-
Plasmid#158065PurposeExpressing evolved CasTA of dCas9 fused AsiA variant 2.1DepositorInsertdCas9-AsiA_m2.1
UseTagsExpressionBacterialMutationPromoterTetOAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
EC67907
Plasmid#211794PurposeOver expresses Cas9 and GR-GRF-GIF. Must receive guide cassettesDepositorInsertHptII, Cas9, GR-GRF-GIF
UseTagsExpressionPlantMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXZ13lacZalpha
Plasmid#160229Purposeplaces LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal platesDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
PJQ49
Plasmid#134657PurposeContains Promoter 3 with bsa1 cut sites for inserting sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAnderson BBa_J23115Available sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAW016-2
Plasmid#85613PurposeCas9-tracrRNA integration vector for Bacillus subtilisDepositorInsertcas9-tracrRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
PJQ47
Plasmid#134646PurposeContains Promoter 1 with bsa1 cut sites for inserting sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAnderson BBa_J23117Available sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
PJQ48
Plasmid#134647PurposeContains Promoter 2 with bsa1 cut sites for inserting sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAnderson BBa_J23114Available sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEG302-SunTagng-22aa
Plasmid#115345PurposeEncodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genomeDepositorInsertNOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKEW397_AcrIIA15
Plasmid#138285PurposeExpression of AcrIIA15 proteinDepositorInsertAcrIIA15
UseCRISPRTagsMBPExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKEW394_AcrIIA14
Plasmid#138283PurposeExpression of AcrIIA14 proteinDepositorInsertAcrIIA14
UseCRISPRTagsSFGFPExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ146
Plasmid#69298PurposeGolden Gate recipient and Gateway entry vector; assembly of 6 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHdCas9
Plasmid#137080PurposeExpression of Himar-dCas9 in bacteriaDepositorInsertHimar-dCas9
UseTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAW014-2
Plasmid#85612PurposeMulti-gRNA delivery vector containing ugtP-gRNA.P395T for Bacillus subtilisDepositorInsertugtP-gRNA.P395T
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141B
Plasmid#69291PurposeGateway entry vector; single gRNA under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMLS134
Plasmid#73714PurposeSapTrap destination vector for building sgRNA expression vectors.DepositorInsertpU6::sgRNA
UseCRISPRTagsExpressionWormMutationSapI insertion sitePromoterC. elegans U6 snRNA pol III promoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC1825_pCR8-dCasRx-RBM38
Plasmid#118662PurposeGateway Donor; dCasRx-RBM38 ORF; No promoterDepositorInsertdCasRx-RBM38
UseCRISPRTagsExpressionMammalianMutationPromoterNo promoterAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH2971
Plasmid#100955PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR
Plasmid#68347PurposeAAV plasmid expressing the FlpO recombinase. with empty gRNADepositorInsertFlPO
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ145
Plasmid#69297PurposeGolden Gate recipient and Gateway entry vector; assembly of 5 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
EC67908
Plasmid#211795PurposeOver expresses Cas12a and GR-GRF-GIF. Must receive guide cassettesDepositorInsertHptII, Cas12a, GR-GRF-GIF
UseTagsExpressionPlantMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC1816_pCR8-gSMN2-DN4
Plasmid#118649PurposeTransient transfection; Expressed SMN2-DN4 CasRx gRNA; Gateway DonorDepositorInsertgSMN2-DN4
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP2820
Plasmid#160669PurposeMobile CRISPRi sfGFP 'test', empty sgRNADepositorInsertempty sgRNA (BsaI)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141D
Plasmid#69293PurposeGateway entry vector; single gRNA under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCD061
Plasmid#113315PurposeW108 scRNA.b1DepositorInsertW108 scRNA.b1_MS2
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFD4d
Plasmid#83954PurposeModified from pCFD4, with the vermillion marker and the attB site removedDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterdU6-1; dU6-3Available sinceOct. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ2018
Plasmid#140665PurposedCas9, LuxR & Csy4DepositorInsertdCas9, LuxR & Csy4
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTEAD1.1.0-gDNA
Plasmid#112425PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TEAD1DepositorInsertTEAD1 (TEAD1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ148
Plasmid#69300PurposeGolden Gate recipient and Gateway entry vector; assembly of 8 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJMP2822
Plasmid#160670PurposeMobile CRISPRi sfGFP 'test', empty sgRNADepositorInsertempty sgRNA (BsaI)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC1823_pCR8-RBFOX1N-dCasRx-C
Plasmid#118657PurposeGateway Donor; RBFOX1N-dCasRx-C ORF; No promoterDepositorInsertRBFOX1N-dCasRx-C
UseCRISPRTagsExpressionMammalianMutationPromoterNo promoterAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC1813_pCR8-gSMN2-DN1
Plasmid#118646PurposeTransient transfection; Expressed SMN2-DN1 CasRx gRNA; Gateway DonorDepositorInsertgSMN2-DN1
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC1814_pCR8-gSMN2-DN2
Plasmid#118647PurposeTransient transfection; Expressed SMN2-DN2 CasRx gRNA; Gateway DonorDepositorInsertgSMN2-DN2
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC1815_pCR8-gSMN2-DN3
Plasmid#118648PurposeTransient transfection; Expressed SMN2-DN3 CasRx gRNA; Gateway DonorDepositorInsertgSMN2-DN3
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCD315
Plasmid#113321PurposepBAD-W108 scRNA.b1DepositorInsertscRNA.b1_MS2
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pV1081
Plasmid#111425PurposeSolo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1DepositorInsertCaCas9/sgADE-NT2
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJMP2824
Plasmid#160671PurposeMobile CRISPRi sfGFP 'test', empty sgRNADepositorInsertempty sgRNA (BsaI)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-AsiA
Plasmid#158063PurposeExpressing CasTA of dCas9 fused AsiADepositorInsertdCas9-AsiA
UseTagsExpressionBacterialMutationPromoterTetOAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKEW215_AcrIIA13
Plasmid#138281PurposeExpression of AcrIIA13 proteinDepositorInsertAcrIIA13
UseCRISPRTagsMBPExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSABEd
Plasmid#133968PurposeExpresses ABEd CRISPR base editor and gRNA scaffold in StreptomycesDepositorArticleInsertABEd
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRPR1(1xTetO)_gRNA_handle_RPR1t
Plasmid#62966PurposeaTc inducible gRNA expression vectorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterRPR1Available sinceMarch 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ147
Plasmid#69299PurposeGolden Gate recipient and Gateway entry vector; assembly of 7 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCF525-AcrIIA5_gb36_lenti
Plasmid#138306PurposeLenti expression of AcrIIA5DepositorInsertAcrIIA5
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
HPRT2b-pGEM
Plasmid#69539PurposegRNA targeting human HPRT geneDepositorInsertHPRT gRNA2
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJMP2754
Plasmid#160666PurposeMobile CRISPRi sfGFP 'test', empty sgRNADepositorInsertempty sgRNA (BsaI)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSABEn
Plasmid#133969PurposeExpresses ABEn CRISPR base editor and gRNA scaffold in StreptomycesDepositorArticleInsertABEn
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2CT-Cdi
Plasmid#83044PurposeExpresses Corynebacterium diphtheriae Cas9 (CdiCas9)DepositorInsertCdiCas9
UseTagsHis-MBPExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCF525-AcrIIA4_gb39_lenti
Plasmid#138305PurposeLenti expression of AcrIIA4DepositorInsertAcrIIA4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRB1081
Plasmid#71479PurposeExpresses gRNA for dpy-10 conversion using NGAG PAM and VQR Cas9 variantDepositorInsertdpy-10 gRNA
UseCRISPRTagsExpressionWormMutationPromoterU6Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZBTB12.1.0-gDNA
Plasmid#113769PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB12DepositorInsertZBTB12 (ZBTB12 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTFAP2B.1.0-gDNA
Plasmid#113791PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFAP2BDepositorInsertTFAP2B (TFAP2B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only