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pGBScomp Low

Plasmid

#223203

Purpose

Group B Streptococcus Low Expression Modular Complementation Vector

Depositor

Thomas Hooven

Article

Hillebrand et al Mol Microbiol. 2025 Nov;124(5):449-461.

Insert

N/A

Use

Complementation vector

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pXD80RFP-pXD68Kan2-RFP

Plasmid

#225329

Purpose

RSF1010-based broad host-range plasmid engineered from pXD68Kan2 encoding RFP, kanamycin resistance

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

RFP

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCB6-X:31HA

Plasmid

#74371

Purpose

Mammalian expression of wild-type HA from the X:31 strain of influenza virus

Depositor

Judith White

Article

Qiao et al Mol Biol Cell. 1999 Aug;10(8):2759-69.

Insert

HA

Expression

Mammalian

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
pCMV4-Tax(M20)

Plasmid

#23285

Depositor

Warner Greene

Article

Smith et al Genes Dev. 1990 Nov . 4(11):1875-85.

Insert

Tax(M20)

Expression

Mammalian

Mutation

T130A L131S

Available Since

May 3, 2010

Availability

Academic Institutions and Nonprofits only
pOL2339

Plasmid

#37839

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

Ub-V

Tags

mCherry

Expression

Worm

Promoter

nhx-2

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pJMH143

Plasmid

#186646

Purpose

aadA.co-TaadA-PJ23117-eYFP.co between homology arms for pilA

Depositor

Adam Arkin

Article

Jacob M. et al bioRxiv

Insert

aadA.co-TaadA-PJ23117-eYFP.co

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR PKAKTRn-Clover

Plasmid

#177199

Purpose

Gateway entry vector encoding PKAKTRnew-Clover

Depositor

Markus Covert

Article

Kudo et al Cell Syst. 2022 May 18;13(5):376-387.e8.

Insert

PKAKTRnew-mClover

Expression

Bacterial

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pFLxR1

Plasmid

#149496

Purpose

Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Chloramphenicol Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMAM-RecG-EGFP

Plasmid

#31274

Depositor

Andrew Pierce

Article

Killen et al BMC Mol Biol. 2012 Oct 30;13:33. doi: 10

Insert

RecG-EGFP

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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PM-SP!TB

Plasmid

#48650

Purpose

Bacterial SP crRNA expression: targets SP to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pKS2236

Plasmid

#37833

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

YFP

Tags

YFP

Expression

Worm

Promoter

nhx-2

Available Since

Jan. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pJMH058

Plasmid

#186640

Purpose

aadA.co-TaadA-PcpcB-eYFP.co between homology arms for pilA

Depositor

Adam Arkin

Article

Jacob M. et al bioRxiv

Insert

aadA.co-TaadA-PcpcB-eYFP.co

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pPEE27

Plasmid

#128367

Purpose

Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 3, a small gene-free region. Contains the nourseothricin resistance marker (NAT)

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Type

Empty backbone

Use

Unspecified

Promoter

ACT1

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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PM-TD!TB

Plasmid

#48656

Purpose

Bacterial TD crRNA expression: targets TD to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial TD crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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PM-NM!TA

Plasmid

#48651

Purpose

Bacterial NM crRNA expression: targets NM to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial NM crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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PM-TD!TA

Plasmid

#48655

Purpose

Bacterial TD crRNA expression: targets TD to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial TD crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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G1S-HA-pCB6

Plasmid

#74373

Purpose

Mammalian expression of a G1S mutant HA from the X:31 strain of influenza virus

Depositor

Judith White

Article

Qiao et al Mol Biol Cell. 1999 Aug;10(8):2759-69.

Insert

HA

Expression

Mammalian

Mutation

G1S

Promoter

CMV

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
G1V-HA-pCB6

Plasmid

#74372

Purpose

Mammalian expression of a G1V mutant HA from the X:31 strain of influenza virus

Depositor

Judith White

Article

Qiao et al Mol Biol Cell. 1999 Aug;10(8):2759-69.

Insert

HA

Expression

Mammalian

Mutation

G1V

Promoter

CMV

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
PM-NM!TB

Plasmid

#48652

Purpose

Bacterial NM crRNA expression: targets NM to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial NM crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
PM-SP!TA

Plasmid

#48649

Purpose

Bacterial SP crRNA expression: targets SP to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pKDcas9 71 cerulean

Plasmid

#230954

Purpose

aTc inducible knockdown of FliZ and CRP

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDcas9 13 cerulean

Plasmid

#230955

Purpose

aTc inducible knockdown of CRP and HNS

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDcas9 24 cerulean

Plasmid

#230956

Purpose

aTc inducible knockdown of Fis and mcaS

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDcas9 35 cerulean

Plasmid

#230957

Purpose

aTc inducible knockdown of HNS and CsgD

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDcas9 46 cerulean

Plasmid

#230958

Purpose

aTc inducible knockdown of mcaS and FlhDC

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDcas9 57 cerulean

Plasmid

#230959

Purpose

aTc inducible knockdown of CsgD and FliZ

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDcas9 72 cerulean

Plasmid

#230961

Purpose

aTc inducible knockdown of FliZ and Fis

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDcas9 14 cerulean

Plasmid

#230962

Purpose

aTc inducible knockdown of CRP and mcaS

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDcas9 47 cerulean

Plasmid

#230965

Purpose

aTc inducible knockdown of mcaS and FliZ

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDcas9 51 cerulean

Plasmid

#230966

Purpose

aTc inducible knockdown of CsgD and CRP

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge