We narrowed to 5,487 results for: chia

1. pNB_EA_R1CiM_4EGFP_R3EGFPi2

   Plasmid

   #185968

   Purpose

   Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.

   Depositor

   Sangram Bagh

   Article

   Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

   Insert

   Molecular IMPLY gate
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNB_EA_R3tdTomatoi2

   Plasmid

   #185969

   Purpose

   Expresses tdTomato from PR promoter with optimized RBS (i2) in E.coli Dh5alpha cell. The construct is in reverse direction network brick.

   Depositor

   Sangram Bagh

   Article

   Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

   Insert

   Pr-i2rbs-tdTomato cassette in reverse direction in Network Brick
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PR

   Available since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUPD2 PhiC31 integrase (GB1561)

   Plasmid

   #160586

   Purpose

   Streptomyces phage PhiC31 integrase, adapted for C-terminal fusions

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   PhiC31
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-HA-GlpG(D243G/L244G/F245G/M247G/S248G/M249G/A250G)

   Plasmid

   #155136

   Purpose

   Expresses N-terminally GFP- and HA-tagged E. coli GlpG D243G/L244G/F245G/M247G/S248G/M249G/A250G variant from pGEX 4P-1

   Depositor

   Sinisa Urban

   Article

   Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

   Insert

   GlpG (glpG Escherichia coli)
   

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   D243G/L244G/F245G/M247G/S248G/M249G/A250G

   Promoter

   tac

   Available since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUG-T2A-H93R

   Plasmid

   #140739

   Purpose

   Lentiviral vector to overexpress the autism-associated PTEN mutation H93R and GFP as two separate proteins

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   GFP-T2A-PTENH93R (PTEN Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   H93R

   Promoter

   h

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUG-PtenN276S

   Plasmid

   #140737

   Purpose

   Lentiviral vector to overexpress the PTEN mutation N276S as a GFP-PTEN fusion protein

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   PTEN – phosphatase and tensin homolog (PTEN Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N276S

   Promoter

   hUbiC

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUG-PtenW274L

   Plasmid

   #140736

   Purpose

   Lentiviral vector to overexpress the autism-associated PTEN mutation W274L as a GFP-PTEN fusion protein

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   GFP-PTEN (PTEN Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   W274L

   Promoter

   hUbiC

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. FUG-PtenD252G

   Plasmid

   #140735

   Purpose

   Lentiviral vector to overexpress the autism-associated PTEN mutation D252G as a GFP-PTEN fusion protein

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   GFP-PTEN (PTEN Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   D252G

   Promoter

   hUbiC

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. FUG-PtenH93R

   Plasmid

   #140733

   Purpose

   Lentiviral vector to overexpress the autism-associated PTEN mutation H93R as a GFP-PTEN fusion protein

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   GFP-PTEN (PTEN Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   H93R

   Promoter

   hUbiC

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBB0238-TEV-His12

    Plasmid

    #137038

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0238 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66635.2
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Human INPP5A

    Plasmid

    #129587

    Purpose

    Expression of human INPP5A in bacteria

    Depositor

    Christophe Erneux

    Article

    Erneux et al Eur J Biochem. 1995 Dec 1;234(2):598-602

    Insert

    Type I inositol 5-phosphatase (INPP5A Human)
    

    Use

    Tags

    Maltose binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNH-TrxT-S18

    Plasmid

    #127826

    Purpose

    Production of recombinant human ribosomal protein S18

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPS18 (RPS18 Human)
    

    Use

    Tags

    His6 - Thioredoxin - TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pK_P-GAP-LM_lacO-cP-AOX1-sAR

    Plasmid

    #126745

    Purpose

    yeast plasmid overexpressing sLovA,CPR and LacI-Mit1AD

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    slovA,cpr
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    GAP

    Available since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pK_P-ICL1-LM_lacO-cP-AOX1-sAR

    Plasmid

    #126744

    Purpose

    yeast plasmid overexpressing sLovA,CPR and LacI-Mit1AD

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    slovA,cpr
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    ICL1

    Available since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPICZ-pre-Ost1-pro-alphaf-Btl2

    Plasmid

    #117665

    Purpose

    Study secretion efficiency of Btl2

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-Ost1-pro-alphaf-Btl2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    This plasmid encodes the lipase Btl2 together wit…

    Promoter

    pAOX1

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPN150

    Plasmid

    #91679

    Purpose

    Express sgRNA targeting human TSNARE1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    TSNARE1 (TSNARE1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPN151

    Plasmid

    #91680

    Purpose

    Express sgRNA targeting human TSNARE1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    TSNARE1 (TSNARE1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPN224

    Plasmid

    #91623

    Purpose

    Express sgRNA targeting human GRM3

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    GRM3 (GRM3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPN225

    Plasmid

    #91624

    Purpose

    Express sgRNA targeting human GRM3

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    GRM3 (GRM3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPN176

    Plasmid

    #91627

    Purpose

    Express sgRNA targeting human IDO2

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    IDO2 (IDO2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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