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Showing: 7951 - 8000 of 23359 results

  1. pZNF423.1.0-gDNA

    Plasmid
    #113790
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF423
    Depositor
    Kevin White
    Insert
    ZNF423 (ZNF423 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113790-plasmid-map-sequence-id-230593

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  3. pMLX.1.0-gDNA

    Plasmid
    #112466
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MLX
    Depositor
    Kevin White
    Insert
    MLX (MLX Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112466-plasmid-map-sequence-id-230496

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  9. pFETCh_HHEX

    Plasmid
    #72353
    Purpose
    Donor vector for 3' FLAG tag of human HHEX
    Depositor
    Richard M. Myers, Eric Mendenhall
    Insert
    HHEX Homology Arms (HHEX Human)
    Use
    CRISPR
    Tags
    3XFLAG-P2A-NeoR
    Expression
    Mutation
    Promoter
    Available since
    June 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    72353-plasmid-map-sequence-id-149456

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  10. pTFCP2.1.0-gDNA

    Plasmid
    #112404
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFCP2
    Depositor
    Kevin White
    Insert
    TFCP2 (TFCP2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112404-plasmid-map-sequence-id-230259

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  13. pREST.1.0-gDNA

    Plasmid
    #113774
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor REST
    Depositor
    Kevin White
    Insert
    REST (REST Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113774-plasmid-map-sequence-id-230561

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  14. psLIB U6

    Plasmid
    #229956
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. Has empty U6-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    229956-plasmid-map-sequence-id-461573

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  17. pDest_U6_A2_CG2_T1snp_gRNA

    Plasmid
    #213789
    Purpose
    pDEL114; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type I (SNP) sgRNA
    Depositor
    William Talbot
    Insert
    nrg1 type I SNP sgRNA (nrg1 Zebrafish)
    Use
    Tol2 destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    213789-plasmid-map-sequence-id-421038

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  19. LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS

    Plasmid
    #132927
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Insert
    humanized SpCas9
    Use
    CRISPR and Synthetic Biology; Pmagic gateway entr…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    132927-plasmid-map-sequence-id-314451

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  20. pMSX2.1.0-gDNA

    Plasmid
    #113793
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MSX2
    Depositor
    Kevin White
    Insert
    MSX2 (MSX2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    113793-plasmid-map-sequence-id-241255

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  21. pTOX2.1.0-gDNA

    Plasmid
    #113787
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TOX2
    Depositor
    Kevin White
    Insert
    TOX2 (TOX2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113787-plasmid-map-sequence-id-230586

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  22. pTSHZ2.1.0-gDNA

    Plasmid
    #113778
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TSHZ2
    Depositor
    Kevin White
    Insert
    TSHZ2 (TSHZ2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113778-plasmid-map-sequence-id-230569

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  23. pZNF462.1.0-gDNA

    Plasmid
    #112476
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF462
    Depositor
    Kevin White
    Insert
    ZNF462 (ZNF462 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112476-plasmid-map-sequence-id-230510

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  24. pZNF197.1.0-gDNA

    Plasmid
    #112453
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF197
    Depositor
    Kevin White
    Insert
    ZNF197 (ZNF197 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112453-plasmid-map-sequence-id-230469

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  25. pMYNN.1.0-gDNA

    Plasmid
    #112450
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MYNN
    Depositor
    Kevin White
    Insert
    MYNN (MYNN Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112450-plasmid-map-sequence-id-230463

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  26. pZNF639.1.0-gDNA

    Plasmid
    #112441
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF639
    Depositor
    Kevin White
    Insert
    ZNF639 (ZNF639 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112441-plasmid-map-sequence-id-230401

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  27. pZNF23.1.0-gDNA

    Plasmid
    #112421
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF23
    Depositor
    Kevin White
    Insert
    ZNF23 (ZNF23 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112421-plasmid-map-sequence-id-230301

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  28. pTBPL1.1.0-gDNA

    Plasmid
    #112412
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TBPL1
    Depositor
    Kevin White
    Insert
    TBPL1 (TBPL1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112412-plasmid-map-sequence-id-230271

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  29. pNR1D2.1.0-gDNA

    Plasmid
    #112410
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR1D2
    Depositor
    Kevin White
    Insert
    NR1D2 (NR1D2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112410-plasmid-map-sequence-id-230269

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  30. pZNF354B.1.0-gDNA

    Plasmid
    #112405
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF354B
    Depositor
    Kevin White
    Insert
    ZNF354B (ZNF354B Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112405-plasmid-map-sequence-id-230264

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  31. PB-EEA-5g-OSK2M2L1-PGK-Puro

    Plasmid
    #102909
    Purpose
    PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102909-plasmid-map-sequence-id-200136

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  32. PB-GG-OMKSL-PGK-Puro

    Plasmid
    #102894
    Purpose
    PiggyBac transposon system construct with 10 concatenated U6 promoter driven transcriptional cassettes targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Contains PGK-puro selection cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    OMKSL-gRNAs-PGK-Puro (POU5F1, MYC, KLF4, SOX2, LIN28A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102894-plasmid-map-sequence-id-198285

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  33. GG-EBNA-OMKSL-PP

    Plasmid
    #102899
    Purpose
    EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    OMKSL-gRNAS-PGK-puro (POU5F1, MYC, KLF4, SOX2, LIN28A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102899-plasmid-map-sequence-id-198366

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  42. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    118154-plasmid-map-sequence-id-239465

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  45. CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

    Plasmid
    #225755
    Purpose
    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection
    Depositor
    Paul Blainey
    Article
    Walton et al bioRxiv 2024.03.17.585235
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    225755-plasmid-map-sequence-id-446065

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  50. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118158
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118158-plasmid-map-sequence-id-240404

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Showing: 7951 - 8000 of 23359 results