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pT7-EGFP-HsGIGYF2_1-180-LFItoDDD_AE

Plasmid

#148582

Purpose

Mammalian Expression of HsGIGYF2_1-180-LFItoDDD

Depositor

Elisa Izaurralde

Publication

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_1-180-LFItoDDD (GIGYF2 Human)

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsGIGYF2_35-105-E46AE47FN91A-GB1_AF

Plasmid

#148686

Purpose

Bacterial Expression of HsGIGYF2_35-105-E46AE47FN91A

Depositor

Elisa Izaurralde

Publication

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_35-105-E46AE47FN91A (GIGYF2 Human)

Expression

Bacterial

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF1_1-177-YYMLtoAAAA_AE

Plasmid

#148575

Purpose

Mammalian Expression of HsGIGYF1_1-177-YYMLtoAAAA

Depositor

Elisa Izaurralde

Publication

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_1-177-YYMLtoAAAA (GIGYF1 Human)

Expression

Mammalian

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMS22

Plasmid

#129710

Purpose

Expresses Escherichia coli RssB E134G:E135S:E136G:E137S variant as an N-terminal 10His fusion

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

Escherichia coli RssB-GSGS variant (rssB Escherichia coli MG1655)

Tags

10-Histidine tag cleavable with PreScission Prote…

Expression

Bacterial

Mutation

RssB E134G:E135S:E136G:E137S

Promoter

T7

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMT Draper-Ala11-GFP

Plasmid

#126654

Purpose

Inducible expression of Draper-Ala11-GFP mutant in S2 cells

Depositor

Ron Vale

Publication

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper cytoplasmi Tyr residues mutated to Ala

Expression

Insect

Mutation

Cytoplasmic Tyr residues to Ala

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMT Shark-TagBFP

Plasmid

#126658

Purpose

Inducible expression of Shark-TagBFP in S2 cells

Depositor

Ron Vale

Publication

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Shark

Expression

Insect

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL2154

Plasmid

#71204

Purpose

pT181 STAR Antisense Expression Plasmid

Depositor

Julius Lucks

Publication

Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

Insert

pT181 STAR Antisense

Use

Synthetic Biology

Promoter

BBa_J23119

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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V5-W279A-short-UBASH3A

Plasmid

#192086

Purpose

Mammalian expression of human W279A short-form UBASH3A with V5 tag

Depositor

Patrick Concannon

Publication

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Tags

V5

Expression

Mammalian

Mutation

W279A

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pME VIVIT NS

Plasmid

#188699

Purpose

Gateway middle element encoding the VIVIT peptide (no stop codon)

Depositor

David Tobin

Publication

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

VIVIT

Use

Synthetic Biology

Expression

Mammalian

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLV VEET-GFP

Plasmid

#188706

Purpose

Lentivirus transfer plasmid encoding VEET-GFP

Depositor

David Tobin

Publication

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

VEET-GFP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL002

Plasmid

#71202

Purpose

Antisense Control Plasmid

Depositor

Julius Lucks

Publication

Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23119

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pSA10(gfcD-10xHis)

Plasmid

#176192

Purpose

gfcD protein expression

Depositor

Mark Saper

Publication

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Tags

10xHis

Expression

Bacterial

Promoter

Ptac

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMCSG7(gfcB)

Plasmid

#176196

Purpose

gfcB protein expression

Depositor

Mark Saper

Publication

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcB

Tags

6xHis-TeV

Expression

Bacterial

Promoter

T7

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMCSG26(gfcD)

Plasmid

#176198

Purpose

gfcD protein expression

Depositor

Mark Saper

Publication

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pGAL80

Plasmid

#165825

Purpose

GAL4 inhibitor coding sequence (CDS)

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

GAL80

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pQS

Plasmid

#165826

Purpose

QF inhibitor coding sequence (CDS)

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

QS

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBSR

Plasmid

#165838

Purpose

blasticidin S resistance marker

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

bsr

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pTP10

Plasmid

#165797

Purpose

Drosophila TP10 poly(a) terminator element

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

TP10

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAdh 3'UTR Short

Plasmid

#165800

Purpose

Drosophila HSP70B 3'UTR sequence

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Hsp70 3'UTR

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDmVasa

Plasmid

#165783

Purpose

Drosohphila vasa germline promoter and 5'UTR

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vasa promoter

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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