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ApoE3_47-300_mCh-SspB (pBS1070)

Plasmid

#185293

Purpose

For the mammalian expression of the human protein ApoE3_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3_47-300

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE4_47-300_mCh-SspB (pBS1069)

Plasmid

#185292

Purpose

For the mammalian expression of the human protein ApoE4_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE4_47-300

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE2_47-300_mCh-SspB (pBS1068)

Plasmid

#185291

Purpose

For the mammalian expression of the human protein ApoE2_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE2_47-300

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTRETightBI-RY-0

Plasmid

#31463

Depositor

Phil Sharp

Article

Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

Insert

eYFP and mCherry

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2012

Availability

Academic Institutions and Nonprofits only

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multi-shRNA vector

Plasmid

#12391

Depositor

Zuoshang Xu

Article

Xia et al Biotechniques. 2006 Jul . 41(1):64-8.

Insert

mSOD2 shRNA (Sod2 Mouse)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pLV.MRE11i

Plasmid

#15565

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2006 Mar . 80(5):2445-52.

Insert

MRE11 shRNA (MRE11 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2007

Availability

Academic Institutions and Nonprofits only
pHR_Ef1a-->flagLaM4_Notch_tTA

Plasmid

#162237

Purpose

Lentiviral vector for the expression of LaM 4 mCherry nanobody notch tTA: flagLaM4_Notch_tTA in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaM4 mCherry nanobody Notch tTA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pSUPER.MRE11i

Plasmid

#15566

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2006 Mar . 80(5):2445-52.

Insert

MRE11 shRNA (MRE11 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2007

Availability

Academic Institutions and Nonprofits only
pFA6a LEU2

Plasmid

#61923

Purpose

Expression of K. lactis LEU2 cloned into pFA6a

Depositor

David Tollervey, Jon Houseley

Article

Houseley et al Nucleic Acids Res. 2011 Nov 1;39(20):877

Insert

LEU2

Use

Yeast genomic targeting

Tags

Expression

Mutation

Promoter

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a URA3

Plasmid

#61924

Purpose

Expression of S. cerevisiae URA3 cloned into pFA6a

Depositor

David Tollervey, Jon Houseley

Article

Houseley et al Nucleic Acids Res. 2011 Nov 1;39(20):877

Insert

URA3

Use

Yeast genomic targeting

Tags

Expression

Mutation

Promoter

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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HA-p73α-pcDNA3

Plasmid

#22102

Depositor

William Kaelin

Article

Jost et al Nature. 1997 Sep 11. 389(6647):191-4.

Insert

tumor protein p73 (TP73 Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Promoter

Available since

Sept. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->GssmycLaG16LaG2_P2A_NLSIFP

Plasmid

#162228

Purpose

Lentiviral vector for the expression of Myc GFP nanbody LaG16LaG2 with IFP NLS in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaG16LaG2 and IFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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JC Mad-1

Plasmid

#25626

Depositor

Peter Howley

Article

Howley et al J Virol. 1980 Dec . 36(3):878-82.

Insert

JC Virus Mad-1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
pHR_EF1a-->HA-mtLaG2

Plasmid

#162225

Purpose

Lentiviral vector for the expression of HA GFP nanobody Lag2 in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

anti-GFP nanobody LaG2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3GS-->sGFP-P2A-BFP

Plasmid

#162234

Purpose

Lentiviral vector for the expression of TRE3GS-->sGFP-P2A-BFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

EGFP and BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_TRE3GS-->sGFP-P2A-mCherry

Plasmid

#162232

Purpose

Lentiviral vector for the expression of TRE3GS-->sGFP-P2A-mCherry in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

EGFP and mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-miR30

Plasmid

#20670

Depositor

Bryan Cullen

Article

Zeng et al Mol Cell. 2002 Jun . 9(6):1327-33.

Insert

miR-30 (MIR30A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pHR_TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP

Plasmid

#162233

Purpose

Lentiviral vector for the expression of GFP nanobody LaG16LaG2 -IFP : TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

anti-GFP nanobody lag16lag2 and IFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->myc-mtC11L34

Plasmid

#162226

Purpose

Lentiviral vector for the expression of Myc mtC11L34 in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

anti-PNE scFv C11L34

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->HA-mtC11L34

Plasmid

#162227

Purpose

Lentiviral vector for the expression of HA mtC11L34 in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

mtC11L34

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge