We narrowed to 3,163 results for: tef

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YIplac128-TEF2p-LDH-CYC1t

Plasmid

#216120

Purpose

Yeast integrative vector for constitutive expression of LDH at the LEU2 locus

Depositor

Yvonne Nygård

Publication

Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

Insert

Lactate dehydrogenase (LDH Bovine)

Expression

Yeast

Promoter

TEF2 promoter

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pPpT4- TEF2prom-TIR1-FLAG

Plasmid

#189726

Purpose

Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

Depositor

Anita Emmerstorfer-Augustin

Publication

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

TEF2

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pL2-1 ConjEpi PtEF2bsd Pt49202HMGRVenusThrFLAG

Plasmid

#139963

Purpose

Over-expression of native 3-hydroxy-3-methyl glutaryl CoA reductase

Depositor

Raffaela Abbriano

Publication

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

ConjEpi PtEF2_bsd_PttEF2 Pt49202_HMGRVenusThrFLAG_Ptt49202

Use

Synthetic Biology

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pL2-1 ConjEpi PtEF2bsd Pt49202tHMGRVenusThrFLAG

Plasmid

#139964

Purpose

Over-expression of N-terminal truncated version of native 3-hydroxy-3-methyl glutaryl CoA reductase

Depositor

Raffaela Abbriano

Publication

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

ConjEpi PtEF2_bsd_PttEF2 Pt49202_tHMGRVenusThrFLAG_Ptt49202

Use

Synthetic Biology

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-3P-ECM33_intron

Plasmid

#127601

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3

Depositor

David Bartel

Publication

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins inserted near 3′ end of intron

Expression

Yeast

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-5M-ECM33_intron

Plasmid

#127600

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3

Depositor

David Bartel

Publication

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron

Expression

Yeast

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-5P-ECM33_intron

Plasmid

#127599

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3

Depositor

David Bartel

Publication

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins inserted near 5′ end of intron

Expression

Yeast

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-ECM33_intron

Plasmid

#127598

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, ECM33 intron inserted into URA3

Depositor

David Bartel

Publication

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron

Expression

Yeast

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDSM-bc-Psmtef1-CAD-Tyhi9

Plasmid

#127730

Purpose

Yeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.

Depositor

Christopher Voigt

Publication

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Expression

Yeast

Promoter

Psmtef1

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Ptef1-ACO-Tyol036w

Plasmid

#127733

Purpose

Yeast pathway position 4. ACO transcription unit with the TEF1 promoter and YOL036w terminator.

Depositor

Christopher Voigt

Publication

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Expression

Yeast

Promoter

Ptef1

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Psptdh3-ACO-Tagtef1

Plasmid

#127734

Purpose

Yeast pathway position 4. ACO transcription unit with the S. paradoxus TDH3 promoter and A. gossypii TEF1 terminator.

Depositor

Christopher Voigt

Publication

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Expression

Yeast

Promoter

Psptdh3

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Ptef1-CSC-Tpdc1

Plasmid

#127739

Purpose

Yeast pathway position 6. CSC transcription unit with the TEF1 promoter and PDC1 terminator.

Depositor

Christopher Voigt

Publication

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Expression

Yeast

Promoter

Pfba1

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Psbtdh3-CSC-Tefm1

Plasmid

#127742

Purpose

Yeast pathway position 6. CSC transcription unit with the S. bayanus TDH3 promoter and EFM1 terminator.

Depositor

Christopher Voigt

Publication

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Expression

Yeast

Promoter

Psbtdh3

Availability

Academic Institutions and Nonprofits only

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pTPGI_dCas9_VP64

Plasmid

#49013

Purpose

encodes yeast-optimized dCas9_VP64 synthetic transcription factor

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

dCas9_VP64 (codon-optimized for expression in S. cerevisiae)

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Yeast

Promoter

pTPGI (galactose+aTc inducible)

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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p6352 MSCV-CMV-CMV-Flag-HA-JMJD6

Plasmid

#31358

Depositor

Peter Howley

Publication

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

JMJD6 (JMJD6 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available since

Oct. 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pcDNA3.1-N-Myc_WT USP7

Plasmid

#131242

Purpose

Mammalian expression of N-terminally Myc-tagged USP7

Depositor

Roger Woodgate

Publication

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV enhancer + CMV promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3 CDK8 HA (P#634)

Plasmid

#14649

Depositor

Matija Peterlin

Publication

Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cdk8 (CDK8 Human)

Tags

HA

Expression

Mammalian

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGG431

Plasmid

#165615

Purpose

Vector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)

Depositor

Marcus Noyes

Publication

Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

Insert

TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

Corrected homology relative to wild type SpCas9 (…

Promoter

TEF1

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-Cry2-mCherry-TEAD4-IDR

Plasmid

#166449

Purpose

Expresses fusion of CRY2PHR with mCherry and TEAD4-IDR

Depositor

Shuguo Sun

Publication

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

Cry2, mCherry, TEAD4-IDR(89-230) (TEAD4, CRY2 Human)

Use

Lentiviral

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pQFlag-USP7 CS puroR

Plasmid

#46752

Purpose

Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP7

Depositor

Goedele Maertens, Gordon Peters

Publication

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

C223S--catalytically inactive

Promoter

CMV

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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