We narrowed to 4,074 results for: p2a

1. pOT_7-lenti-EFS-SS1-BBz-2A-puro

   Plasmid

   #217990

   Purpose

   Expresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Xue et al bioRxiv 2024.11.27.625752

   Insert

   SS1-BBz CAR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFETCh_AHR

   Plasmid

   #101071

   Purpose

   Donor vector for 3' FLAG tag of human AHR

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   AHR homology arms (AHR Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC034 - LwCas13a-msfGFP-2A-Blast

   Plasmid

   #91924

   Purpose

   Expresses active LwCas13a for mammalian RNA targeting with Blasticidin selection marker

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS and msfGFP-NLS-3xHA, P2A, Blasticidin

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRE-SEAP

   Plasmid

   #210511

   Purpose

   SEAP reporter vector for LAUNCHER system

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   secreted alkaline phosphatase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNS35 (multiAsCas12a piggyBac)

   Plasmid

   #217336

   Purpose

   piggyBac expression of multiAsCas12a

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   multiAsCas12a
   

   Use

   CRISPR

   Tags

   6xMycNLS and HA-SV40NLS-P2A-TagBFP2

   Expression

   Mammalian

   Mutation

   R1226A/E174R/S542R/K548R

   Promoter

   CAG

   Available since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCH4 (denAsCas12a-KRAB lenti)

   Plasmid

   #217331

   Purpose

   Lentiviral expression of denAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   denAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Expression

   Mutation

   D908A/E174R/S542R/K548R

   Promoter

   UCOE-SFFV

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-NRCH-PE2-BSD

   Plasmid

   #191101

   Purpose

   Lentiviral expression plasmid of NRCH-PE prime editor with P2A-BSD marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   NRCH-PE2-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRosetta_v2

   Plasmid

   #136477

   Purpose

   Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   HygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   PGK

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPBT/EFS-Cas9-PuroTk

   Plasmid

   #170542

   Purpose

   Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Insert

   PuroTK
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-HNF4A-FOXA2-FOXA3

    Plasmid

    #149724

    Purpose

    expressing three liver promoting transcription factors (HNF4A, FOXA2, FOXA3)

    Depositor

    Baisong Lu

    Article

    Lu et al Biomaterials. 2021 Feb;269:120668. doi:

    Insert

    HNF4A-FOXA2-FOXA3
    

    Use

    Lentiviral

    Tags

    P2A, T2A

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cas9-2A-Cre

    Plasmid

    #68468

    Purpose

    co-expressing Cas9 and Cre recombinase

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    Cas9 and Cre recombinase
    

    Use

    CRISPR

    Tags

    P2A-Cre-Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. LRG2.1_Neo

    Plasmid

    #125593

    Purpose

    Lentiviral expression of sgRNA with GFP and neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for sgRNA expression and EFS promoter…

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLC-Flag-CSNK1A1 WT-Puro

    Plasmid

    #123319

    Purpose

    Lentiviral vector for expression of Flag tagged CK1alpha-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

    Insert

    CSNK1A1 (CSNK1A1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Equalizer-L episome

    Plasmid

    #169738

    Purpose

    Equalizer-L on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. T/brachyury(2A-Dre)

    Plasmid

    #140761

    Purpose

    Targeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.

    Depositor

    Hideyuki Okano

    Article

    Serizawa et al Development. 2019 Nov 4;146(21). pii: de

    Insert

    Dre
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Endogenous T/brachyury

    Available since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpCas9-NRTH

    Plasmid

    #198541

    Purpose

    Expresses human codon-optimized SpCas9-NRTH and blasticidin resistance: EFS promoter-SpCas9-NRTH-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRTH
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. GWF243

    Plasmid

    #133608

    Purpose

    NS3a-CAAX-(IRES)-EGFP-DNCR2-TIAM-P2a-BFP-GNCR1-LARG

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    NS3a-CAAX, EGFP-DNCR2-TIAM, BFP-GNCR1-LARG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTS2303-Tier1-CoTC-PPGK-NLuc_TB

    Plasmid

    #169548

    Purpose

    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    CoTC-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1_Cas9-hGem_gRNA2

    Plasmid

    #217659

    Purpose

    expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    sgRNA2 targeting the human AAVS1 safe harbour locus
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFETCh_KDM1A

    Plasmid

    #86260

    Purpose

    Donor vector for 3' FLAG tag of human KDM1A

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    KDM1A homology arms (KDM1A Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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