We narrowed to 9,393 results for: Coli

1. pXD70Tet(HcdR)-HcdR

   Plasmid

   #191648

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (TetR), hcdR complementation

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   HcdR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pXD70Tet(CadR)-CadR

   Plasmid

   #191649

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (TetR), cadR complementation

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   CadR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. T7-10-QUAS-GFP_ColE1

   Plasmid

   #171657

   Purpose

   QUAS ten base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   T7-10-QUAS
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. T7-15-QUAS-GFP_ColE1

   Plasmid

   #171658

   Purpose

   QUAS fifteen base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   T7-15-QUAS
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. T7-TetO-QF_T7-LacO-TetR_p15A

   Plasmid

   #171659

   Purpose

   Expresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. Contains a p15A origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   T7-LacO-TetR-T7 Stop
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. T7-0-QUAS-GFP_ColE1

   Plasmid

   #171650

   Purpose

   QUAS directly downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   T7-0-QUAS
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. QUAS-0-T7-CcdB_ColE1

   Plasmid

   #171652

   Purpose

   QUAS directly upstream of a T7 promoter. Expresses CcdB in BL21(DE3) E. coli. Contains ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   CcdB-ssrA
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. T7-5-QUAS-GFP_ColE1

   Plasmid

   #171656

   Purpose

   QUAS five base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   T7-5-QUAS
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PET28a-cpLumiLuc-amylase sensor

   Plasmid

   #186293

   Purpose

   Expression alpha-amylase sensor in e coli

   Depositor

   Kirill Alexandrov

   Article

   Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

   Insert

   cpLumiLuc-amylase sensor
   

   Use

   Tags

   HisTag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PET28a-cpLumiLuc-HSA sensor

    Plasmid

    #186294

    Purpose

    Expression Human serum albumin sensor in e coli

    Depositor

    Kirill Alexandrov

    Article

    Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

    Insert

    cpLumiLuc-HSA sensor
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-His6-Sumo-CylK-Strep

    Plasmid

    #185962

    Purpose

    Expresses His6-Sumo-CylK-Strep in E. coli for refolding protein preparation

    Depositor

    Emily Balskus

    Article

    Braffman et al Elife. 2022 Feb 25;11. pii: 75761. doi:

    Insert

    CylK
    

    Use

    Tags

    His6-Sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPBO.ACT002

    Plasmid

    #182712

    Purpose

    aTc inducible dCasRx-IF1

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    dCasRx
    

    Use

    CRISPR

    Tags

    3x(GGGS)-Escherichia Coli Initiation Factor 1

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pTet

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. FAK C-term

    Plasmid

    #186147

    Purpose

    For expresseion of recombinant FAK c-terminal domain in E. coli

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSF-1b-S19-T48C

    Plasmid

    #128597

    Purpose

    Expresses tagless S19-T48C mutant 30S ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S19 (rpsS E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T48C

    Promoter

    T7

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSF-1b-S3-M129C

    Plasmid

    #132949

    Purpose

    Expresses tagless S3-M129C mutant ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    E.coli ribosomal protein S3 (rpsC E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    M129C

    Promoter

    T7

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSF-1b-S15-T79C

    Plasmid

    #133048

    Purpose

    Expresses tagless S15-T79C mutant ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    S15 E.coli ribosomal protein (rpsO E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T79C

    Promoter

    T7

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CcCDP_p15A

    Plasmid

    #179272

    Purpose

    Expresses CcCDP in E. coli BL21(DE3)

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

    Insert

    cellodextrin phosphorylase
    

    Use

    Synthetic Biology

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lacO

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET19b-DAD2 cPGFP(A149)

    Plasmid

    #165998

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    DAD2 cPGFP(A149)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET19b-rShHTL7 cPGFP(E167)

    Plasmid

    #166004

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rShHTL7 cPGFP(E167)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET19b-rShHTL7 cPGFP(D165)

    Plasmid

    #166003

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rShHTL7 cPGFP(D165)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET19b-rShHTL7 cPGFP(C164)

    Plasmid

    #166002

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rShHTL7 cPGFP(C164)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET19b-DAD2 cPGFP(N180)

    Plasmid

    #166000

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    DAD2 cPGFP(N180)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET19b-DAD2 cPGFP(A165)

    Plasmid

    #165999

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    DAD2 cPGFP(A165)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET19b-HisDAD2 cPGFP(G164)

    Plasmid

    #165997

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    HisDAD2 cPGFP(G164)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET19b-rShHTL7 cPGFP(L166)

    Plasmid

    #165995

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rShHTL7 cPGFP(L166)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET19b-DAD2 cPGFP(G164)

    Plasmid

    #165993

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    DAD2 cPGFP(G164)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pACYCDuet1-tglCDEF

    Plasmid

    #134677

    Purpose

    Expresses TglC, TglD, TglE, TglF in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Inserts

    TglC
    TglD
    TglE
    TglF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pACYCDuet1-tglB

    Plasmid

    #134676

    Purpose

    Expresses His6-TglBin E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Insert

    TglB
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRSFDuet1-tglA

    Plasmid

    #134673

    Purpose

    Expresses His6-TglA in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Insert

    TglA
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pACYCDuet1-ammA(∆Trp56)

    Plasmid

    #134683

    Purpose

    Expresses His6-AmmA(∆Trp56) in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Insert

    ammA(delW56)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    deletion of W56

    Promoter

    T7

    Available since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28b-tglF

    Plasmid

    #134681

    Purpose

    Expresses His6-TglF in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Insert

    TglF
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28b-GFP-tglG

    Plasmid

    #134679

    Purpose

    Expresses His6-GFP-TglG fusion in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Insert

    TglG
    

    Use

    Tags

    His6-GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET15b-tglHI

    Plasmid

    #134672

    Purpose

    Expresses His6-TglH and TglI in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Inserts

    TglH
    TglI
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAL- S. pombe TSC1 (1-431)

    Plasmid

    #132616

    Purpose

    expresses S. pombe TSC1 (1-431) in E. coli

    Depositor

    Wenqing Xu

    Article

    Sun et al Nat Commun. 2013;4:2135. doi: 10.1038/nc

    Insert

    TSC1 (1-431)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCDB325

    Plasmid

    #113669

    Purpose

    expresses Ulp1_R1 in Escherichia coli

    Depositor

    Christopher Bahl

    Article

    Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

    Insert

    Ulp1_R1
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    contains solubility enhancing mutations

    Promoter

    T7

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMAL-SsoPCNA3

    Plasmid

    #107069

    Purpose

    Expresses S. solfataricus PCNA3 fused to MBP in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    S. solfataricus PCNA3
    

    Use

    Tags

    6xHis-thrombin cleavage site and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCML 221

    Plasmid

    #98587

    Purpose

    pMS2-CYFP. Expresses MS2 protein-CYFP fusion in E. coli for TriFC assay (kanR).

    Depositor

    Lydia Contreras

    Article

    Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

    Insert

    MS2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pLacO

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. CTL2

    Plasmid

    #92212

    Purpose

    Expresses C-Terminal segment of C. elegans Piezo1 in E. coli

    Depositor

    Douglas Rees

    Article

    Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

    Insert

    CTL2 of PIEZO1
    

    Use

    Tags

    6x Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAM5084

    Plasmid

    #85105

    Purpose

    Ptrc-ECFP-KaiC fusion cloned into Km resistance gene of pACYC177. To look for KaiC localization in E. coli.

    Depositor

    Susan Golden

    Article

    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

    Insert

    ecfp-KaiC
    

    Use

    Tags

    ECFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pPD1cR

    Plasmid

    #86970

    Purpose

    Expresses PCNA1 variant (G108C) fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA1 variant (G108C) fused to phosphite dehydrogenase and putidaredoxin reductase
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH, G108C in PCNA1

    Promoter

    T7 promoter

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge