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pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-NEDD1-gTBD
Plasmid#196878PurposeNeuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.DepositorInsertmOrange2-N-gTBD
UseCre/LoxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-C-6His-mRplp1
Plasmid#173153Purposemouse Ribosomal Protein Lateral Stalk Subunit P1 (Rplp1) expression plasmid in mammalian cellsDepositorInsertORF clone Ribosomal Protein Lateral Stalk Subunit P1 (Rplp1) (Rplp1 Mouse)
UseTagsHisExpressionMammalianMutationWild typePromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBactin-PACT-mOrange2-hCM1
Plasmid#196868PurposeExpression of Pericentrin-AKAP450 centrosomal targeting (PACT) domain fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). PACT targets CM1 to the centrosomeDepositorInsertPACT-mOrange2-hCM1 (Akap9 Rat)
UseTagsExpressionMammalianMutationPromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Nluc
Plasmid#180501PurposeMammalian expression of NlucDepositorInsertNluc
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CD63-L7Ae
Plasmid#180503PurposeHuman CD63-L7Ae fusion proteinDepositorInsertHuman CD63-L7Ae fusion protein
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-Nluc-2xC/D box
Plasmid#180504PurposeMammalian expression of Nluc-2xC/D boxDepositorInsertNluc-2xC/D box
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TEVp
Plasmid#162599PurposeTEV protease under CMV promoterDepositorInsertTEV protease
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Nluc(W12stop)
Plasmid#180502PurposeMammalian expression of Nluc(W12stop)DepositorInsertNluc(W12stop)
UseTagsFLAGExpressionMammalianMutationW12(UGG) to amber stop codon(UAG)PromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCBASceI
Plasmid#26477PurposeI-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI siteDepositorInsertpCBASceI
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-NlucP
Plasmid#162595PurposeC-terminally PEST-tagged NanoLuc under TRE3G promoterDepositorInsertNanoLuc-PEST
UseTagsPESTExpressionMammalianMutationPromoterTRE3G promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-EGFP(W58stop)-HiBiT
Plasmid#180508PurposeMammalian expression of EGFP(W58stop)-HiBiTDepositorInsertEGFP(W58stop)
UseTagsHiBiTExpressionMammalianMutationW58(UGG) to amber stop codon(UAG)PromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
PasCas13b-gRNA-EGFP(W58stop)
Plasmid#180507PurposePasCas13b-gRNA targeting EGFP(W58stop) for RNA editingDepositorInsertPasCas13b-gRNA targeting EGFP(W58stop)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
PasCas13b-gRNA-Nluc(W12stop)
Plasmid#180506PurposePasCas13b-gRNA targeting Nluc(W12stop) for RNA editingDepositorInsertPasCas13b-gRNA targeting Nluc(W12stop)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBA310
Plasmid#190002PurposeInducible expression vector for Trypanosoma bruceiDepositorTypeEmpty backboneUseExpression vector for trypanosoma bruceiTagsExpressionMutationPromoterAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-Nluc(W12stop)-2xC/D box
Plasmid#180505PurposeMammalian expression of Nluc(W12stop)-2xC/D boxDepositorInsertNluc(W12stop)-2xC/D box
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-LoxP-mKate-LoxP-EGFP
Plasmid#167940PurposeCMV promoter-LoxP-mKate-LoxP-EGFPDepositorInsertLoxP-mKate-LoxP-EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNK083b
Plasmid#156366PurposeExpresses heterlogous ribosomal proteinsDepositorInsertS16 ribosomal gene
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP(HiBiT)
Plasmid#162589PurposeN-terminally HiBiT-tagged EGFP under CAG promoterDepositorInsertEGFP
UseTagsHiBiTExpressionMammalianMutationPromoterCAG promoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
T7-phiC31-nos3'UTR
Plasmid#182192Purposein vitro transcription of phiC31 integrase mRNADepositorInsertphiC31 integrase
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only
