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pAc5.1-PH-mCherry-Oskar 241-387 (DR) (HK74)

Plasmid

#206462

Purpose

Oskar 240-387 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Oskar 240-387 (osk Fly)

Use

Tags

PH mcherry

Expression

Insect

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Bam isoform A (HK62)

Plasmid

#206466

Purpose

Bam expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Bam (bam Fly)

Use

Tags

PH mcherry

Expression

Insect

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-CCR4 isoform A-mCherry-PH (EB7)

Plasmid

#206473

Purpose

CCR4 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

CCR4 (twin Fly)

Use

Tags

mcherry PH

Expression

Insect

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-OST4-mCherry-CAF1 isoform A (HK184)

Plasmid

#206476

Purpose

CAF1 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

CAF1 (Pop2 Fly)

Use

Tags

OST4 mcherry

Expression

Insect

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_SAT1 (human)

Plasmid

#220477

Purpose

heterologous expression of SSAT1 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

SSAT1 (SAT1 Human)

Use

Tags

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pRER-PhoCl-Insert

Plasmid

#219675

Purpose

Caging at lumenal N-ter of POI: TMEM-PhoCl-MCS in pTREtight2

Depositor

Helge Ewers

Article

Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

Insert

RER-PhoCl

Use

Tags

PhoCl

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 14, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pMCSG7-10xHis-ecRNHI*

Plasmid

#219523

Purpose

bacterial expression of cysteine-free variant of the E. coli ribonuclease HI gene

Depositor

Arthur Palmer

Article

Bhattacharya et al J Biomol NMR. 2024 Jun 10. doi: 10.1007/

Insert

cysteine-free variant of the E. coli ribonuclease HI gene

Use

Tags

N terminal 10xHis Tag

Expression

Bacterial

Mutation

Cysteine free variant

Promoter

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-ARMS1-ProLink2-MRGPRX3

Plasmid

#213962

Purpose

Expresses MRGPRX3 in mammalian cells

Depositor

Christa Müller

Article

Al Hamwi et al Commun Biol. 2024 Jan 6;7(1):52. doi: 10

Insert

MRGPRX3 (MRGPRX3 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pME rras_Q61R

Plasmid

#201057

Purpose

Gateway middle entry vector expressing constitutively actve zebrafish rras

Depositor

Sarah Childs

Article

Greysson-Wong et al Development. 2023 Sep 15;150(18):dev2018

Insert

RRas_Q61R (rras Zebrafish)

Use

Zebrafish expression

Tags

Expression

Mutation

Mutation Q61R, constitutively active

Promoter

none

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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2M-Mx-FLAG

Plasmid

#206942

Purpose

Mammalian Expression of 2M-Mx-FLAG EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, MxEnc

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-hArfaptin1

Plasmid

#206402

Purpose

Mammalian expression of hArfaptin1

Depositor

Hye-Won Shin, Kazuhisa Nakayama

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

Arfaptin1 (ARFIP1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-hArfaptin2

Plasmid

#206403

Purpose

Mammalian expression of hArfaptin2

Depositor

Hye-Won Shin, Kazuhisa Nakayama

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

Arfaptin2 (ARFIP2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2A D197,199N

Plasmid

#195704

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain with D197, 199N mutations

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 124-211 of mouse SYT9 with D1…

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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1M-Mx-FLAG

Plasmid

#206941

Purpose

Mammalian Expression of 1M-Mx-FLAG EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, MxEnc

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
1M-Qt-FLAG

Plasmid

#206938

Purpose

Mammalian Expression of 1M-Qt-FLAG EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, QtEnc

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
1M-Tm-BC2

Plasmid

#206943

Purpose

Mamamlian Expression of 1M-Tm-BC2Tag EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, TmEnc

Use

Tags

BC2

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
3M-Qt-FLAG

Plasmid

#206940

Purpose

Mammalian Expression of 3M-Qt-FLAG EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, SeSmtA, TaEC1, QtEnc

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
2M-Qt-FLAG

Plasmid

#206939

Purpose

Mammalian Expression of 2M-Qt-FLAG EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, QtEnc

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-C-TEV-HAT

Plasmid

#202597

Purpose

Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Use

Tags

TEV cleavable site (ENLYFQG) and poly-histidine a…

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-N-TEV-HAT

Plasmid

#202598

Purpose

Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Use

Tags

TEV cleavable site (ENLYFQG) and poly-histidine a…

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge