We narrowed to 8,419 results for: BLI;

1. lenti-UCOE-dCas9-BFP-VP64

   Plasmid

   #196717

   Purpose

   CRISPR-activation. dCas9-VP64 with TagBFP for sorting.

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-TagBFP-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PGAM5

   Plasmid

   #38976

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PGAM5 (PGAM5 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pGL003

   Plasmid

   #215335

   Purpose

   amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank promoter)

   Depositor

   Gene-Wei Li

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pSpank

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-fDIO-mCherry-WPREpA

   Plasmid

   #177328

   Purpose

   Control vector for Flp-dependent DREADD constructs

   Depositor

   Ulrik Gether

   Insert

   mCherry
   

   Use

   AAV; Flp-frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human Synapsin-1

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-DO-ChroME2f-ST-P2A-GCaMP8s

   Plasmid

   #227786

   Purpose

   Cre off - Cation channelrhodopsin ChroME2f fused to GCaMP8m and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Insert

   DO-ChroME2f-ST-P2A-GCaMP8s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. USP2

   Plasmid

   #36894

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   USP2 (USP2 Human)
   

   Use

   Tags

   His tag with thrombin cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 19, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

7. BirA(R118G)-HA destination vector

   Plasmid

   #53581

   Purpose

   To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)

   Depositor

   Karl Kramer

   Type

   Empty backbone

   Use

   Tags

   BirA(R118G) and HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGs-Venus

   Plasmid

   #127346

   Purpose

   Expresses Venus constitutively via the CAGGS promoter in mammalian cells

   Depositor

   Phil Sharp

   Insert

   Venus Fluorescent Protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS Promoter

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro

   Plasmid

   #28289

   Purpose

   3rd generation lentiviral plasmid; This plasmid is the backbone for the DECIPHER libraries (currently DISCONTINUED)

   Depositor

   Gus Frangou, Alex Chenchik

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-CAG-jGCaMP8m-WPRE

    Plasmid

    #179255

    Purpose

    AAV-mediated expression of GCaMP8m under the CAG promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQCXIP-VEGFA121

    Plasmid

    #73017

    Purpose

    Retroviral expression of human VEGFA121

    Depositor

    Michael Grusch

    Insert

    VEGFA (VEGFA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-Impact-T4gp43(exo-)

    Plasmid

    #228202

    Purpose

    Expression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219A

    Depositor

    Stephen Benkovic

    Insert

    43
    

    Use

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    Mutated aspartate 219 to alanine (D219A), elimina…

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS L30 mRuby3-Rab18

    Plasmid

    #166431

    Purpose

    Rab18 fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Insert

    Rab18
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    L30 weak promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSB700-mouse-TTN-Cerulean

    Plasmid

    #128325

    Purpose

    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)

    Depositor

    Alejandro Chavez

    Insert

    gRNA against mouse TTN for activation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. ORFtag_TetR-SD_Frame2

    Plasmid

    #220982

    Purpose

    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame2

    Depositor

    Alexander Stark

    Insert

    TetR repressor
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. ORFtag_TetR-SD_Frame3

    Plasmid

    #220983

    Purpose

    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame3

    Depositor

    Alexander Stark

    Insert

    TetR repressor
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9(BB)-2A-miRFP670

    Plasmid

    #91854

    Purpose

    Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988

    Depositor

    Ralf Kuehn

    Inserts

    hSpCas9-2A-miRFP670
    BB-guide RNA
    

    Use

    CRISPR

    Tags

    2A-miRFP670 and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh and U6

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-tMCK-mem-TurboID

    Plasmid

    #149410

    Purpose

    Muscle-specific expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Insert

    mem-TurboID
    

    Use

    AAV

    Tags

    HA and V5

    Expression

    Mutation

    Promoter

    tMCK

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-3'TwStrep

    Plasmid

    #208053

    Purpose

    Empty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    Tags

    Twin Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. LentiCROP-PuroR-P2A-GFP-U6

    Plasmid

    #200029

    Purpose

    Lentiviral CROP-seq vector backbone with PuroR-P2A-GFP for cloning DNA Ticker Tapes and U6 for pegRNAs

    Depositor

    Jay Shendure

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    PuroR-P2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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