expression plasmid encoding rhodopsin ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Rhodopsin (RHO Bovine)

Tags

GST

Expression

Bacterial

Mutation

contains the region from 309-348 of Rhodopsin

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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3542 pMIG Bcl-XL mut1

Plasmid

#8791

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bcl-XL (BCL2L1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

F131V, D133A

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pCDF1-ERBB4 (E836K)

Plasmid

#29542

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pEGFPC1-Intu

Plasmid

#65430

Purpose

For mammalian expression of EGFP-mouse Inturned

Depositor

Aimin Liu

Article

Zeng et al Dev Biol. 2010 Mar 15;339(2):418-28. doi

Insert

Inturned (Intu Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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tdEos-Vimentin-N-18

Plasmid

#57690

Purpose

Localization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

NM_003380.3 (VIM Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCDF1-ERBB4 (E542K)

Plasmid

#29539

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only