We narrowed to 7,885 results for: Lif

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pSMT3-FBF2 AS/Y

Plasmid

#166974

Purpose

Expresses FBF-2 with AS/Y mutations in repeat 5 in E. coli for purification and crystallization studies

Depositor

Traci Hall

Article

Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

Insert

FBF-2 (fbf-2 Nematode)

Tags

His-SUMO

Expression

Bacterial

Mutation

FBF-2 PUM RNA binding domain; amino acids 164-575…

Promoter

T7

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP)

Plasmid

#91806

Purpose

Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

Residues A1529-G1534 replaced with a PreScission …

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP)

Plasmid

#91807

Purpose

Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

PreScission Protease site (LEVLFQGP) inserted bet…

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)

Plasmid

#91818

Purpose

Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutations

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

SPT6 (SPT6 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

changed lysine 1355 and lysine 1435 to alanines; …

Promoter

T7

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP N1 AURKA deltaNter

Plasmid

#157753

Purpose

Expression of AURKA without Nter, fused to GFP in mammalian cells

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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p1A Prk-

Plasmid

#162694

Purpose

p1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

H. neapolitanus CbbLS and S. elongatus Prk

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Mutation

Prk inactive (K20M S21A native protein, this map …

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(T/A)

Plasmid

#123340

Purpose

Negative control mutant for Venus-cpVenus-FLARE-EKAR-EV biosensor.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-EKAR-EV(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-cpVenus-FLARE-CKAR(T/A)

Plasmid

#123344

Purpose

Negative control mutant for Venus-cpVenus-FLARE-CKAR biosensor.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-CKAR(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean3-cpCerulean3-FLARE-AKAR

Plasmid

#123336

Purpose

Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Cerulean3/cpCerulean3-E173 homoFRET pair.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Cerulean3-cpCerulean3-FLARE-AKAR

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-Mad3 [N129A/6R]

Plasmid

#140075

Purpose

Mammalian Expression Plasmid of anti-Mad3 (Human). Derived from hybridoma N129A/6.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Mad3 (Human) recombinant mouse monoclonal antibody (MXD3 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-PTEN-3'UTR

Plasmid

#97204

Purpose

Expresses PTEN 3'UTR in mammalian cells

Depositor

Reproducibility Project Cancer Biology

Article

Kerwin et al Elife. 2020 Apr 21;9. pii: 51019. doi: 1

Insert

PTEN 3' UTR (PTEN Human)

Tags

none

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GABARα6N-HA

Plasmid

#128111

Purpose

Express HA tagged GABARα6 N terminal domain

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Gabra6 (Gabra6 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-12 [N277/7R]

Plasmid

#114482

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-12 (Mouse). Derived from hybridoma N277/7.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Synaptotagmin-12 (Mus musculus) recombinant mouse monoclonal antibody (Syt12 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJET1.2_Bod1l_Sense

Plasmid

#124443

Purpose

Plasmid for sense in situ probe in vitro transcription

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Insert

Biorientation of chromosomes in cell division 1-like (Bod1l Mouse)

Use

In situ probe

Promoter

T7

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-Hemmar-DSCP-ftz-FLP(D)

Plasmid

#119894

Purpose

FLP(D) with a Drosophila Synthetic Core Promoter (DSCP) and ftz intron

Depositor

Rita Sousa-Nunes

Article

Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

Inserts

FLP(D)
DSCP-ftz (ftz Fly)

Use

Drosophila expression

Mutation

a mutated form of FLP, which at position 5 contai…

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral N

Plasmid

#113009

Purpose

For bacterial expression of MBP fusion of N terminal region of Drosophila Tral

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

N terminus of tral (tral Fly)

Expression

Bacterial

Mutation

amino acids 1-355

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral FL-15A

Plasmid

#113011

Purpose

For bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length tral (tral Fly)

Expression

Bacterial

Mutation

15 PNG phosphorylation sites mutated to A

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral C

Plasmid

#113008

Purpose

For bacterial expression of MBP fusion of C terminal region of Drosophila Tral

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

C terminus of tral (tral Fly)

Expression

Bacterial

Mutation

amino acids 356-652

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral C 15A

Plasmid

#113007

Purpose

For bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A)

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

C terminus of tral (tral Fly)

Expression

Bacterial

Mutation

PNG phosphorylation sites mutated to A

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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Lrrc4c-AP-His

Plasmid

#71959

Purpose

Expresses the extracellular region of the LRRC4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Lrrc4c (Lrrc4c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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