We narrowed to 4,360 results for: 256
-
Plasmid#188524PurposeEncodes the biotin proximity labeling enzyme MicroID2 with a 2X mitochondrial targeting sequence (MTS) for expression and stable integration into mammalian cellsDepositorInsert2XMTS-MicroID2
UseTransposon mediated gene integrationTagsFLAGExpressionMammalianPromoterEF1a/RPBSAAvailable SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYT1 reporter
Plasmid#197275PurposeEncodes for viral expression of wild type Synaptotagmin 1 (SYT1) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).DepositorInsertSYT1 and HaloTag (Syt1 Rat)
UseLentiviralTagsFLAGMutationN/APromoterhSyn (human synapsin I promoter)Available SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEND-446_Cacnes_ProA
Plasmid#225609PurposeSuicide C. acnes vector to generate ProA (PPA_RS04250) deletion leaving an Ery resistance cassette flanked by BxbI recombination sitesDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKanCMV-mRuby3-18aa-Tubulin
Plasmid#74256PurposeMammalian expression of N-terminal fusion of mRuby3 to TubulinDepositorInsertTubulin
TagsmRuby3ExpressionMammalianPromoterCAG and CMVAvailable SinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.4t
Plasmid#210256PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertsmCherry
superfolder GFP
TagsssraExpressionBacterialPromoteripaH7.8p and rpsMAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYB2 reporter
Plasmid#197276PurposeEncodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).DepositorInsertSYB2 and HaloTag (Vamp2 Rat)
UseLentiviralTagsFLAGMutationN/APromoterhSyn (human synapsin I promoter)Available SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-evoCjCas9-ABE8e_gRNA
Plasmid#202561PurposehSyn-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)DepositorInsertevoCjCas9-ABE8e
UseAAVAvailable SinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEND-418_Cacnes_LysA
Plasmid#225607PurposeSuicide C. acnes vector to generate LysA (PPA_RS06345) deletion leaving an Ery resistance cassette flanked by BxbI recombination sitesDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSBbi_RP_MicroID2-FLAG-NES
Plasmid#188525PurposeEncodes the biotin proximity labeling enzyme MicroID2 with a nuclear export sequence (NES) for expression and stable integration into mammalian cellsDepositorInsertMicroID2
UseTransposon mediated gene integrationTagsFLAGExpressionMammalianPromoterEF1a/RPBSAAvailable SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGLACTE-HT51-13
Plasmid#99256PurposeContains normal CGG repeats (29 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. To generate capillary electrophoresis size standards.DepositorInsertFMR1 (FMR1 Human)
TagseGFPAvailable SinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-HiFi-nCas9n-A72
Plasmid#125646Purposemicro-injection of poly-adenylated HiFi-nCas9n RNADepositorInsertHiFi-nCas9n
UseRna in vitro transcription vectorMutationR691A from wild-type Cas9PromoterT3Available SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-ODC1
Plasmid#133256PurposeExpress FLAG-tagged ODC1 in mammalian cellsDepositorInsertODC1 (ODC1 Budding Yeast)
UseRetroviralTags3xFLAGExpressionMammalianMutationcodon-optimized for expression in human cellsAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRV DYRK2
Plasmid#12568DepositorAvailable SinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
PNXGW
Plasmid#48256PurposeSplit-GFP assay in plantsDepositorTypeEmpty backboneUseBinary gateway vectorTags6xHIS and YFP aa1-172Promoter35SAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT-CuSeCat
Plasmid#42568DepositorInsertmutated calmodulin gene
TagsPolyhistidine (6×His) region, TEV recognition sit…ExpressionBacterialMutationchanged Phenylalanine 92 to Glutamine acid, Valin…PromoterT7Available SinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NCK2-3/3
Plasmid#91256PurposeProtein expression and purification of human SH3 domain construct NCK2-3/3DepositorInsertNCK2-3/3 (NCK2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GcACR_457-EYFP
Plasmid#103137PurposeAnion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cellsDepositorInsertAnion channelrhodopsin GcACR_457
TagsEYFPExpressionMammalianMutationhuman codon optimizedPromoterCMVAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-133
Plasmid#92256PurposePlasmid for expression of NHA-tagged human TNRC6C CED in mammalian cellsDepositorInsertTNRC6C CED (TNRC6C Human)
TagsNHAExpressionMammalianMutation.C-terminal effector domain (CED): aa 1369-1690PromoterCMVAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
mycUb-25aa
Plasmid#20256DepositorInsertUbiquitin-25 amino acid extension (RBX1 Human)
TagsmycExpressionMammalianMutationUbiquitin fused to N-terminus of GFP. Glycine 76 …Available SinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
1010Av2
Plasmid#125642PurposeExpresses FAR protein under 10xUAS controlDepositorInsertFAR protein
ExpressionInsectPromoter10xUASAvailable SinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
1010Bv2
Plasmid#125643PurposeExpresses FAR protein under 10xUAS controlDepositorInsertFAR protein
ExpressionInsectPromoter10xUASAvailable SinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-443_Cacnes_HisB
Plasmid#225608PurposeSuicide C. acnes vector to generate HisB (PPA_RS05815) deletion leaving an Ery resistance cassette flanked by BxbI recombination sitesDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL CusS NarX Chimera
Plasmid#225652PurposeExpresses a NarX-based chimera capable of sensing copper (II). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertCusS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available SinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NrsS NarX Chimera
Plasmid#225653PurposeExpresses a NarX-based chimera capable of sensing nickel (II). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertNrsS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL RssA NarX Chimera
Plasmid#225654PurposeExpresses a NarX-based chimera capable of sensing iron (III). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertRssA-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL VanS NarX Chimera
Plasmid#225655PurposeExpresses a NarX-based chimera capable of sensing vancomycin. For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertVanS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
PtetA-PLacO3O1 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225645PurposetetA and LacO3O1 promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInserttetR/tetA and LacO3O1 in tandem conformation
TagsmCherryExpressionBacterialPromotertetA and LacO3O1 promotersAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
PLacO3O1-PtetA 250 bp with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225648PurposeLacO3O1 and tetA promoters in tandem conformation with 250bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInsertLacO3O1 and tetR/tetA in tandem conformation
TagsmCherryExpressionBacterialPromoterLacO3O1 and tetA promotersAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PLacO3O1-PtetA 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225647PurposeLacO3O1 and tetA promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInsertLacO3O1 and tetR/tetA in tandem conformation
TagsmCherryExpressionBacterialPromoterLacO3O1 and tetA promotersAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PtetA-PLacO3O1 350 bp with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225646PurposetetA and LacO3O1 promoters in tandem conformation with 350bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInserttetR/tetA and LacO3O1 in tandem conformation
TagsmCherryExpressionBacterialPromotertetA and LacO3O1 promotersAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAPM-miR30-IFI16-ts1
Plasmid#174256PurposeIFI16 knockdownDepositorAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-SOD1
Plasmid#222568PurposePiggyBac transposon plasmid for doxycycline inducible expression of SOD1DepositorInsertSOD1 (SOD1 Human)
ExpressionMammalianAvailable SinceAug. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4405
Plasmid#215256PurposeModule for the expression of nptII, the N. nambi CPH, LUZ and H3H gene flankeds by insulators on both sidesDepositorInsertSF-MAR10-SF-nptII-CPH-LUZ-H3H-MAR10-SF
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH4508
Plasmid#200256PurposePflp-18 LoxP EBFP LoxP TeTx mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of TeTx RFPDepositorInsertTeTx
TagsmCherryExpressionWormPromoterPflp-18Available SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC129
Plasmid#202256PurposeLevel 2 for strain-specific barcode DNA tag with bc156 through Tn7 bacterial insertion, Kanamycin selectable marker and tagRFP fluorescent markerDepositorInsertbc156
ExpressionBacterialAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P123G wgMDH
Plasmid#204256PurposeBacterial expression of P123G wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationP123GAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYT1deltaC2AB reporter
Plasmid#197279PurposeEncodes for viral expression of Synaptotagmin 1 (SYT1) (1 - 140 a.a.) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).DepositorInsertSYT1 (1 - 420 a.a.) and HaloTag (Syt1 Rat)
UseLentiviralTagsFLAGPromoterhSyn (human synapsin I promoter)Available SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pm_MSP4-Cd4-bio-His
Plasmid#126820PurposeExpression of recombinant P. malariae protein in mammalian cellsDepositorInsertMSP4 (PmUG01_04025600 Plasmodium malariae)
TagsratCd4(d3+4)-bio-6HisExpressionMammalianPromoterCMVAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GcACR_145-EYFP
Plasmid#103138PurposeAnion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cellsDepositorInsertAnion channelrhodopsin GcACR_145
TagsEYFPExpressionMammalianMutationhuman codon optimizedPromoterCMVAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GcACR_439-EYFP
Plasmid#103142PurposeAnion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cellsDepositorInsertAnion channelrhodopsin GcACR_439
TagsEYFPExpressionMammalianMutationhuman codon optimizedPromoterCMVAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only