Addgene

Showing: 301 - 320 of 1667 results

Results per page:

Sort results by:

pCMV-RA-HRas-IRES2-Lyn-iRFP682

Plasmid

#180584

Purpose

RA-Hras and Lyn-iRFP682

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

RA-Hras and Lyn-iRFP682

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA-FRT-FAK0

Plasmid

#45047

Purpose

FAK fret sensor without helix

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pTol-Promoter:NLS-tdMCP-eGFP

Plasmid

#86248

Purpose

For generating transgenic lines that ubiquitously expressing the fluorescent MCP reporter

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Insert

beta Actin promoter:NLS-tdMCPeGFP

Use

Used with transposase to make transient or stable…

Tags

NLS-tdMCP-eGFP

Expression

Mutation

Promoter

Beta Actin

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pOGG004

Plasmid

#113979

Purpose

Vector construction module. Level 1 golden gate cloning site module with LacZ reporter

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Level 1 golden gate cloning site

Use

Tags

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTub-alpha1-Lifeact-GFP

Plasmid

#175437

Purpose

F-actin structures labelling

Depositor

Frank Bradke, Sebastián Dupraz

Article

Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

Insert

Lifeact-EGFP

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

pTub-alpha1

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA-FRT-FAK20

Plasmid

#59120

Purpose

FAK fret sensor with 20 nm helix

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDR FLIP43 GPS1-NR

Plasmid

#101085

Purpose

GA biosensor expression plasmid

Depositor

Wolf Frommer, Alexander Jones

Article

Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

Insert

GPS1-NR

Use

Tags

6HIS and cMyc

Expression

Yeast

Mutation

AtGAI E51K E54R, AtGID1C S114A F115A

Promoter

pPMA1

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pF3BGX

Plasmid

#138387

Purpose

Empty RMCE vector (phiC31 attB/loxP)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

P5' FRT3 3xP3-Venus φC31-attB loxP FRT3 P3'

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
orthodenticle

Plasmid

#37285

Depositor

Norbert Perrimon

Article

Finkelstein et al Genes Dev. 1990 Sep;4(9):1516-27.

Insert

orthodenticle (oc Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pF3BGX-mScarlet-I

Plasmid

#138391

Purpose

RMCE vector for generating C-terminal mScarlet-I fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

mScarlet-I

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pWalium-ChtVis-Tomato

Plasmid

#67756

Purpose

Expresses a fluorescent chitin reporter under Gal4 control in Drosophila.

Depositor

Paul Adler

Article

Sobala et al Development. 2015 Nov 15;142(22):3974-81

Insert

dyl - chitin binding domain - tdTomato - dyl

Use

Tags

td-tomato

Expression

Insect

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
hygII-UT-ABAleon2.15

Plasmid

#106983

Purpose

ABAleon2.15 in plant expression vector (Hygromycin resistance)

Depositor

Julian Schroeder

Article

Waadt et al Elife. 2014 Apr 15;3:e01739. doi: 10.755

Insert

ABAleon2.1

Use

Tags

Expression

Plant

Mutation

Promoter

UBQ10

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBCC155

Plasmid

#74995

Purpose

CFP-based BiFC vector for transient expression of CC (155-238) in plants

Depositor

Masamitsu Wada, Yutaka Kodama

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Type

Empty backbone

Use

Reporter plasmid

Tags

CFP (155–239)/CC155

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBCN155

Plasmid

#74994

Purpose

CFP-based BiFC vector for transient expression of CN (1-155) in plants

Depositor

Masamitsu Wada, Yutaka Kodama

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Type

Empty backbone

Use

Reporter plasmid

Tags

CFP (1–155)/CN155

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pOGG009

Plasmid

#113983

Purpose

Vector construction module. Gentamicin resistance module for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Gentamicin

Use

Tags

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDB1751

Plasmid

#174013

Purpose

pDUAL plasmid expressing GFP from 41nmt1 promoter

Depositor

Li-Lin Du

Article

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

GFP

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

41nmt1 and 41nmt1 promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-P70a-Top-T~P70a-Bottom-T

Plasmid

#87314

Purpose

Expresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

P70a

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pF3BGX-T2A-GAL4

Plasmid

#138392

Purpose

RMCE vector for generating C-terminal T2A-GAL4 fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-GAL4

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pM370

Plasmid

#173178

Purpose

(Empty Backbone) gRNA-12xMBS

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

gRNA-12xMBS

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p16 GPS1

Plasmid

#101083

Purpose

GA biosensor expression plasmid

Depositor

Wolf Frommer, Alexander Jones

Article

Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

Insert

GPS1

Use

Tags

cMyc

Expression

Plant

Mutation

Promoter

p16 (AT3G60245)

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge