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pFunnyfarm

Plasmid

#24755

Depositor

Christopher Buck

Publication

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

CAT-ccdB cassette

Use

Gateway-adapted entry vector

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pLX301-U2AF1-WT-FLAG

Plasmid

#256552

Purpose

Lentiviral vector for expression of FLAG-tagged U2AF1 in mammalian cells

Depositor

Matthew Meyerson

Publication

Walter et al Nat Genet. 2026 Jul 1. doi: 10.1038/s415

Insert

U2AF1 (U2AF1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available since

May 26, 2026

Availability

Academic Institutions and Nonprofits only

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5'-BirA-FLAG GFP minimal CMV pCDNA5

Plasmid

#212087

Purpose

LR vector for integration of GFP into N2a FRT rtTA3 expression cells (BioID vector)

Depositor

Benjamin Blencowe

Publication

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

GFP

Expression

Mammalian

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pLX301-U2AF1-S34F-FLAG

Plasmid

#256553

Purpose

Lentiviral vector for expression of FLAG-tagged U2AF1 S34F in mammalian cells

Depositor

Matthew Meyerson

Publication

Walter et al Nat Genet. 2026 Jul 1. doi: 10.1038/s415

Insert

U2AF1 (U2AF1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

S34F

Available since

May 26, 2026

Availability

Academic Institutions and Nonprofits only

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CAG-xFNLS-T2A-EGFP-ires-puro

Plasmid

#121171

Purpose

CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.

Depositor

Timo Otonkoski

Insert

xFNLS-T2A-EGFP-ires-puro

Use

CRISPR

Tags

T2A-EGFP-ires-puro

Expression

Mammalian

Available since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Rat SARE-Arc Min Promoter-Flag Arc-Arc Genomic 3'UTR

Plasmid

#233056

Purpose

To express Flag-tagged Rat Arc from a Rat SARE-Arc minimal promoter. The Arc gene contains the Rat Arc native Genomic 3'UTR containing two introns

Depositor

Jonathan Ploski

Insert

Rat Arc (Arc Rat)

Use

AAV

Tags

Flag

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

Plasmid

#121175

Purpose

Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines

Depositor

Timo Otonkoski

Insert

GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro

Use

CRISPR

Expression

Mammalian

Available since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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HOXC13(human) GSTfusion-pET

Plasmid

#8680

Depositor

Corey Largman

Publication

Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

Insert

HOXC13 (HOXC13 Human)

Tags

GST

Expression

Bacterial

Mutation

makes protein by Coomassie blue staining

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pLenti-CMV-eGFP-hTau AP- WPRE

Plasmid

#233044

Purpose

To Express a GFP human Tau AP fusion protein from a CMV promoter

Depositor

Jonathan Ploski

Insert

eGFP-hTau AP fusion (MAPT Synthetic, Human)

Use

Lentiviral

Tags

EGFP

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3 T7 Jip1(delta JBD)

Plasmid

#51767

Purpose

" JIP-1 may suppress signal transduction by the JNK pathway"

Depositor

Roger Davis

Publication

Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

Insert

Jnk interacting protein 1 (Mapk8ip1 Mouse)

Tags

T7

Expression

Mammalian

Mutation

deleted 126-263

Available since

March 14, 2014

Availability

Academic Institutions and Nonprofits only
pEMS1497

Plasmid

#29245

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple26 (CCL27 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available since

Nov. 7, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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MigR1-Flag-TRIB3

Plasmid

#131159

Purpose

Retroviral expression of flag-tagged mouse TRIB3

Depositor

Karen Keeshan, Warren Pear

Publication

Dedhia et al Blood. 2010 Aug 26;116(8):1321-8. doi: 1

Insert

Trib3 (Trib3 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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5'-3xFLAG Kdm1a-exon minimal CMV pCDNA5

Plasmid

#212054

Purpose

LR vector for integration of Kdm1a-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Publication

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Kdm1a-exon (Kdm1a Mouse)

Expression

Mammalian

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCEFL GAL4dbd-KLF4 1-329

Plasmid

#140146

Purpose

GAL4-DNA binding domain fusion protein with the 329 N-terminal amino acids of human KLF4

Depositor

Ramiro Iglesias-Bartolome

Publication

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

KLF4 Kruppel factor (KLF4 Human)

Tags

GAL4dbd

Expression

Mammalian

Mutation

Deleted amino acids 330 to 479

Promoter

EF1a

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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ePB-Puro_TT-hYAP-5SA-P2A-tdTomato-CAAX

Plasmid

#252251

Purpose

A PiggyBac donor vector for Dox-inducible expression of YAP-5SA and membrane-localized tdTomato.

Depositor

Jun Wu

Publication

Oura et al Cell Stem Cell. 2025 Oct 2;32(10):1509-1

Insert

YAP-5SA (YAP1 Human)

Use

Piggybac transposon donor vector

Tags

P2A-tdTomato-CAAX

Expression

Mammalian

Promoter

tight TRE

Available since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Med24-exon minimal CMV pCDNA5

Plasmid

#212060

Purpose

LR vector for integration of Med24-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Publication

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Med24-exon (Med24 Mouse)

Expression

Mammalian

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmRRP41_AA

Plasmid

#148222

Purpose

Insect Expression of DmRRP41

Depositor

Elisa Izaurralde

Insert

DmRRP41 (Ski6 Fly)

Expression

Insect

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRRP41_Z

Plasmid

#148132

Purpose

Mammalian Expression of HsRRP41

Depositor

Elisa Izaurralde

Insert

HsRRP41 (EXOSC4 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1/mCherry-DMDEx23

Plasmid

#211367

Purpose

Expresses a DMD exon 23 skipping reporter in mammalian cells (mCherry-DMDEx23)

Depositor

Ulrich Lächelt

Publication

Lessl et al Bioconjug Chem. 2023 Dec 20;34(12):2263-

Insert

mCherry-DMDEx23

Tags

eGFP

Expression

Mammalian

Mutation

mCherry interrupted by mdx dystrophin exon 23 bet…

Promoter

CMV

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBrain-GFP-TACC3KDP(L566,567A)-shTACC3

Plasmid

#69117

Purpose

Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A and L567A substitutions) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

Depositor

Stephen Royle

Publication

Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

Inserts

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
shTACC3

Use

RNAi

Tags

EGFP

Expression

Mammalian

Mutation

LL (566-567aa) mutated to AA. Silent mutations i…

Promoter

CMV

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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