We narrowed to 8,811 results for: CAG

1. AAV-GfaABC1D-ERCreER-4x6T

   Plasmid

   #196411

   Purpose

   Astrocytic expression of ERT2-Cre-ERT2 recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Insert

   ERT2-Cre-ERT2
   

   Use

   AAV; Astrocyte-selective

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and GfaABC1D

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-BsaIx2-DD-Cas9

   Plasmid

   #75380

   Purpose

   Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1

   Depositor

   Michele Calos

   Article

   Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

   Insert

   DD-SpCas9
   

   Use

   CRISPR

   Tags

   FKBP12 L106P DD

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT076

   Plasmid

   #137880

   Purpose

   Donor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFP

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-CA-rtTA Adv

   Plasmid

   #20910

   Purpose

   piggyBac vector for expression of reverse tetracycline transactivator from a CAG constitutive promoter

   Depositor

   Andras Nagy

   Article

   Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

   Insert

   rtTA-ON-Adv
   

   Use

   piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB-Neo-Mito-DsRed

   Plasmid

   #210328

   Purpose

   Overexpression of Mito-DsRed

   Depositor

   Li Yu

   Article

   Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

   Insert

   Mito-DsRed
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPBbsr-JNK KTR-mCherry

   Plasmid

   #115493

   Purpose

   Expression vector for the JNK activity reporter JNK KTR-mCherry in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

   Insert

   JNK KTR-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   mCherry G229L, mCherry Y298 deleted

   Promoter

   CAG

   Available since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-FLEX-FLIM-AKAR

   Plasmid

   #60445

   Purpose

   Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent manner in an AAV backbone

   Depositor

   Bernardo Sabatini

   Article

   Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

   Insert

   FLIM-AKAR
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCX-SpiCee-NLS

   Plasmid

   #140900

   Purpose

   mammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   SpiCee-NLS
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-FLEX-EGFP-mir30(Scn9a)

   Plasmid

   #79672

   Purpose

   Cre-dependent knockdown of Scn9a (Nav1.7)

   Depositor

   Scott Sternson

   Article

   Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

   Insert

   EGFP (Scn9a jellyfish, Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCX-SpiCee-NES

    Plasmid

    #140901

    Purpose

    mammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    SpiCee-NES
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCX-SponGee

    Plasmid

    #134775

    Purpose

    mammalian expression of SponGee, a buffer of cGMP that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

    Insert

    SponGee
    

    Use

    Tags

    FLAG and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIG2-Flag-mNuak1 WT

    Plasmid

    #168507

    Purpose

    For expression of Nuak1 (AMPK-related kinase also called ARK5/Omphk1)

    Depositor

    Franck Polleux

    Article

    Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

    Insert

    Nuak1 (Nuak1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPBbsr2-Lifeact-miRFP703

    Plasmid

    #178530

    Purpose

    Expresses Lifeact-miRFP703 in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Lifeact-miRFP703
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. SQT1665

    Plasmid

    #78744

    Purpose

    Human expression plasmid for LbCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCX-NTOM-SpiCee

    Plasmid

    #140902

    Purpose

    mammalian expression of NTOM-SpiCee, a mitochondria-targeted variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    NTOM-SpiCee
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. SQT1659

    Plasmid

    #78743

    Purpose

    Human expression plasmid for AsCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmROSA26-attP50-Neo-mKate2-3xNLS-attP50

    Plasmid

    #183609

    Purpose

    Targeting vector for mouse ROSA26 locus to create landing pad cell line. Introduces attP50 sites flanking a Neo gene driven by endogenous locus (confers G418 resistance) and a CAG-mKate2-3xNLS.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Inserts

    Neo
    mKate2-3xNLS
    

    Use

    Mouse Targeting

    Tags

    3xNLS

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCX-Lyn-SpiCee

    Plasmid

    #140837

    Purpose

    mammalian expression of Lyn-SpiCee, a lipid raft-targeted variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    Lyn-SpiCee
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT2ADW-lox-tdKeima-NES-lox-6011nls(ERK)-P2Av3-RGECO1

    Plasmid

    #162486

    Purpose

    For generating transgenic mice expressing ERK biosensor and RGECO1

    Depositor

    Michiyuki Matsuda

    Article

    Imanishi et al Am J Pathol. 2020 Oct 15. pii: S0002-944

    Insert

    ERK biosensor based on FRET, and RGECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEM705 Gs

    Plasmid

    #109350

    Purpose

    Wildtype Gαs

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    GαS (GNAS Human)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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