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Plasmid#194802PurposecCadC-GCN4* under the control of PtetDepositorInsertcCadC-GCN4*
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBL090J
Plasmid#195045PurposecCadC-CCB3.5 under the control of PBADDepositorInsertcCadC-CCB3.5
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Leu-eΔK-Flv
Plasmid#83397Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Arg-eΔK-Flv
Plasmid#83396Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Phe-eΔK-Flv
Plasmid#83395Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-3xrGBD
Plasmid#176108PurposeVisualization of active RhoDepositorInsertrhotekin (Rtkn Mouse)
UseTagsExpressionMammalianMutationPromoterCMVdelAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lck-mTurquoise2-iLID
Plasmid#176125PurposePlasma membrane localized iLIDDepositorInsertiLID
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-mCherry-FKBP-TIAM1(DHPH)
Plasmid#176138PurposeHeterodimerization with FRB (rapamycin), Rac1 activationDepositorInsertTIAM1 (TIAM1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
P2061
Plasmid#186299PurposeExpresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoterDepositorInsertsLexA-HBD-B112
Cas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterial and YeastMutationPromoter2xLexop-minimalCyc1 and PACT1Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSB700-mouse-ACTC1-Puro
Plasmid#128326PurposeSP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)DepositorInsertgRNA against mouse ACTC1 for activation (Actc1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1/opto-a1AR-EYFP
Plasmid#20947PurposeMammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signalingDepositorInsertopto-a1AR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/opto-b2AR-EYFP
Plasmid#20948PurposeMammalian expression of opto-b2AR fused to EYFP for optical control of GPCR signalingDepositorInsertopto-b2AR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHH02_15UP436
Plasmid#120893PurposeSelection of ECF for controller implementationDepositorInsertPecf15_up436 (-60 to +20)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-frt-DIO-hM3D(Gq)-mCherry
Plasmid#217397PurposeExpresses the hM3D(Gq) excitatory DREADD fused to mCherry, in a Cre-ON and FLP-OFF dependent manner, under the control of hSyn promoterDepositorInserthM3D(Gq)-mCherry
UseAAVTagsmCherryExpressionMammalianMutationPromoterhuman Synapsin 1Available sinceMay 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-frt-DIO-hM4D(Gi)-mCherry
Plasmid#217398PurposeExpresses the hM4D(Gi) inhibitory DREADD fused to mCherry, in a Cre-ON and FLP-OFF dependent manner, under the control of hSyn promoterDepositorInserthM4D(Gi)-mCherry
UseAAVTagsmCherryExpressionMammalianMutationPromoterhuman Synapsin 1Available sinceMay 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH01_15
Plasmid#120890PurposeSelection of ECF for controller implementationDepositorInsertECF15_436
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH01_22
Plasmid#120891PurposeSelection of ECF for controller implementationDepositorInsertECF22_4450
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH01_32
Plasmid#120892PurposeSelection of ECF for controller implementationDepositorInsertECF32_1122
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH02_22UP1147
Plasmid#120894PurposeSelection of ECF for controller implementationDepositorInsertPecf22_up1147 (-60 to +20)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only