We narrowed to 7,280 results for: 11

1. pCDNA3_cAMPFIRE-L

   Plasmid

   #182279

   Purpose

   Mammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.

   Depositor

   Haining Zhong

   Article

   Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

   Insert

   cAMPFIRE-L (cAMP sensor)
   

   Expression

   Mammalian

   Available Since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-Kif5b-Myc

   Plasmid

   #186614

   Purpose

   Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform B fused to Myc tag

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Kinesin Heavy Chain isoform 5b (KIF5B Human)
   

   Use

   Lentiviral

   Tags

   Myc tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSPIH6

   Plasmid

   #190676

   Purpose

   E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)

   Depositor

   John Vederas

   Article

   Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi

   Inserts

   Small ubiquitin-like modifier
   Mxe GyrA intein with C-terminal chitin binding domain and His tag
   

   Tags

   Chitin binding domain, His tag, and N-terminal Hi…

   Expression

   Bacterial

   Mutation

   Added C-terminal his tag and None

   Promoter

   T7

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21 gamma-tubulin

   Plasmid

   #101825

   Purpose

   production of gamma-tubulin in bacteria

   Depositor

   Ana Carrera, Maria Alvarado-Kristensson

   Article

   Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

   Insert

   gamma-tubulin 1 (TUBG1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Whole protein

   Promoter

   T7

   Available Since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. nLuc-PTHR1

   Plasmid

   #162577

   Purpose

   Expresses N-terminally NanoLuc-Tagged PTHR1 in mammalian cells

   Depositor

   Sam Gellman

   Article

   Yu et al ACS Chem Biol. 2022 Nov 18;17(11):3148-3

   Insert

   Parathyroid Hormone Receptor 1 (PTH1R Human)
   

   Tags

   Nanoluc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3544 pMIG Bcl-2

   Plasmid

   #8793

   Depositor

   Stanley Korsmeyer

   Article

   Cheng et al Mol Cell 2001 Sep;8(3):705-11.

   Insert

   Bcl-2 (BCL2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmStrawberry-Atg4BC74A

   Plasmid

   #21076

   Depositor

   Tamotsu Yoshimori

   Article

   Fujita et al Mol Biol Cell. 2008 Nov . 19(11):4651-9.

   Insert

   Atg4B(C74A) (Atg4b Mouse)
   

   Tags

   mStrawberry

   Expression

   Mammalian

   Mutation

   220-222 TGC(Cys) is changed to GCA (Ala)

   Available Since

   June 8, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. STAT3(Y705F)-TAL-Luc

   Plasmid

   #46933

   Purpose

   Luciferase Reporter for mutant STAT3

   Depositor

   Afshin Dowlati

   Article

   Dabir et al Mol Cancer Res. 2009 Nov;7(11):1854-60.

   Insert

   STAT3(Y705F) (STAT3 Human)
   

   Use

   Luciferase

   Tags

   FLAG

   Mutation

   Tyrosine 705 to Phenylalanine

   Promoter

   TA Luciferase

   Available Since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pCMV-GABRA1-IRES2-EGFP

   Plasmid

   #170820

   Purpose

   human GABAA receptors alpha1 subunit and IRES2-EGFP

   Depositor

   Tingwei Mu

   Article

   Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

   Insert

   Human GABAA receptor alpha1 subunit (GABRA1 Human)
   

   Tags

   IRES2-EGFP

   Expression

   Mammalian

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTP-PCR C3

    Plasmid

    #189620

    Purpose

    Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:

    Insert

    Reduced Mitochondrial Genome of Thalassiosira pseudonana
    

    Use

    Synthetic Biology; Diatom expression

    Expression

    Bacterial and Yeast

    Mutation

    Removed repeat region, 10-kbp deletion, and minor…

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCV Puro SCRIB WT

    Plasmid

    #88886

    Purpose

    SCRIB WT in MSCV backbone

    Depositor

    Senthil Muthuswamy

    Article

    Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

    Insert

    SCRIB (SCRIB Human)
    

    Use

    Retroviral

    Tags

    N/A

    Mutation

    N/A

    Promoter

    MSCV promoter

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEasyG2_hph

    Plasmid

    #184915

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEasyG2_mic

    Plasmid

    #184916

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas9(D10A)

    Plasmid

    #74495

    Purpose

    Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

    Insert

    Cas9 D10A
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    D10A

    Promoter

    pTeto

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. DT480

    Plasmid

    #80413

    Purpose

    Expresses 480 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

    Depositor

    Thomas Cooper

    Article

    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

    Insert

    human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3_Sec61B-V5-APEX2

    Plasmid

    #83411

    Purpose

    Expressed C-term tagged APEX2 on Sec61B in mammalian cell

    Depositor

    Hyun-Woo Rhee

    Article

    Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

    Insert

    Sec61B-V5-APEX2 (SEC61B Human, Synthetic)
    

    Tags

    APEX2 and V5

    Expression

    Mammalian

    Promoter

    CMV-F

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Cyto-ExRai-CKAR2

    Plasmid

    #236097

    Purpose

    Cytosol-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

    Depositor

    Jin Zhang

    Article

    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

    Insert

    ExRai-CKAR2-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cx43-msfGFP

    Plasmid

    #69007

    Purpose

    Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Gja1 (Gja1 Rat)
    

    Tags

    monomerized superfolder GFP

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3 NQO1-Flag

    Plasmid

    #61729

    Purpose

    Expresses human NQO1 Flag tagged at C-terminus

    Depositor

    Yosef Shaul

    Article

    Tsvetkov et al J Biol Chem. 2011 Mar 18;286(11):8839-45

    Insert

    NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28-His6-Ubc9

    Plasmid

    #133909

    Purpose

    Bacterial expression of His6-Ubc9

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    SUMO-conjugating enzyme UBC9 (UBE2I Human)
    

    Tags

    Thrombin-cleavable His6-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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